Interaction of carboxylated CdSe/ZnS quantum dots with fish embryos: Towards understanding of nanoparticles toxicity

Rotomskis, Ričardas; Jurgelėnė, Živilė; Stankevičius, Mantas; Stankevičiūtė, Milda; Kazlauskienė, Nijolė; Jokšas, Kęstutis; Montvydienė, Danguolė; Kulvietis, Vytautas; Karabanovas, Vitalijus

doi:10.1016/j.scitotenv.2018.04.206

Cited by 33 publications

(18 citation statements)

References 64 publications

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“…QDs can induce persistent inflammation and lung dysfunction in the lung, or damage the liver morphology, and lead to centrilobular fibrosis and oxidative stress in the liver (Haque et al, 2013; Roberts et al, 2013). QDs may also affect embryonic development in zebrafish models (Rotomskis et al, 2018). QDs based on CdSe are one of the most classical, common, and widely used at present.…”

Section: Discussionmentioning

confidence: 99%

“…In this regard, some in vivo studies have reported several effects of exposure of QDs (Xu et al, 2016). Rotomskis et al reported that carbonylated CdSe/ZnS QDs could cause the fish embryos damage and induce the development of fish (Rotomskis et al, 2018). Zhang et al reported that CdSe QDs could damage the placenta and lead to fetus malformation though the QDs were effectively blocked by the placental barrier (Zhang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Cardiotoxicity of Intravenously Administered CdSe/ZnS Quantum Dots in BALB/c Mice

Tian

Wang

et al. 2019

Front. Pharmacol.

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Since CdSe quantum dots (QDs) are increasingly used in electronics, medical, and pharmaceutical science due to their excellent optical properties, it is necessary to carry out thorough and systematic studies on their biosafety. Numerous studies have reported the toxicity of QDs on liver, kidney, immune system, and reproductive system. However, few studies have been done on the cardiotoxicity of QDs. In this study, we administered carboxylated CdSe/ZnS QDs in BALB/c mice via the tail vein and analyzed the in vivo cardiotoxicity of CdSe/ZnS QDs. The body weight, hematology, serum biochemistry, histology, heart elements concentration, echocardiography, and heart oxidative stress markers were carried out at different time. There were no significant differences in body weight and heart organ index between QDs group and the control group. Hematology results showed the platelet (PLT) counts on Day 1 and Day 42 in both high dose QDs group and low dose QDs group, and the PLT counts on Day1 in the high dose group were significantly higher than that in control group. Serum biochemistry results showed that lactate dehydrogenase (LDH), creatine kinase (CK), and creatine kinase isoenzyme (CK-MB) of mice exposed to CdSe/ZnS QDs were significantly higher than that of the control group on Day 1, and CK-MB levels still remained high on Day 7. A higher concentration of Cd was observed in the heart of CdSe/ZnS QDs exposed mice on Day 42, whereas no Cd was detected in the control group, which suggested that QDs can accumulate in heart. No significant histopathological changes and cardiac function were observed in all mice at different time after treatment. Increased level of glutathione peroxidase (GPx) and malondialdehyde (MDA) was observed in mice administered with high dose QDs on Day 1, and increased level of total antioxidant capacity (T-AOC) and MDA activities was observed on Day 42. These results indicated that CdSe/ZnS QDs could accumulate in heart, cause some biochemical indicators change, induce oxidative damage, and have cardiotoxicity. Our findings might provide valuable information on the biological safety evaluation of the cardiovascular system of QDs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cardiotoxicity of Intravenously Administered CdSe/ZnS Quantum Dots in BALB/c Mice

Tian

Wang

et al. 2019

Front. Pharmacol.

