Cell Surface-Associated Lipoteichoic Acid Acts as an Adhesion Factor for Attachment of
            <i>Lactobacillus johnsonii</i>
            La1 to Human Enterocyte-Like Caco-2 Cells

Granato, Dominique; Perotti, Fabienne; Masserey, Isabelle; Rouvet, Martine; Golliard, Mireille; Servin, Alain L.; Brassart, Dominique

doi:10.1128/aem.65.3.1071-1077.1999

Cited by 214 publications

(80 citation statements)

References 62 publications

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“…It is likely that the complex interactions between host, pathogen and probiotic are multifactorial and that S-layer proteins contribute to part of the process. Cell wall components such as lipoteichoic acids (Granato et al, 1999), cell wall proteins (Henriksson et al, 1991;Rojas et al, 2002) and other adhesins (Pretzer et al, 2005) all may be important in bacterial adherence to host tissues. Previous attempts at creating Slp null mutants have been difficult (Buck et al, 2005); therefore, addition of S-layer protein extract provides a useful alternate method to determine its role in vitro.…”

Section: Discussionmentioning

confidence: 99%

Surface-layer protein extracts from Lactobacillus helveticus inhibit enterohaemorrhagic Escherichia coli O157:H7 adhesion to epithelial cells

Johnson‐Henry¹,

Hagen²,

Gordonpour³

et al. 2007

Cell Microbiol

229

125

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SummaryAdherence of intestinal pathogens, including Escherichia coli O157:H7, to human intestinal epithelial cells is a key step in pathogenesis. Probiotic bacteria, including Lactobacillus helveticus R0052 inhibit the adhesion of E. coli O157:H7 to epithelial cells, a process which may be related to specific components of the bacterial surface. Surface-layer proteins (Slps) are located in a paracrystalline layer outside the bacterial cell wall and are thought to play a role in tissue adherence. However, the ability of S-layer protein extract derived from probiotic bacteria to block adherence of enteric pathogens has not been investigated. Human epithelial (HEp-2 and T84) cells were treated with S-layer protein extract alone, infected with E. coli O157:H7, or pretreated with S-layer protein extract prior to infection to determine their importance in the inhibition of pathogen adherence. The effects of S-layer protein extracts were characterized by phasecontrast and immunofluorescence microscopy and measurement of the transepithelial electrical resistance of polarized monolayers. Pre-treatment of host epithelial cells with S-layer protein extracts prior to E. coli O157:H7 infection decreased pathogen adherence and attaching-effacing lesions in addition to preserving the barrier function of monolayers. These in vitro studies indicate that a non-viable constituent derived from a probiotic strain may prove effective in interrupting the infectious process of an intestinal pathogen.

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Section: Discussionmentioning

confidence: 99%

Surface-layer protein extracts from Lactobacillus helveticus inhibit enterohaemorrhagic Escherichia coli O157:H7 adhesion to epithelial cells

Johnson‐Henry¹,

Hagen²,

Gordonpour³

et al. 2007

Cell Microbiol

229

125

View full text Add to dashboard Cite

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“…Lipoteichoic acid has been identified as a factor responsible for the adhesion of the L. johnsonii La1 bacteria. This molecule has been isolated from the bacterial cell wall and from the culture supernatant, and is responsible for the concentration-dependent inhibition of La1 adhesion to Caco-2 cells [128]. L. animalis and L. fermentum bacteria have lectin-like protein structures on their surfaces, and L. animalis bacteria has been shown to have ribitol teichoic acids in its cell wall [129].…”

Section: Adhesiveness Propertiesmentioning

confidence: 99%

Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens

2004

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The gastrointestinal tract is a complex ecosystem that associates a resident microbiota and cells of various phenotypes lining the epithelial wall expressing complex metabolic activities. The resident microbiota in the digestive tract is a heterogeneous microbial ecosystem containing up to 1 x 10(14) colony-forming units (CFUs) of bacteria. The intestinal microbiota plays an important role in normal gut function and maintaining host health. The host is protected from attack by potentially harmful microbial microorganisms by the physical and chemical barriers created by the gastrointestinal epithelium. The cells lining the gastrointestinal epithelium and the resident microbiota are two partners that properly and/or synergistically function to promote an efficient host system of defence. The gastrointestinal cells that make up the epithelium, provide a physical barrier that protects the host against the unwanted intrusion of microorganisms into the gastrointestinal microbiota, and against the penetration of harmful microorganisms which usurp the cellular molecules and signalling pathways of the host to become pathogenic. One of the basic physiological functions of the resident microbiota is that it functions as a microbial barrier against microbial pathogens. The mechanisms by which the species of the microbiota exert this barrier effect remain largely to be determined. There is increasing evidence that lactobacilli and bifidobacteria, which inhabit the gastrointestinal microbiota, develop antimicrobial activities that participate in the host's gastrointestinal system of defence. The objective of this review is to analyze the in vitro and in vivo experimental and clinical studies in which the antimicrobial activities of selected lactobacilli and bifidobacteria strains have been documented.

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“…Several Lactobacillus extracellular compounds described as important for adhesion appear to contribute in a rather unspecific manner. For example, LTA provides the main component of hydrophobicity to the Lactobacillus cell envelope, which appears to be the reason for its involvement in the adhesive characteristics of L. johnsonii (Granato et al, 1999), L. rhamnosus (Lebeer et al, 2007) and L. reuteri (Walter et al, 2007). Similarly, exopolysaccharides, which contributes to cell-surface physiochemical properties and shielding of other cell-surface adhesins, is important for the adhesive characteristics of L. acidophilus (Lorca et al, 2002) and L. rhamnosus (Ruas-Madiedo et al, 2006).…”

Section: Adhesion To Mucus and Host Mucosamentioning

confidence: 99%

The extracellular biology of the lactobacilli

et al. 2010

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Lactobacilli belong to the lactic acid bacteria, which play a key role in industrial and artisan food raw-material fermentation, including a large variety of fermented dairy products. Next to their role in fermentation processes, specific strains of Lactobacillus are currently marketed as health-promoting cultures or probiotics. The last decade has witnessed the completion of a large number of Lactobacillus genome sequences, including the genome sequences of some of the probiotic species and strains. This development opens avenues to unravel the Lactobacillus-associated health-promoting activity at the molecular level. It is generally considered likely that an important part of the Lactobacillus effector molecules that participate in the proposed health-promoting interactions with the host (intestinal) system resides in the bacterial cell envelope. For this reason, it is important to accurately predict the Lactobacillus exoproteomes. Extensive annotation of these exoproteomes, combined with comparative analysis of species- or strain-specific exoproteomes, may identify candidate effector molecules, which may support specific effects on host physiology associated with particular Lactobacillus strains. Candidate health-promoting effector molecules of lactobacilli can then be validated via mutant approaches, which will allow for improved strain selection procedures, improved product quality control criteria and molecular science-based health claims.

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Cell Surface-Associated Lipoteichoic Acid Acts as an Adhesion Factor for Attachment of Lactobacillus johnsonii La1 to Human Enterocyte-Like Caco-2 Cells

Cited by 214 publications

References 62 publications

Surface-layer protein extracts from Lactobacillus helveticus inhibit enterohaemorrhagic Escherichia coli O157:H7 adhesion to epithelial cells

Surface-layer protein extracts from Lactobacillus helveticus inhibit enterohaemorrhagic Escherichia coli O157:H7 adhesion to epithelial cells

Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens

The extracellular biology of the lactobacilli

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