Interaction between the IgG-Fc fragment and subfragments and the IgG-Fc receptor on human platelets

Endresen, G. K. M.

doi:10.1016/0049-3848(89)90015-7

Cited by 3 publications

(1 citation statement)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These measurement demonstrated no differences in the kinetic parameters for intact IgG and its Fc (molecular weight ratio, «3:1). Although earlier binding assays produced (conflicting) results suggesting significant differences between the binding of intact IgG and Fc to cell-surface Fc receptors (Dissanayake & Hay, 1975;Unkeless & Eisen, 1975), more recent functional studies, including measurements of sideways killing (Ito et al, 1989), platelet aggregation (Endresen, 1989) , binding to virus-induced Fc receptors (Litwin et al, 1990) , and prostaglandin synthesis (Suzuki, 1991), have not shown significant differences.…”

Section: Effect Of Iggmentioning

confidence: 99%

Dissociation Kinetics between a Mouse Fc Receptor (Fc.gamma.RII) and IgG: Measurement by Total Internal Reflection with Fluorescence Photobleaching Recovery

Hsieh¹,

Thompson²

1995

Biochemistry

View full text Add to dashboard Cite

Total internal reflect with fluorescence photobleaching recovery (TIR-FPR) has been used to examine the dissociation kinetics between monomeric mouse IgG and a mouse Fc receptor (moFc gamma RII) reconstituted into substrate-supported planar membranes. IgG1, IgG2a, and IgG2b exhibited similar dissociation kinetics, whereas IgG3 did not bind. The fluorescence recovery curves for the IgG-moFc gamma RII interactions were best described by two reversible components (1.4 s-1, 66% and 0.06 s-1, 18%) and an irreversible component ( < 0.01 s-1, 16%). The kinetic parameters for a mouse anti-dinitrophenyl (DNP) IgG1 antibody were equivalent in the absence and presence of saturating amounts of DNP-glycine, demonstrating that possible allosteric changes which might occur in IgG1 upon hapten binding do not appreciably affect the kinetic characteristics of moFc gamma RII binding. The fluorescence recovery curves for polyclonal mouse IgG Fc were similar to those for intact IgG, showing that decreasing the size of the IgG 3-fold does not alter the dissociation rate. The dissociation kinetics of IgG1 decreased considerably in a low ionic strength buffer, indicating that the IgG1-moFc gamma RII interaction has significant electrostatic components.

show abstract

Section: Effect Of Iggmentioning

confidence: 99%

Dissociation Kinetics between a Mouse Fc Receptor (Fc.gamma.RII) and IgG: Measurement by Total Internal Reflection with Fluorescence Photobleaching Recovery

Hsieh¹,

Thompson²

1995

Biochemistry

View full text Add to dashboard Cite

show abstract

Differences in the platelet proaggregatory activity of immune complexes isolated from patients with myocardial infarction or pulmonary cancer

et al. 1999

View full text Add to dashboard Cite

Immune complexes were isolated immediately after the onset of the symptoms of myocardial infarction on an anti-Clq affinity column. The platelet proaggregatory effects of these immune complexes were compared with those isolated from patients suffering from pulmonary cancer. A markedly increased proaggregatory effect of immune complexes derived from patients with myocardial infarction and changes in the sensitivity of platelets from healthy volunteers to PGI(2) (prostacyclin) and PGD(2) were found. In contrast, immune complexes from patients with pulmonary cancer did not show any significant effect. The antigenic part of immune complexes is probably relevant in the induction of platelet aggregation.

show abstract

Differences in the platelet proaggregatory activity of immune complexes isolated from patients with myocardial infarction or pulmonary cancer

Blaskó¹,

Pal²,

Das³

et al. 1999

CPLA

View full text Add to dashboard Cite

show abstract

Interaction between the IgG-Fc fragment and subfragments and the IgG-Fc receptor on human platelets

Cited by 3 publications

References 17 publications

Dissociation Kinetics between a Mouse Fc Receptor (Fc.gamma.RII) and IgG: Measurement by Total Internal Reflection with Fluorescence Photobleaching Recovery

Dissociation Kinetics between a Mouse Fc Receptor (Fc.gamma.RII) and IgG: Measurement by Total Internal Reflection with Fluorescence Photobleaching Recovery

Differences in the platelet proaggregatory activity of immune complexes isolated from patients with myocardial infarction or pulmonary cancer

Differences in the platelet proaggregatory activity of immune complexes isolated from patients with myocardial infarction or pulmonary cancer

Contact Info

Product

Resources

About