Molecular interaction of ctDNA and HSA with sulfadiazine sodium by multispectroscopic methods and molecular modeling

Geng, Shaoguang; Liu, Guosheng; Li, Wei; Cui, Fengling

doi:10.1002/bio.2457

Cited by 23 publications

(13 citation statements)

References 30 publications

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“…An appreciable decrease in emission intensities was observed upon the addition of 2-Mn and 2-Fe to the EB-DNA system ( Figure 4 ). It is known that the binding constant of EB with DNA is 5.16 × 10 5 L·mol −1 [ 46 ], so the smaller binding constant between porphyrins and DNA indicates that replacing EB from the complex was impossible. As a result, the second explanation seems more reasonable.…”

Section: Resultsmentioning

confidence: 99%

Water-soluble Manganese and Iron Mesotetrakis(carboxyl)porphyrin: DNA Binding, Oxidative Cleavage, and Cytotoxic Activities

Shi

Jiang

et al. 2017

Molecules

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Two new water-soluble metal carboxyl porphyrins, manganese (III) meso-tetrakis (carboxyl) porphyrin and iron (III) meso-tetrakis (carboxyl) porphyrin, were synthesized and characterized. Their interactions with ct-DNA were investigated by UV-Vis titration, fluorescence spectra, viscosity measurement and CD spectra. The results showed they can strongly bind to ct-DNA via outside binding mode. Electrophoresis experiments revealed that both complexes can cleave pBR322 DNA efficiently in the presence of hydrogen peroxide, albeit 2-Mn exhibited a little higher efficiency. The inhibitor tests suggest the oxidative DNA cleavage by these two complexes may involve hydroxyl radical active intermediates. Notably, 2-Mn exhibited considerable photocytotoxicity against Hep G2 cell via triggering a significant generation of ROS and causing disruption of MMP after irradiation.

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Section: Resultsmentioning

confidence: 99%

Water-soluble Manganese and Iron Mesotetrakis(carboxyl)porphyrin: DNA Binding, Oxidative Cleavage, and Cytotoxic Activities

Shi

Jiang

et al. 2017

Molecules

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“…In order to gain a better understanding on the conformational behavior of BPTI/BFDI/BDTI on binding to HSA, 3D fluorescence spectroscopy was also applied . The 3D fluorescence spectra of HSA were investigated by comparing spectral changes in the absence and presence of BPTI/BFDI/BDTI and are shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…UV-vis spectroscopy served as complementary evidence, along with synchronous spectroscopy, to clearly support conformational changes in protein. In order to gain a better understanding on the conformational behavior of BPTI/BFDI/BDTI on binding to HSA, 3D fluorescence spectroscopy was also applied (31,32). The 3D fluorescence spectra of HSA were investigated by comparing spectral changes in the absence and presence of BPTI/BFDI/BDTI and are shown in Fig.…”

Section: Conformational Investigationsmentioning

confidence: 99%

Interaction of human serum albumin with novel imidazole derivatives studied by spectroscopy and molecular docking

et al. 2015

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This study was a detailed characterization of the interaction of a series of imidazole derivatives with a model transport protein, human serum albumin (HSA). Fluorescence and time-resolved fluorescence results showed the existence of a static quenching mode for the HSA-imidazole derivative interaction. The binding constant at 296 K was in the order of 10(4) M(-1), showing high affinity between the imidazole derivatives and HSA. A site marker competition study combined with molecular docking revealed that the imidazole derivatives bound to subdomain IIA of HSA (Sudlow's site I). Furthermore, the results of synchronous, 3D, Fourier transform infrared, circular dichroism and UV-vis spectroscopy demonstrated that the secondary structure of HSA was altered in the presence of the imidazole derivatives. The specific binding distance, r, between the donor and acceptor was obtained according to fluorescence resonance energy transfer.

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“…The binding constant K b increased with the increasing temperature, indicating that rising temperature contributed to the binding of ligand L and La (III) complex with DNA and the La (III) complex has much stronger binding ability than ligand L . Compared to the binding constant of EB with DNA (5.16 × 10 5 L mol −1 ) [ 35 ], the ligand L and La (III) complex could compete against EB and replace the intercalated EB from the DNA-EB complex system.…”

Section: Resultsmentioning

confidence: 99%

Synthesis, Characterization, DNA Interaction, and Antitumor Activities of La (III) Complex with Schiff Base Ligand Derived from Kaempferol and Diethylenetriamine

Wang

Huang

Zhang

et al. 2014

Bioinorganic Chemistry and Applications

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A novel La (III) complex, [LaL(H2O)3]NO3 ·3H2O, with Schiff base ligand L derived from kaempferol and diethylenetriamine, has been synthesized and characterized by elemental analysis, IR, UV-visible, 1H NMR, thermogravimetric analysis, and molar conductance measurements. The fluorescence spectra, circular dichroism spectra, and viscosity measurements and gel electrophoresis experiments indicated that the ligand L and La (III) complex could bind to CT-DNA presumably via intercalative mode and the La (III) complex showed a stronger ability to bind and cleave DNA than the ligand L alone. The binding constants (K b) were evaluated from fluorescence data and the values ranged from 0.454 to 0.659 × 105 L mol−1 and 1.71 to 17.3 × 105 L mol−1 for the ligand L and La (III) complex, respectively, in the temperature range of 298–310 K. It was also found that the fluorescence quenching mechanism of EB-DNA by ligand L and La (III) complex was a static quenching process. In comparison to free ligand L, La (III) complex exhibited enhanced cytotoxic activities against tested tumor cell lines HL-60 and HepG-2, which may correlate with the enhanced DNA binding and cleaving abilities of the La (III) complex.

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Molecular interaction of ctDNA and HSA with sulfadiazine sodium by multispectroscopic methods and molecular modeling

Cited by 23 publications

References 30 publications

Water-soluble Manganese and Iron Mesotetrakis(carboxyl)porphyrin: DNA Binding, Oxidative Cleavage, and Cytotoxic Activities

Water-soluble Manganese and Iron Mesotetrakis(carboxyl)porphyrin: DNA Binding, Oxidative Cleavage, and Cytotoxic Activities

Interaction of human serum albumin with novel imidazole derivatives studied by spectroscopy and molecular docking

Synthesis, Characterization, DNA Interaction, and Antitumor Activities of La (III) Complex with Schiff Base Ligand Derived from Kaempferol and Diethylenetriamine

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