Interaction of Adipose-Derived Stromal Cells with Breast Cancer Cell Lines

Abstract

Background: Assisted lipotransfer for breast reconstruction involves the isolation and supplementation of adipose-derived stromal cells. This procedure has raised concerns regarding safety with respect to promotion of tumor growth and relapse. Several in vitro and animal experimental studies have indicated increased survival, growth, and invasive characteristics of breast cancer cells on interaction with adipose-derived stromal cells. These results seem to be in poor concordance with clinical observations of a low rate of cancer recurrences after assisted lipotransfer. Methods: The authors investigated the effects of adipose-derived stromal cells and adipose-derived stromal cells differentiated into adipocytes and fibroblasts on five breast cancer cell lines (i.e., T47D, MCF-7, BT20, MDA-MB-231, and ZR-75-1) and MCF-10A, a nonmalignant counterpart. Results: Conditioned media of adipose-derived stromal cells stimulated the proliferation of breast cancer cell lines depending on the individual adipose-derived stromal cell–breast cancer cell line combination. Conditioned media of adipose-derived stromal cells differentiated into adipocytes gave a lower response, and conditioned media of fibroblasts were also active. A putative cancer stem cell–like phenotype was not increased by adipose-derived stromal cell–conditioned media, no physical interaction of cancer cells with adipose-derived stromal cells was detectable on scanning electron microscopy, and cell migration was not enhanced. Adipogenic differentiation of adipose-derived stromal cells indicated that hepatocyte growth factor, insulin-like growth factor-binding protein-3, insulin-like growth factor-binding protein-6, interleukin-6, CCL2/MCP-1, and macrophage colony-stimulating factor are not linked to the proliferative activity of conditioned media. Conclusion: The results indicate that the adipose-derived stromal cells used for assisted lipotransfer are not expected to increase the risk of tumor recurrence to a major degree in correspondence with the clinical observation of the affected breast cancer patients. CLINICAL QUESTION/LEVEL OF EVIDENCE: Therapeutic, V.

Effects of Metformin on Bone-derived Mesenchymal Stromal Cell–breast Cancer Cell Line Interactions

“…A fibrinogen matrix stimulates a two-fold increase in BM-MSC cell yield over plastic surfaces without affecting differentiation 52 . The significance of the multitude of adipokines altered in response to metformin is difficult to interpret but increase of osteogenic signals, activation of immune processes and inhibition of MMP9 is expected to be beneficial for patients 53 .…”

Exosomes From Adipose-Derived Stem Cells: The Emerging Roles and Applications in Tissue Regeneration of Plastic and Cosmetic Surgery

“…The exosomes, released from the adipocyte s and ASCs, are emerging as a new type in the crosstalk between breast tumors and adipocytes ( Sauter et al, 2019 ; Teufelsbauer et al, 2019 ). Previously, Lin et al (2013) investigated the effect of ASC-exos on breast cancer MCF7 cells, showing that ASC-exos activated the Wnt and Hh signaling pathways to strengthen tumor cell migration.…”

Fat grafts enriched with adipose-derived stem cells

“…In particular, the possibility cannot be ruled out that ASCs may stimulate dormant breast cancer cells after CAL in patients with a mild deformity after mastectomy for breast malignancy. To date, several studies, including in vitro and in vivo experiments, have investigated this issue and reported markedly controversial findings [50][51][52][53]. A clinical analysis of a large series of autologous fat grafts without supplementation with ASCs for breast reconstruction demonstrated a low complication rate and no evidence of increased oncologic risk [54][55][56][57].…”