Interaction mechanism of three egg protein derived ACE inhibitory tri-peptides and DPPC membrane using FS, FTIR, and DSC studies

Ji, Huizhuo; Zhao, Wenzhu; Yu, Zhipeng

doi:10.1016/j.fochx.2022.100366

Cited by 13 publications

(7 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Then, CP-Ca-US was prepared by ultrasound (20-25 kHz) using an ultrasonic cell disruptor (Scientz-IID, Ningbo Xinzhi Biotechnology Co., Ltd, Ningbo, China). According to previous pre-experiments and literature review, the optimized process parameters included ultrasound power (60-180 W), ultrasound time (20-60 min), CaCl 2 /peptides ratio (1/6-1/2), and pH value (5)(6)(7)(8)(9). To exclude the influence of temperature on the chelation process, the glass bottle was placed in an icewater bath, and the temperature was controlled within a stable range (4.46 ± 0.26 °C) detected by a temperature recorder (L93-1, Hangzhou Loggertech Co., Ltd, Hangzhou, China).…”

Section: Preparation Experiments Of Peptide-calcium Chelate By the Ul...mentioning

confidence: 99%

“…Many peptides can resist the degradation in the gastrointestinal and plasma environment (such as pepsin, trypsin, and plasma protease) and reach integrally their target organs. 6,7 Therefore, peptidecalcium chelates could enhance calcium bioavailability and exert the bioactivity of peptides. 8 Many researchers are focusing on food protein-derived peptide-calcium chelates, such as pig bone, chicken foot, milk, and tilapia bone.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Preparation of a cattle bone collagen peptide–calcium chelate by the ultrasound method and its structural characterization, stability analysis, and bioactivity on MC3T3-E1 cells

Zhang

Wang

et al. 2023

Food Funct.

View full text Add to dashboard Cite

show abstract

Section: Preparation Experiments Of Peptide-calcium Chelate By the Ul...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preparation of a cattle bone collagen peptide–calcium chelate by the ultrasound method and its structural characterization, stability analysis, and bioactivity on MC3T3-E1 cells

Zhang

Wang

et al. 2023

Food Funct.

View full text Add to dashboard Cite

show abstract

“…The peptides were synthesised using the solid-phase process by a previously described method (Yuan et al, 2021;Yu et al, 2021aYu et al, , 2021bJi et al, 2022aJi et al, , 2022b.…”

Section: Peptide Synthesismentioning

confidence: 99%

Colla corii asini–derived peptides as tyrosinase inhibitors: identification, inhibitory activity and molecular mechanism

Zhao

2022

Int J of Food Sci Tech

Self Cite

View full text Add to dashboard Cite

The aim of the present study was to investigate the effect of peptides derived from Colla corii asini on tyrosinase. The sequences of Colla corii asini-derived peptides were identified using mass spectrometry. To characterise the potential peptides with tyrosinase inhibitory activity and clarify their molecular mechanism, both in silico digestion and molecular docking were used. In addition, the tyrosinase inhibitory activities of potential peptides in vitro were also validated. The results showed that Colla corii asini consisted of 472 peptides, and most peptides derived from collagen. Peptides DGGR, DGD, NAGE, LVGE and GSEG were released from the digestion of Colla corii asini-derived peptides and possessed potent inhibitory activity against tyrosinase with IC 50 values of 0.92, 0.50, 0.77, 0.66 and 0.69 mg mL À1 , respectively. The molecular interaction results showed that hydrogen bond and van der Waals interactions are important forces in the interaction of peptides with tyrosinase. Surface forces containing aromatic interaction, hydrogen bond, hydrophilicity and solvent-accessible surface contributed to the interaction between tyrosinase and peptides.

show abstract

“…IRW, 3 ADF, 4 FGR, 5 RVPSL, QIGLF 6,7 and TNIIGR, 8 which have the potential to prevent or treat hypertension. 9 Our previous studies have shown that the egg-white-derived ACE inhibitory peptide RVPSL could significantly lower the blood pressure (BP) of spontaneously hypertensive rats (SHRs) in vivo. 6 However, the potential intervention mechanism of RVPSL has not been clarified.…”

