Interaction Between the Respiratory Burst Activity of Neutrophil Leukocytes and Experimentally Induced Escherichia coli Mastitis in Cows

Heyneman, Roger; Burvenich, Christian; Vercauteren, R.

doi:10.3168/jds.s0022-0302(90)78756-5

Cited by 106 publications

(124 citation statements)

References 12 publications

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“…The CL of blood PMN was calculated with the same formula as for milk PMN, applying the corrections described by Heyneman et al [15] for eosinophils contributing to the ROI production in the samples, considering that the CL response of eosinophils is five times higher than that of PMN.…”

Section: Chemiluminescence Assaymentioning

confidence: 99%

“…Polymorphonuclear leukocytes (PMN) are important components of the cellular host defense in protecting cows against Escherichia coli infection within the mammary gland [5,15,19,21,39]. Acute E. coli mastitis in early lactating cows is sometimes accompanied by severe general clinical symptoms such as fever and a sudden decrease of milk production in non-injected (non-inflamed) quarters [16,24,37].…”

Section: Introductionmentioning

confidence: 99%

“…The intramammary defense against invading pathogens is highly dependent on the rate of PMN diapedesis into the infected quarters [16], their capacity to produce reactive oxygen intermediates (ROI) [15] and the number of circulating PMN prior to infection [15,21,37,39]. Chemiluminescence (CL) can be used to study the oxygen-dependent bactericidal system of PMN [1,9,23,27].…”

Section: Introductionmentioning

confidence: 99%

“…During E. coli mastitis, the ROI production of blood PMN has been observed to decrease [15]. The ROI production of milk PMN during mastitis, however, is not well characterized.…”

Section: Introductionmentioning

confidence: 99%

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Local and systemic effects of endotoxin mastitis on the chemiluminescence of milk and blood neutrophils in dairy cows

et al. 2001

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-The local and systemic effects of intramammary lipopolysaccharide (LPS) injection on the chemiluminescence (CL) of milk and blood polymorphonuclear leukocytes (PMN) were investigated in six healthy early lactation cows. Clinical signs of acute mastitis such as fever, increased heart rate and a decreased milk production were observed in all cows. Before LPS challenge, the CL activity of milk PMN was significantly lower than that of blood PMN (P < 0.01). A significant negative correlation was found between pre-challenge milk and blood PMN CL and, the decreased milk production in unchallenged quarters. The CL activity of milk PMN from LPS-injected quarters increased following LPS challenge, whereas it remained unchanged in control quarters. The CL activity of blood PMN showed a biphasic increase, with two peaks and a valley below pre-challenge CL activity (P < 0.01). At post-challenge hours (PCH) 6 and 12, the CL activity of milk PMN from LPS-injected quarters exceeded that of blood PMN (P < 0.05 and P < 0.001, respectively). The decreased CL activity of blood PMN and the enhanced CL activity of milk PMN during endotoxin-induced mastitis was reflected by changes in the shape of the CL curve. In blood PMN, a decrease of the second peak of the CL curve suggests that the myeloperoxidase (MPO)-H 2 O 2 system is impaired during endotoxin-induced mastitis. In contrast, the MPO-H 2 O 2 system was enhanced in milk PMN from challenged quarters. The highest duration and intensity of reactive oxygen intermediate (ROI) production was observed in milk PMN from LPS-injected quarters at PCH 12. The increased viability of PMN in LPS-injected quarters and to a lesser extent in control quarters suggests possible effects of both facilitated diapedesis and inflammatory mediators on milk PMN survival. In conclusion, our results suggest that a combination of local and systemic action of E. coli endotoxin is involved in the priming of milk PMN during mastitis. chemiluminescence / polymorphonuclear leukocyte / lipopolysaccharide / milk / blood Vet. Res. 32 (2001) [131][132][133][134][135][136][137][138][139][140][141][142][143][144] 131

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Section: Chemiluminescence Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…During E. coli mastitis, the ROI production of blood PMN has been observed to decrease [15]. The ROI production of milk PMN during mastitis, however, is not well characterized.…”

Section: Introductionmentioning

confidence: 99%

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Local and systemic effects of endotoxin mastitis on the chemiluminescence of milk and blood neutrophils in dairy cows

et al. 2001

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“…In infected mammary glands PMN are the predominant cell type. Earlier studies have shown that SCC prior to infection determines the sensitivity and severity of mastitis in lactating cows [4,21]. The protective effect of a high SCC against mastitis pathogens is well known [15].…”

