Interaction of bifidobacterial lipoteichoic acid with human intestinal epithelial cells

Camp, H J Op den; Oosterhof, Arie; Veerkamp, J.H.

doi:10.1128/iai.47.1.332-334.1985

Cited by 53 publications

(12 citation statements)

References 27 publications

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“…A strong inhibition of adhesion was observed with purified LTA as well as with La1 SCS, indicating that LTA plays a role in the mechanism of adhesion of L. johnsonii. Indeed, studies with staphylococci (51), streptococci (3,5,41,52), Bifidobacterium bifidus (42), and lactobacilli (11,47) have shown that LTA can inhibit their adhesion to epithelial cells. Binding of LTA to mammalian membranes has already been described for group A streptococci (6), in which LTA is anchored to a protein on the surface of the bacterial cells and interacts through its lipid moiety with fibronectin molecules deposited on and bound to the epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

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Cell Surface-Associated Lipoteichoic Acid Acts as an Adhesion Factor for Attachment of Lactobacillus johnsonii La1 to Human Enterocyte-Like Caco-2 Cells

Granato

Perotti

Masserey

et al. 1999

Appl Environ Microbiol

214

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The influence of pH on the adhesion of twoLactobacillus strains to Caco-2 human intestinal cells was investigated. One strain, Lactobacillus johnsonii La1, was adherent at any pH between 4 and 7. The other one, L. acidophilus La10, did not attach to this cell line under the same experimental conditions. On the basis of these results, we used the monoclonal antibody technique as a tool to determine differences on the surface of these bacteria and to identify a factor for adhesion. Mice were immunized with live La1, and the hybridomas produced by fusion of spleen cells with ONS1 cells were screened for the production of antibodies specific for L. johnsonii La1. A set of these monoclonal antibodies was directed against a nonproteinaceous component of the L. johnsonii La1 surface. It was identified as lipoteichoic acid (LTA). This molecule was isolated, chemically characterized, and tested in adhesion experiments in the same system. The adhesion of L. johnsonii La1 to Caco-2 cells was inhibited in a concentration-dependent way by purified LTA as well as by L. johnsonii La1 culture supernatant that contained LTA. These results showed that the mechanism of adhesion of L. johnsonii La1 to human Caco-2 cells involves LTA.

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Section: Discussionmentioning

confidence: 99%

“…The importance of lipoteichoic acid (LTA) as a mediator of the adhesion of Lactobacillus spp. or other bacteria to human epithelial cells also has been demonstrated (5,11,41,42,47,51,52). The adhesion of Lactobacillus spp.…”

mentioning

confidence: 91%

Cell Surface-Associated Lipoteichoic Acid Acts as an Adhesion Factor for Attachment of Lactobacillus johnsonii La1 to Human Enterocyte-Like Caco-2 Cells

Granato

Perotti

Masserey

et al. 1999

Appl Environ Microbiol

214

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“…L. bulgaricus will establish colonies which are attached to the gut mucosal epithelium cells in germ-free mice [9]. The lipoteichoic acid of Bifidobacterium bifidum subsp, pennsylvanium adheres to human colonic epithelial cells [10]. Lin and Savage [11] suggested that host-specific cryptic plasmids may be involved in adhesion.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic role of dietary lactobacilli and lactobacillic fermented dairy products

Fernandes

Shahani

Amer³

1987

FEMS Microbiology Letters

198

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Lactic acid bacteria present in the human and animal gut are introduced through fermented milk products, food and feed supplements containing viable bacteria. Lactobacillus species, such as L. acidophilus, L. bulgaricus, L. lactis along with B. bifidum and Streptococcus faecium constitute an integral part of the healthy gastro‐intestinal microecology and are involved in the host metabolism. They impart nutritional and therapeutic benefits to the consumer. The vitamins and enzymes produced by the lactic acid bacteria contribute to host metabolism. The antimicrobial substances produced by these bacteria control the proliferation of undesired pathogens. They also elaborate enzymes that aid host metabolism. This is particularly true in case of lactase nonpersistent individuals. Their anticholesteremic properties assist in lowering serum cholesterol. It has been suggested that the tumor suppression trait of these microbes reduces the incidence of colon cancer.

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“…Several Lactobacillus species possess a surface layer protein comprising glyco-proteins (Vadillo-Rodrı guez et al, 2004), the S-proteins with relative molecular weight between 40,000 and 200,000 (Sara and Sleytr, 2000), which help Lactobacillus to adhere to hexadecane via hydrophobic interactions (Greene and Klaenhammer, 1994;van der Mei et al, 2003). In addition, the presence of predominant apolar groups of bacterial membrane such as saturated fatty acids, monoenoic acids (Veerkamp, 1971), and lipoteichoic acids of bifidobacterial membrane (Op-den-Camp et al, 1985) may also support the adherence.…”

Section: Stability Of Probiotic Bacteria In the Gutmentioning

confidence: 99%

Survival of Microencapsulated Probiotic Bacteria after Processing and during Storage: A Review

Dianawati

Mishra

Shah

2015

Critical Reviews in Food Science and Nutrition

118

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The use of live probiotic bacteria as food supplement has become popular. Capability of probiotic bacteria to be kept at room temperature becomes necessary for customer's convenience and manufacturer's cost reduction. Hence, production of dried form of probiotic bacteria is important. Two common drying methods commonly used for microencapsulation are freeze drying and spray drying. In spite of their benefits, both methods have adverse effects on cell membrane integrity and protein structures resulting in decrease in bacterial viability. Microencapsulation of probiotic bacteria has been a promising technology to ensure bacterial stability during the drying process and to preserve their viability during storage without significantly losing their functional properties such acid tolerance, bile tolerance, surface hydrophobicity, and enzyme activities. Storage at room temperatures instead of freezing or low temperature storage is preferable for minimizing costs of handling, transportation, and storage. Concepts of water activity and glass transition become important in terms of determination of bacterial survival during the storage. The effectiveness of microencapsulation is also affected by microcapsule materials. Carbohydrate- and protein-based microencapsulants and their combination are discussed in terms of their protecting effect on probiotic bacteria during dehydration, during exposure to harsh gastrointestinal transit and small intestine transit and during storage.

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Interaction of bifidobacterial lipoteichoic acid with human intestinal epithelial cells

Cited by 53 publications

References 27 publications

Cell Surface-Associated Lipoteichoic Acid Acts as an Adhesion Factor for Attachment of Lactobacillus johnsonii La1 to Human Enterocyte-Like Caco-2 Cells

Cell Surface-Associated Lipoteichoic Acid Acts as an Adhesion Factor for Attachment of Lactobacillus johnsonii La1 to Human Enterocyte-Like Caco-2 Cells

Therapeutic role of dietary lactobacilli and lactobacillic fermented dairy products

Survival of Microencapsulated Probiotic Bacteria after Processing and during Storage: A Review

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