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“…They are amphiphilic polymer coated CdSe/ZnS QDs with carboxyl groups, average hydrodynamic diameter of 14.2 nm and zeta potential − 32.97 mV. A layer covering QDs allows facile dispersion of the quantum dots in aqueous solutions with retention of their optical properties [71]. For more physicochemical characteristics of QDs, view supplementary information (Additional file 5).…”

Section: Methodsmentioning

confidence: 99%

Uptake and distribution of carboxylated quantum dots in human mesenchymal stem cells: cell growing density matters

et al. 2019

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BackgroundHuman mesenchymal stem cells (MSCs) have drawn much attention in the field of regenerative medicine for their immunomodulatory and anti-inflammatory effects. MSCs possess specific tumor-oriented migration and incorporation highlighting the potential for MSCs to be used as an ideal carrier for anticancer agents. Bone marrow is the main source of MSCs for clinical applications. MSCs tracking in vivo is a critical component of the safety and efficacy evaluation of therapeutic cell products; therefore, cells must be labeled with contrast agents to enable visualization of the MSCs migration in vivo. Due to their unique properties, quantum dots (QDs) are emerging as optimal tools in long-term MSC optical imaging applications. The aim of this study was to investigate the uptake dynamics, cytotoxity, subcellular and extracellular distribution of non-targeted carboxylated quantum dots in human bone marrow MSCs at different cell growing densities.ResultsQDs had no negative impact on MSC viability throughout the experiment and accumulated in all observed cells efficiently; however, in some MSCs QDs induced formation of lipid droplets. At low cell growing densities QDs distribute within MSCs cytoplasm already after 1 h of incubation reaching saturation after 6 h. After 24 h QDs localize mainly in the perinuclear region of the cells in endosomes. Interestingly, in more confluent culture QDs localize mostly outside MSCs. QDs abundantly mark MSC long filopodia-like structures attaching neighboring cells. At high cell density cultivation, we for the first time demonstrated that carboxylated QDs localize in human bone marrow MSC extracellular matrix. Moreover, we observed that average photoluminescence lifetime of QDs distributed in extracellular matrix are longer than lifetimes of QDs entrapped in endocytic vesicles; thus, for the first time showing the possibility to identify and distinguish localization of QDs in various extracellular and intracellular structures using fluorescence-lifetime imaging microscopy without additional staining assays.ConclusionCarboxylated QDs can be used as nonspecific and effective dye for staining of human bone marrow MSCs and their specific extracellular structures. These results are promising in fundamental stem cell biology as well as in cellular therapy, anticancer drug delivery and tissue engineering.Electronic supplementary materialThe online version of this article (10.1186/s12951-019-0470-6) contains supplementary material, which is available to authorized users.

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“…In the case of small particles, dilution expectations are not always met. Carboxilated CdSe/ZnS QDs can agglomerate in deep well water and on the surface of fish embryos (Rotomskis et al, 2018). The damaging potential of an ENM is inherent to the particle, but modifications can be done to decrease or delay it.…”

Section: Cadmium Nanoparticlesmentioning

confidence: 99%

Toxicology of Nanomaterials on Zebrafish

León¹,

Cotto²,

Márquez³

2019

American Journal of Engineering and Applied Sciences

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Increases in the use and the progressive manufacture of new Engineered Nanomaterials (ENMs) lead to inquire about their impact on the environment. Due to the small size, high reactivity inside organisms and the unusual physicochemical properties of the ENMs, the predictions of toxicity are very complex. The zebrafish (Danio rerio) has been granted as a practical alternative to study the toxicity of ENMs. In this article the toxic effects of silver nanoparticles, titanium oxide nanoparticles, zinc oxide nanoparticles, carbon nanotubes, copper nanoparticles, gold nanoparticles, cadmium nanoparticles and nano plastics were reviewed trough the most recent literature available. Every ENMs should be studied in depth independently, considering co-exposures, environmental matrices, the effects of variations in size and concentrations, the potential effects of prolonged exposure, the coverage of ENMs, specific organism and targeted organs. This information will help to identify deficiencies in research trends and reinforce the safest ways to use ENMs.

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Interaction of carboxylated CdSe/ZnS quantum dots with fish embryos: Towards understanding of nanoparticles toxicity

Cited by 33 publications

References 64 publications

Cardiotoxicity of Intravenously Administered CdSe/ZnS Quantum Dots in BALB/c Mice

Cardiotoxicity of Intravenously Administered CdSe/ZnS Quantum Dots in BALB/c Mice

Uptake and distribution of carboxylated quantum dots in human mesenchymal stem cells: cell growing density matters

Toxicology of Nanomaterials on Zebrafish

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