Interaction of an ordered mesoporous bioactive glass with osteoblasts, fibroblasts and lymphocytes, demonstrating its biocompatibility as a potential bone graft material

Alcaide, María José; Portolés, Pilar; López-Noriega, Adolfo; Arcos, Daniel; Vallet‐Regí, María; Portolés, María Teresa

doi:10.1016/j.actbio.2009.09.008

Cited by 110 publications

(69 citation statements)

References 36 publications

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“…As it has been indicated above, different cellular responses are obtained depending on each biomaterial and depending on each cell type (references [37][38][39] …”

Section: Authorsmentioning

confidence: 99%

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Early in vitro response of macrophages and T lymphocytes to nanocrystalline hydroxyapatites

Matesanz

Feito

Oñaderra

et al. 2014

Journal of Colloid and Interface Science

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“…As it has been indicated above, different cellular responses are obtained depending on each biomaterial and depending on each cell type (references [37][38][39] …”

Section: Authorsmentioning

confidence: 99%

“…In conclusion, the effects observed in the present study with nano-HA and nano-SiHA on macrophages and lymphocytes are characteristic for these materials and for the cell types used in this study. All these references (37)(38)(39) …”

mentioning

confidence: 99%

Early in vitro response of macrophages and T lymphocytes to nanocrystalline hydroxyapatites

Matesanz

Feito

Oñaderra

et al. 2014

Journal of Colloid and Interface Science

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“…9,39 On the other hand, ion-induced cell death such as that from excessive Ca 2+ may account for lower cell viability resulting from the initial ion release from the composite scaffolds. 40,41 Collagen-based materials that serve as analogs of organic components of bone matrix have previously been developed for bone regeneration. 10,42 It was hypothesized that the incorporation of MHMs or Zn-MHMs would improve the osteoinductive potential of the Coll scaffolds.…”

Section: Yu Et Almentioning

confidence: 99%

Evaluation of zinc-doped mesoporous hydroxyapatite microspheres for the construction of a novel biomimetic scaffold optimized for bone augmentation

Sun

et al. 2017

IJN

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Biomaterials with high osteogenic activity are desirable for sufficient healing of bone defects resulting from trauma, tumor, infection, and congenital abnormalities. Synthetic materials mimicking the structure and composition of human trabecular bone are of considerable potential in bone augmentation. In the present study, a zinc (Zn)-doped mesoporous hydroxyapatite microspheres (Zn-MHMs)/collagen scaffold (Zn-MHMs/Coll) was developed through a lyophilization fabrication process and designed to mimic the trabecular bone. The Zn-MHMs were synthesized through a microwave-hydrothermal method by using creatine phosphate as an organic phosphorus source. Zn-MHMs that consist of hydroxyapatite nanosheets showed relatively uniform spherical morphology, mesoporous hollow structure, high specific surface area, and homogeneous Zn distribution. They were additionally investigated as a drug nanocarrier, which was efficient in drug delivery and presented a pH-responsive drug release behavior. Furthermore, they were incorporated into the collagen matrix to construct a biomimetic scaffold optimized for bone tissue regeneration. The Zn-MHMs/Coll scaffolds showed an interconnected pore structure in the range of 100–300 μm and a sustained release of Zn ions. More importantly, the Zn-MHMs/Coll scaffolds could enhance the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells. Finally, the bone defect repair results of critical-sized femoral condyle defect rat model demonstrated that the Zn-MHMs/Coll scaffolds could enhance bone regeneration compared with the Coll or MHMs/Coll scaffolds. The results suggest that the biomimetic Zn-MHMs/Coll scaffolds may be of enormous potential in bone repair and regeneration.

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“…10 6 cells were seeded onto scaffolds in P-48 culture plates and cultured 3d in the presence of Y-CD3-1 (anti-CD3 antibody, 5 µg/mL) or LPS (50 µg/ml) or culture medium, in 1 ml final volume. After this time, cells were collected and processed for intracellular cytokine detection by Fluorescenceactivated cell sorting (FACS).…”

Section: Analysis Of Intracellular Cytokine Expressionmentioning

confidence: 99%

“…9 The response of L929 fibroblasts and Saos-2 osteoblasts to this powdered nanocomposite has been previously studied, demonstrating a significant improved biocompatibility in comparison with conventional mesoporous glasses. 9,10 Furthermore, recently 3D scaffolds based on this nanocomposite material have been exhibited a good preosteoblast cell adhesion, colonization, proliferation and differentiation, suggesting their great potential application in bone tissue engineering. 11 The first characteristic of any scaffold for tissue engineering is that it must be biocompatible, allowing the cell adhesion, proliferation and migration through it.…”

Section: Introductionmentioning

confidence: 99%

Effects of 3D nanocomposite bioceramic scaffolds on the immune response

et al. 2014

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The interaction of new nanocomposite mesoporous glass/hydroxyapatite (MGHA) scaffolds with immune cells involved in both innate and acquired immunity has been studied in vitro as an essential aspect of their biocompatibility assessment. Since the immune response can be affected by the degradation products of bioresorbable scaffolds and scaffold surface changes, both processes have been evaluated.No alterations in proliferation and viability of RAW-264.7 macrophage-like cells were detected after culture on MGHA scaffolds which did not induce cell apoptosis. However, a slight cell size decrease and an intracellular calcium content increase were observed after contact of this cell line with MGHA scaffolds or their extracts. Although no changes in the percentages of RAW cells with low and high contents of reactive oxygen species (ROS) are observed by the treatment with 7 day extracts, this study has revealed modifications of these percentages after direct contact with scaffolds and by the treatment with 24 h extracts, related to the high reactivity/bioactivity of this MGHA nanocomposite at initial times. Furthermore, when normal fresh murine spleen cells were used as an experimental model closer to physiological conditions, no significant alterations in the activation of different immune cell subpopulations were detected in the presence of 24 h MGHA extract. MGHA scaffolds did not affect either the spontaneous apoptosis or intracellular cytokine expression (IL-2, IL-10, IFN-g, and TNF-a)after 24 h treatment. The results obtained in the present study with murine immune cell subpopulations (macrophages, lymphocytes B, lymphocytes T and natural killer cells) support the biocompatibility of the MGHA material and suggest an adequate host tissue response to their scaffolds upon their implantation.

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Interaction of an ordered mesoporous bioactive glass with osteoblasts, fibroblasts and lymphocytes, demonstrating its biocompatibility as a potential bone graft material

Cited by 110 publications

References 36 publications

Early in vitro response of macrophages and T lymphocytes to nanocrystalline hydroxyapatites

Early in vitro response of macrophages and T lymphocytes to nanocrystalline hydroxyapatites

Evaluation of zinc-doped mesoporous hydroxyapatite microspheres for the construction of a novel biomimetic scaffold optimized for bone augmentation

Effects of 3D nanocomposite bioceramic scaffolds on the immune response

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