Interaction of Amine Oxides and Quaternary Ammonium Salts With Membrane and Membrane-Associated Processes in E. Coli Cells: Mode of Action

Kopecka-Leitmanová, Anna; Devı́nsky, Ferdinand; Mlynarcík, D; Lacko, I.

doi:10.1515/dmdi.1989.7.1.29

Cited by 33 publications

(14 citation statements)

References 13 publications

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“…Leakage of K + is the first indication of membrane damage in micro‐organisms (Lambert and Hammond 1973). Our studies support the previous work (Kopecka‐Leitmanova et al . 1989; Takasaki et al .…”

Section: Discussionsupporting

confidence: 93%

See 1 more Smart Citation

Activity and mechanisms of action of selected biocidal agents on Gram-positive and -negative bacteria

Walsh¹,

Maillard²,

Russell³

et al. 2003

J Appl Microbiol

440

249

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Aims: This study investigates the antimicrobial activity and mode of action of two natural products, eugenol and thymol, a commonly utilized biostatic agent, triclocarban (TCC), and two surfactants, didecyldimethylammonium chloride (DDDMAC) and C 10 -C 16 alkyldimethyl amine N-oxides (ADMAO). Methods and Results: Methods used included: determination of minimum inhibitory concentrations (MICs), lethal effect studies with suspension tests and the investigation of sub-MIC concentrations on growth of E. coli, Staph. aureus and Ps. aeruginosa using a Bioscreen microbiological analyser. Leakage of intracellular constituents and the effects of potentiating agents were also investigated. Only DDDMAC was bactericidal against all of the organisms tested. Eugenol, thymol and ADMAO showed bacteriostatic and bactericidal activity, but not against Ps. aeruginosa. TCC was only bacteristatic against Staph. aureus, but like the other agents, it did affect the growth of the other organisms in the Bioscreen experiments. All of the antimicrobial agents tested were potentiated by the permeabilizers to some extent and leakage of potassium was seen with all of the agents except TCC. Conclusions: DDDMAC was bactericidal against all organisms tested and all compounds had some bacteriostatic action. Low level static effects on bacterial growth were seen with sub-MIC concentrations. Membrane damage may account for at least part of the mode of action of thymol, eugenol, DDDMAC and ADMAO. Significance and Impact of the Study: The ingredients evaluated demonstrated a range of bactericidal and bacteriostatic properties against the Gram-negative and -positive organisms evaluated and the membrane (leakage of intracellular components) was implicated in the mode of action for most (except TCC). Sub-MIC levels of all ingredients did induce subtle effects on the organisms which impacted bacterial growth, even for those which had no true inhibitory effects.

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Section: Discussionsupporting

confidence: 93%

“…The activity of ADMAOs increases with chain length up to a cut‐off point of 14 (Birnie et al . 2000) and is thought to be linked to interaction with the cell membrane (Kopecká‐Leitmanová et al 1989).…”

Section: Introductionmentioning

confidence: 99%

Activity and mechanisms of action of selected biocidal agents on Gram-positive and -negative bacteria

Walsh¹,

Maillard²,

Russell³

et al. 2003

J Appl Microbiol

440

249

View full text Add to dashboard Cite

show abstract

“…Amine oxide (AO; C 10 ‐C 16 ‐alkyldimethyl N‐oxides) is an amphoteric surfactant, in the form of a mixture with differing chain lengths, which is widely used in cleaning and personal care products, usually in conjunction with other surfactants. The bacteriostatic and bactericidal activities of AO have been reported previously, together with its potential for use in oral hygiene (14–19). In this study, we report the use of a biofilm‐based efficacy protocol to test in vitro the activity of different treatment regimens of AO against biofilms of Streptococcus mutans grown on hydroxyapatite (HA) disks in a saliva‐based medium.…”

mentioning

confidence: 74%

A simulated oral hygiene model to determine the efficacy of repeated exposure of amine oxide on the viability of Streptococcus mutans biofilms

Fraud

Maillard

Denyer

et al. 2007

European J Oral Sciences

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The use of biocide in oral care products is important for controlling microbial pathogens. However, the use of biofilm tests that investigate repeated exposure to biocide, to mimic in situ treatment, has rarely been reported in the literature. The present study describes the application of a biofilm-based efficacy protocol, for testing the effect of repeated exposure to antimicrobials on biofilm, in an attempt to mimic oral care regimens. The activity of different treatment regimens, including repeated exposure to amine oxide (AO; C(10)-C(16)-alkyldimethyl N-oxides; 1.1% v/v), was conducted against 16-h Streptococcus mutans biofilms grown on hydroxyapatite disks. Single exposure to AO alone produced a 3 log(10) reduction in microbial count, but when combined with mechanical removal, a 5 log(10) reduction in microbial count was observed. Treatments incorporating repeated exposure to AO reduced the microbial count below the level of detection, even when exposure to AO was interspersed with recovery periods. The presence of organic load produced an additional 2 log(10) reduction in the microbial count. This study showed that the application of a biofilm-based efficacy protocol to mimic oral care regimens allowed the reproducible testing of repeated antimicrobial exposures against bacterial biofilm. In addition, AO was confirmed to be an excellent biocide for eliminating S. mutans biofilms and could therefore be beneficial in oral care formulations.

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“…Kopecká-Leitmannová et al 45 found that MIC of C 12 NO for Escherichia coli was 0,29 mM. The range of C 12 NO concentrations, where the membrane perturbation and solubilization was observed for the EYPC model membrane, correspond surprisingly well to these MIC values.…”

Section: Resultsmentioning

confidence: 71%

Cholesterol protects phosphatidylcholine liposomes from N,N-dimethyldodekanamine N-oxide influence

Huláková

Gallová

Devı́nsky

2015

ACSi

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The interaction of N, N-dimethyl-1-dodecanamine N-oxide (C 12 NO) with egg yolk phosphatidylcholine (EYPC) liposomes containing cholesterol (CHOL) was studied. The perturbation of CHOL-EYPC bilayers in unilamellar liposomes (ULL) was observed by the leakage of fluorescence probe calcein. Weak leakage is observed at low surfactant concentration c C12NO (minimal perturbation of the bilayer) followed by an intensive leakage at a middle c C12NO (creation of pores). No change in fluorescence intensity was measured at high c C12NO (calcein totally released from liposomes). The higher CHOL amount in the bilayer, the more surfactant is needed to create pores in the bilayer. Solubilization of CHOL-EYPC ULL induced by C 12 NO was studied turbidimetrically. The solubilization curve consists of three parts: saturation of bilayer at low c C12NO (liposomes are preserved), followed by solubilization (liposome -mixed micelle transition) and post-solubilization. The C 12 NO concentration needed for the onset of the solubilization raise with the increase of n CHOL :n EYPC . The structure of liposomes is still preserved at total calcein release for all n CHOL :n EYPC .

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Interaction of Amine Oxides and Quaternary Ammonium Salts With Membrane and Membrane-Associated Processes in E. Coli Cells: Mode of Action

Cited by 33 publications

References 13 publications

Activity and mechanisms of action of selected biocidal agents on Gram-positive and -negative bacteria

Activity and mechanisms of action of selected biocidal agents on Gram-positive and -negative bacteria

A simulated oral hygiene model to determine the efficacy of repeated exposure of amine oxide on the viability of Streptococcus mutans biofilms

Cholesterol protects phosphatidylcholine liposomes from N,N-dimethyldodekanamine N-oxide influence

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