Section: Introductionmentioning

confidence: 99%

“…A large number of egg‐white‐derived ACE inhibitory peptides have been identified, i.e. IRW, 3 ADF, 4 FGR, 5 RVPSL, QIGLF 6,7 and TNIIGR, 8 which have the potential to prevent or treat hypertension 9 . Our previous studies have shown that the egg‐white‐derived ACE inhibitory peptide RVPSL could significantly lower the blood pressure (BP) of spontaneously hypertensive rats (SHRs) in vivo 6 .…”

Section: Introductionmentioning

confidence: 99%

Dissecting the potential mechanism of antihypertensive effects of RVPSL on spontaneously hypertensive rats via widely targeted kidney metabolomics

Wang

et al. 2022

J Sci Food Agric

Self Cite

View full text Add to dashboard Cite

BACKGROUND Our previous study has demonstrated that the egg‐white‐derived peptide RVPSL can lower blood pressure in spontaneously hypertensive rats (SHRs), but its potential action mechanism remains unclear. In this work, the underlying mechanism of the antihypertensive effects of RVPSL in SHRs was elucidated using the widely targeted kidney metabolomics approach. RESULTS Ten SHRs were divided into two groups: SHR‐Untreated group (0.9% saline) and SHR‐RVPSL group (50 mg kg−1 body weight RVPSL) for 4 weeks. After 4 weeks, kidney samples were collected and widely targeted (liquid chromatography–electrospray ionization–tandem mass spectrometry) metabolomics was used to detect metabolites. Fifty‐six biomarkers were identified that may be associated with hypertension. Among them, 17 biomarkers were upregulated and 39 biomarkers were downregulated. The results suggested that eight potential biomarkers were identified in kidney samples: O‐phospho‐l‐serine, tyramine, citric acid, 3‐hydroxybutyrate, O‐acetyl‐l‐serine, 15‐oxo‐5Z,8Z,11Z,13E‐eicosatetraenoic acid (15‐oxoETE), dopaquinone and 3,3′,5‐triiodo‐l‐thyronine. These potential biomarkers mainly involved carbon metabolism, thyroid hormone signaling pathway, tyrosine metabolism and arachidonic acid metabolism. CONCLUSION The study suggested that RVPSL may exert antihypertensive effects through upregulation of O‐phospho‐l‐serine, 3‐hydroxybutyrate and 15‐oxoETE, and downregulation of tyramine, citric acid, O‐acetyl‐l‐serine, 3,3′,5‐triiodo‐l‐thyronine and dopaquinone. The antihypertensive effects of RVPSL may be related to carbon metabolism, thyroid hormone signaling pathway, tyrosine metabolism and arachidonic acid metabolism. RVPSL exhibited a potent antihypertensive effect, and the antihypertensive effects were associated with inhibition of vascular smooth muscle cell proliferation, vascular remodeling, vascular endothelium dysfunction, restoring reactive oxygen species, oxidative stress, inflammation and immune reaction. © 2022 Society of Chemical Industry.

show abstract

Interaction mechanism of three egg protein derived ACE inhibitory tri-peptides and DPPC membrane using FS, FTIR, and DSC studies

Cited by 13 publications

References 31 publications

Preparation of a cattle bone collagen peptide–calcium chelate by the ultrasound method and its structural characterization, stability analysis, and bioactivity on MC3T3-E1 cells

Preparation of a cattle bone collagen peptide–calcium chelate by the ultrasound method and its structural characterization, stability analysis, and bioactivity on MC3T3-E1 cells

Colla corii asini–derived peptides as tyrosinase inhibitors: identification, inhibitory activity and molecular mechanism

Dissecting the potential mechanism of antihypertensive effects of RVPSL on spontaneously hypertensive rats via widely targeted kidney metabolomics

Contact Info

Product

Resources

About