Interaction of algal polysaccharide with lipid Langmuir monolayers

Brito, Audrey Kalinouski de; Caseli, Luciano; Nordi, Cristina Souza Freire

doi:10.1016/j.msec.2011.09.002

Cited by 14 publications

(11 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Monolayers of DMPC alone or in combination with AgLNPs and the corresponding NPs individual components were prepared to evaluate the DMPC-AgLNPs interactions and the surface activity of the NP constituents. Langmuir films have been used to study in vitro the binding and/or penetration of molecules through model mammalian membranes, in particular for systems affecting the elasticity of the membrane (Pavinatto et al, 2007;De Brito et al, 2011).…”

Section: Interaction Of Aglnps With Mammalian Model Membranementioning

confidence: 99%

Interaction of Silver-Lignin Nanoparticles With Mammalian Mimetic Membranes

Hoyo

Ivanova

Torrent‐Burgués

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Silver nanoparticles (AgNPs) have broad spectrum antibacterial activity, but their toxicity to human cells has raised concerns related to their use as disinfectants or coatings of medically relevant surfaces. To address this issue, NPs comprising intrinsically bactericidal and biocompatible biopolymer and Ag with high antibacterial efficacy against common pathogens and compatibility to human cells have been engineered. However, the reason for their lower toxicity compared to AgNPs has not yet been elucidated. This work studies the in vitro interaction of AgLNPs with model mammalian membranes through two approaches: (i) Langmuir films and (ii) supported planar bilayers studied by quartz crystal microbalance and atomic force spectroscopy. These approaches elucidate the interactions of AgLNPs with the model membranes indicating a prominent effect of the bioresourced lignin to facilitate the binding of AgLNPs to the mammalian membrane, without penetrating through it. This study opens a new avenue for engineering of hybrid antimicrobial biopolymer-Ag or other metal NPs with improved bactericidal effect whereas maintaining good biocompatibility.

show abstract

Section: Interaction Of Aglnps With Mammalian Model Membranementioning

confidence: 99%

Interaction of Silver-Lignin Nanoparticles With Mammalian Mimetic Membranes

Hoyo

Ivanova

Torrent‐Burgués

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Nowotarski et al [113] showed that the electrical profile of the membrane is also relevant More complex materials such as polysaccharide-protein complexes extracted from fungi or algae were made to interact with charged lipids to probe possible therapeutic actions [114,115]. Schmidt et al [114] showed that the very slightly surface active protein- Langmuir monolayers of cholesterol/DPPC = 0.9 [117] or as more complicated ternary and quaternary mixtures [118] with different compositions of its inner and outer leaflets.…”

Section: Other Polysaccharidesmentioning

confidence: 99%

“…Finally, the authors showed that CAb remains at the interface even at large pressures and can be transferred along with DMPA to LB films, similarly to chitosan. Spectroscopic data (PM-IRRAS) for such mixed LB films deposited onto solid substrates indicate that CAb composition is not changed after interaction with DMPA and transfer onto a solid support.PM-IRRAS was also used by De Brito et al[115] to show that polysaccharide extracts from extracellular algal (EPS) interact preferentially with the polar heads of positively-charged phospholipids. However, the same extracts interact with the alkyl chain regions of negativelycharged lipids (mainly through hydrophobic interactions).…”

mentioning

confidence: 99%

Interactions of bioactive molecules & nanomaterials with Langmuir monolayers as cell membrane models

et al. 2015

Self Cite

View full text Add to dashboard Cite

“…An appealing approach for studying the interaction between biological entities and well-defined lipid-based layers is by the Langmuir–Blodgett (LB) technique, in which commonly the Langmuir monolayers mimic membranes and the biological components are injected into the subphase. Using this approach, interactions of extracellular polysaccharides (EPS), hormones, and enzymes with lipid Langmuir monolayers were examined. − Additionally, the Langmuir monolayer of E. coli lipid extract was formed for studying the binding mechanism and oxidation reactions of photosensitizers .…”

Section: Introductionmentioning

confidence: 99%

Interactions of Microorganisms with Lipid Langmuir Layers

et al. 2021

View full text Add to dashboard Cite

Preventing microbial contamination of aquatic environments is crucial for the proper supply of drinking water. Hence, understanding the interactions that govern bacterial and virus adsorption to surfaces is crucial to prevent infection transmittance. Here, we describe a new approach for studying the organization and interactions of various microorganisms, namely, Escherichia coli (E. coli) bacteria, E. coli-specific bacteriophage T4, and plant cucumber green mottle mosaic viruses (CGMMV), at the air/water interface using the Langmuir–Blodgett (LB) technique. CGMMV were found as applicable candidates for further studying their interactions with Langmuir lipid monolayers. The zwitterionic, positively, and negatively charged LB lipid monolayers with adsorbed viruses were deposited onto solid supports and characterized by atomic force microscopy. Using polymerase chain reaction, we indicated that the adsorption of CGMMV onto the LB monolayer is a result of electrostatic interactions. These insights are useful in engineering membrane filters that prevent biofouling for efficient purification systems.

show abstract

Interaction of algal polysaccharide with lipid Langmuir monolayers

Cited by 14 publications

References 29 publications

Interaction of Silver-Lignin Nanoparticles With Mammalian Mimetic Membranes

Interaction of Silver-Lignin Nanoparticles With Mammalian Mimetic Membranes

Interactions of bioactive molecules & nanomaterials with Langmuir monolayers as cell membrane models

Interactions of Microorganisms with Lipid Langmuir Layers

Contact Info

Product

Resources

About