Section: Introductionmentioning

confidence: 99%

Apoptosis and necrosis of blood and milk polymorphonuclear leukocytes in early and midlactating healthy cows

et al. 2001

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-Increased milk somatic cell counts (SCC) are used as an indicator for bovine mastitis. During mastitis, polymorphonuclear leukocytes (PMN) become the predominant cell type. Shortly after parturition, the severity of mastitis is increased and several PMN functions are downregulated. Apoptotic and necrotic processes of PMN could influence SCC and PMN functions. In this study, the percentages of apoptotic and necrotic PMN in blood and milk from early and midlactating healthy cows were compared. Apoptosis and necrosis of PMN were quantified using a dual-color flow cytometric procedure with fluorescein labeled annexin-V (green) and propidium iodide (red). Using this technique three different subpopulations of bovine PMN could be detected: apoptotic cells (high intensive green fluorescence), necrotic cells (high intensive green and high intensive red fluorescence) and viable cells (low intensive green and low intensive red fluorescence). Following a 4 h incubation of blood from both groups of cows at 37°C to induce apoptosis, the mean percentage of apoptotic blood PMN was significantly higher (P < 0.01) in early lactating cows (15.1%, n = 9) compared with midlactating cows (5.3%, n = 10). The mean percentage of necrotic PMN remained lower than 5% in all cows. In contrast to blood, no significant difference was found between the percentage of apoptotic PMN in milk from early (41.2%, n = 7) and midlactating cows (34.0%, n = 8). The percentage of necrotic PMN in milk from early lactating cows (25.9%, n = 7) was significantly higher than that in midlactating cows (14.2%, n = 8) (P < 0.05). Higher percentages of apoptotic as well as necrotic PMN were consistently found in milk compared to blood in all cows. From these results, it can be concluded that spontaneously induced apoptosis was higher in blood PMN from early lactating cows than in blood PMN from midlactating cows. The higher percentage of necrotic milk PMN in early lactating cows than in midlactating cows could be explained by the induction of secondary necrosis. bovine neutrophil / apoptosis / necrosis / blood / milk Vet. Res. 32 (2001) 617-622 617

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A comparative study of bovine blood and milk neutrophil functions with luminol‐dependent chemiluminescence

et al. 2001

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In this study, a technique was developed for the chemiluminescence (CL) measurement of bovine milk polymorphonuclear leukocytes (PMN). In the first study, the effects of cell number and the concentration of phorbol-12-myristate-13-acetate (PMA), luminol, latex bead particles, dimethyl sulphoxide (DMSO) and gelatin on the luminol-dependent cellular CL (LDCL) response were assessed with healthy cows in different stages of lactation. In the second study, the LDCL and in vitro bactericidal activity of blood and milk PMN towards Staphylococcus aureus was investigated. In general, the CL activity of blood PMN was consistently higher than that of milk PMN. We found that (a) the optimal cell density in blood and milk cells for maximal LDCL response ranged from 1.5 x 10(6) to 5 x 10(6) cells/mL; (b) the optimal concentrations of PMA, latex beads and luminol for maximal LDCL response were 100-200 ng/ml, 500 particles/PMN and 0.1 mmol/L, respectively. Concentrations of DMSO of 0.5-1% (v/v) did not significantly affect the maximal CL response of PMN. Gelatin concentrations of 0.1 -0.5 mg/ml had no effect on the LDCL of PMN. In addition, the LDCL of PMN was significantly correlated with bactericidal activity towards S. aureus (r = 0.78, p < 0.001 for blood PMN and r = 0.66, p < 0.01 for milk PMN). Under the optimal experimental conditions for measurement of CL produced by bovine blood and milk PMN defined in this study, LDCL assay is an accurate and reproducible technique for the rapid quantification of PMN bactericidal activity in physiological and pathological conditions of high-yielding dairy cows.

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Interaction Between the Respiratory Burst Activity of Neutrophil Leukocytes and Experimentally Induced Escherichia coli Mastitis in Cows

Cited by 106 publications

References 12 publications

Local and systemic effects of endotoxin mastitis on the chemiluminescence of milk and blood neutrophils in dairy cows

Local and systemic effects of endotoxin mastitis on the chemiluminescence of milk and blood neutrophils in dairy cows

Apoptosis and necrosis of blood and milk polymorphonuclear leukocytes in early and midlactating healthy cows

A comparative study of bovine blood and milk neutrophil functions with luminol‐dependent chemiluminescence

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