Interaction of antioxidant flavonoids with calf thymus DNA analyzed by spectroscopic and electrochemical methods

Synthesis, DNA-Binding and Antibacterial Activity of the Cell-Penetrating Peptide HIV-1 Tat (49-57)

“…The intercalative binding mode can stabilize the helix structure of DNA and causes the melting temperature to increase by about 5-8°, but the electrostatic and groove binding interactions cause no obvious or minimal change in T m [15,24] . The melting curves ( fig.…”

“…The specific CD spectrum of B-form DNA consists of a negative band at 247 nm caused by the right-handed helicity of DNA and a positive band at 275 nm caused by the accumulation of base pairs [15] . The CD spectra of ct-DNA with Tat (49-57) are shown in fig.…”

Interactions of an anticancer drug lomustine with single and double stranded DNA at physiological conditions analyzed by electrochemical and spectroscopic methods

“…and SWV were performed using an EG&G PAR Model 263A Potentiostat / Galvanostat controlled by an IBM microcomputer with EG&G PAR M 270 software in conjunction with a PAR Model 303A hanging mercury drop electrode (HMDE).native double stranded DNA in water bath at a 100 °C for 30 min, followed by promptly cooling in ice bath[21]. The formation of ssDNA from dsDNA is checked by gel electrophoresis measurements.…”

Interactions of an anticancer drug Formestane with single and double stranded DNA at physiological conditions

“…The concentration of the stock solution of dsDNA (4.1x10 -3 M in nucleotide phosphate, NP) was determined by UV absorbance at 260 nm using the molar extinction coefficient (ε) at 6600 M -1 cm -1 . Denatured single stranded DNA (ssDNA) was prepared by heating native double stranded DNA in water bath at a 100 °C for 30 min, followed by promptly cooling in ice bath [30]. The formation of ssDNA from dsDNA is checked by gel electrophoresis measurements.…”

“…In recent years, electrochemical methods coupled with spectroscopy techniques could provide a relatively easy way to obtain useful insights into the molecular mechanism of drug-DNA interaction, which could be an important step in the development of a new anticancer drug to deal with genetic binding [24][25][26][27].…”