Interaction of COP1 and UVR8 regulates UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis

Favory, Jean-Jacques; Stec, Agnieszka; Gruber, Henriette; Rizzini, Luca; Oravecz, Attila; Funk, Markus; Albert, Andreas; Cloix, Catherine; Jenkins, Gareth I.; Oakeley, Edward J.; Seidlitz, Harald K.; Nagy, Ferenc; Ulm, Roman

doi:10.1038/emboj.2009.4

Cited by 569 publications

(1,056 citation statements)

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“…UVR8 and COP1 interact directly and rapidly in the nucleus in planta after UV-B light exposure. It is proposed that this very early step in UV-B light signaling coordinates responses, ensuring UV-B acclimation and protection (Favory et al, 2009). In maize plants, UV-B light exposure induced RPL10 expression, in agreement with our previous microarray results (Fig.…”

Section: Discussionsupporting

confidence: 82%

Plant L10 Ribosomal Proteins Have Different Roles during Development and Translation under Ultraviolet-B Stress

et al. 2010

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(J.B., A.L.B.) Ribosomal protein L10 (RPL10) proteins are ubiquitous in the plant kingdom. Arabidopsis (Arabidopsis thaliana) has three RPL10 genes encoding RPL10A to RPL10C proteins, while two genes are present in the maize (Zea mays) genome (rpl10-1 and rpl10-2). Maize and Arabidopsis RPL10s are tissue-specific and developmentally regulated, showing high levels of expression in tissues with active cell division. Coimmunoprecipitation experiments indicate that RPL10s in Arabidopsis associate with translation proteins, demonstrating that it is a component of the 80S ribosome. Previously, ultraviolet-B (UV-B) exposure was shown to increase the expression of a number of maize ribosomal protein genes, including rpl10. In this work, we demonstrate that maize rpl10 genes are induced by UV-B while Arabidopsis RPL10s are differentially regulated by this radiation: RPL10A is not UV-B regulated, RPL10B is down-regulated, while RPL10C is up-regulated by UV-B in all organs studied. Characterization of Arabidopsis T-DNA insertional mutants indicates that RPL10 genes are not functionally equivalent. rpl10A and rpl10B mutant plants show different phenotypes: knockout rpl10A mutants are lethal, rpl10A heterozygous plants are deficient in translation under UV-B conditions, and knockdown homozygous rpl10B mutants show abnormal growth. Based on the results described here, RPL10 genes are not redundant and participate in development and translation under UV-B stress.

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Section: Discussionsupporting

confidence: 82%

Plant L10 Ribosomal Proteins Have Different Roles during Development and Translation under Ultraviolet-B Stress

et al. 2010

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“…Here, we provided solid evidence showing that the degradation of BBX24 in the dark is mediated by COP1 through the 26S proteasome pathway. However, COP1 remains in the nucleus under UV-B radiation [8,12], and our results show that BBX24 accumulates at the same time, suggesting that COP1 does not function as an E3 ubiquitin ligase that targets BBX24 in UV-B responses. This notion was further supported by the evidence that MG132 treatment has little effect on the UV-B-induced BBX24 accumulation ( Figure 2B).…”

Section: Bbx24 Is a Key Uv-b Signaling Component That Interacts Withmentioning

confidence: 77%

“…Briefly, the UV-B signal is perceived by the UV-B receptor UVR8, resulting in rapid accumulation of COP1 and its interaction with UVR8. UVR8-COP1 interaction presumably stabilizes and activates HY5 for its transcriptional activity, leading to UV-B-regulated gene expression and photomorphogenesis [8]. This UV-B signaling cascade is feedback regulated by a set of negative regulators, which may include STO/BBX24, RCD1, and RUP1/RUP2.…”

Section: Bbx24 May Function As a New Component Of The Negative Feedbamentioning

confidence: 99%

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Arabidopsis STO/BBX24 negatively regulates UV-B signaling by interacting with COP1 and repressing HY5 transcriptional activity

et al. 2012

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UV-B (280-315 nm)is an integral part of solar radiation and can act either as a stress inducer or as a developmental signal. In recent years, increasing attention has been paid to the low-fluence UV-B-induced photomorphogenic response and several key players in this response have been identified, which include UVR8 (a UV-B-specific photoreceptor), COP1 (a WD40-repeat-containing RING finger protein), HY5 (a basic zipper transcription factor), and RUP1/2 (two UVR8-interacting proteins). Here we report that Arabidopsis SALT TOLERANCE (STO/BBX24), a known regulator for light signaling in plants, defines a new signaling component in UV-B-mediated photomorphogenesis. The bbx24 mutant is hypersensitive to UV-B radiation and becomes extremely dwarfed under UV-B treatment. By contrast, BBX24 overexpression transgenic lines respond much more weakly to UV-B than the bbx24 and wild-type plants. BBX24 expression is UV-B-inducible and its accumulation under UV-B requires COP1. Co-immunoprecipitation experiments indicate that BBX24 interacts with COP1

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“…UVR8 perceives radiation, triggering the dissociation from its non-active homodimer configuration [137,138]. Following monomerization, UVR8 accumulates in the nucleus and interacts with the positive regulator Constitutively Photomorphogenic 1 (COP1) [139][140][141][142], a WD40/RING-E3 ubiquitin ligase that in non-inductive conditions targets HY5 (Elongated Hypocotyl 5) for proteosome-dependent degradation [143]. HY5 is a key effector of UV-B protection and light photomorphogenic responses [144,145], and it is transcriptionally activated by UV-B in a UVR8-and COP1-dependent manner [146][147][148].…”

Section: Uvr8-mediated Photomorphogenic Mechanisms In Response To Uv-mentioning

confidence: 99%

Grapevine Biotechnology: Molecular Approaches Underlying Abiotic and Biotic Stress Responses

Armijo¹,

Espinoza²,

Loyola³

et al. 2016

Grape and Wine Biotechnology

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Grapevine is one of the most abundant crops worldwide, with varieties destined for fresh and dry consumption, as well as wine production. Unfortunately, grapevine plants are affected by both biotic and abiotic stresses, generating significant economic losses. These conditions can negatively impact grape cultivation at different stages: plant and berry development during pre-and post-harvest, production, fresh fruit processing and export, along with wine quality. Most of the grapevine varieties are susceptible to several pathogens and within this chapter, particular attention is given to fungi (Botrytis cinerea and Erysiphe necator) and viruses, since they are a worldwide concern. Within the latter, special focus is given to the grapevine leafroll disease, a complex and destructive infection. On the other hand, abiotic stress is also relevant in grapevine, and in this chapter it will be exemplified by UV-B radiation and its impact on growth and fruit development, plant adaptive responses and its relationship with the quality of grape berries for winemaking. The main biotic and abiotic grapevine stress factors are reviewed in this chapter, considering a special focus on biotechnological approaches carried out in order to address them and minimize their detrimental consequences.

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Interaction of COP1 and UVR8 regulates UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis

Cited by 569 publications

References 44 publications

Plant L10 Ribosomal Proteins Have Different Roles during Development and Translation under Ultraviolet-B Stress

Plant L10 Ribosomal Proteins Have Different Roles during Development and Translation under Ultraviolet-B Stress

Arabidopsis STO/BBX24 negatively regulates UV-B signaling by interacting with COP1 and repressing HY5 transcriptional activity

Grapevine Biotechnology: Molecular Approaches Underlying Abiotic and Biotic Stress Responses

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