Preparation of a cattle bone collagen peptide–calcium chelate by the ultrasound method and its structural characterization, stability analysis, and bioactivity on MC3T3-E1 cells

Abstract

This study was designed to prepare cattle bone-derived collagen peptides-calcium chelate by ultrasound method (CP-Ca-US), and its structural characterization, stability, and bioactivity on MC3T3-E1 cells were characterized. Single-factor experiments optimized...

Optimisation of recovery protein hydrolysate from chicken bone by steam explosion strategy and its calcium‐binding capacity and potential pro‐osteogenic activity

“…Similar findings from an earlier study suggested that steam explosion might increase the release of protein hydrolysate from cow bone (Zhang et al ., 2020). The tight binding of the protein to hydroxyapatite that forms the bone structure may be destroyed by the steam explosion treatment, which may also cause the collagen superhelix to open up and produce more soluble protein (Zhang et al ., 2023a, 2023b). Only 56.49 ± 0.67% of the protein extracted from the chicken bone using the ultrasound‐assisted enzyme technique was recovered (Dong et al ., 2019).…”

“…Within 15–24 min, possibly because over time, the high‐pressure steam breaks down the collagen structure to produce a low‐molecular‐weight hydrolysate (major factor). In addition to the mechanical damage that the sudden release of steam causes to the bones of chickens, the accidental heated pressure that results from a steam explosion may also induce protein breakdown (Zhang et al ., 2023a, 2023b). The samples generated under the circumstances of 40% filling rate, 1.8 MPa pressure, and 12 min were ultimately chosen for the follow‐up study (calcium‐binding capacity and osteogenic activity) based on the aforementioned research findings and taking into consideration the recovery rate of chicken bone protein hydrolysate.…”

“…Because of their active side chain groups, such as hydroxyl groups, the acidic amino acids Asp and Glu were critical in chelating metal ions. With calcium ions, these side chain groups might form coordination bonds (Zhang et al ., 2023a, 2023b). This was consistent with previous research.…”

“…Furthermore, as compared to the control and peptide groups, the peptides‐calcium chelate treatment group significantly improved the mineralisation activity of MC3T3‐E1 cells, and this effect was dose‐dependent. This was in line with earlier findings (Wu et al ., 2020; Zhang et al ., 2023a, 2023b). This may be because peptides‐calcium chelate markedly raised intracellular calcium ions concentration, improving MC3T3‐E1 cells' mineralisation activity.…”

“…(2014) showed that using a steam explosion to increase the bio‐utilisation of feather meal is a successful strategy. Furthermore, some studies have also shown that different pressures (1.0–1.6 MPa), times (10–30 min), and filling ratios (10%–20%) have significant effects on the protein recovery efficiency of steam explosion (Shen et al ., 2019a, 2019b; Zhang et al ., 2023a, 2023b). However, there has been little research on the effect of steam explosion on protein extraction from animal bone.…”