2012
DOI: 10.3390/toxins4090729
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Interaction between Shiga Toxin and Monoclonal Antibodies: Binding Characteristics and in Vitro Neutralizing Abilities

Abstract: Monoclonal antibodies (MAbs) have been employed either for diagnosis or treatment of infections caused by different pathogens. Specifically for Shiga toxin-producing Escherichia coli (STEC), numerous immunoassays have been developed for STEC diagnosis, showing variability in sensitivity and specificity when evaluated by reference laboratories, and no therapy or vaccines are currently approved. Thus, the aim of this work was the characterization of the interaction between MAbs against Stx1 and Stx2 toxins and t… Show more

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Cited by 35 publications
(57 citation statements)
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“…Sensitivities and specificities vary according to the test format and the manufacturer 199, 200, 201, 202, 203, 204, 205. Nevertheless, the standard by which each manufacturer evaluates its tests also varies; therefore, a direct comparison of performance characteristics of various immunoassays has not been performed 198, 206, 207. Moreover, these commercially available tests are not affordable for developing countries.…”
Section: Enterohemorrhagic (Shiga Toxin-producing) E Coli (Ehec/stec)mentioning
confidence: 99%
See 1 more Smart Citation
“…Sensitivities and specificities vary according to the test format and the manufacturer 199, 200, 201, 202, 203, 204, 205. Nevertheless, the standard by which each manufacturer evaluates its tests also varies; therefore, a direct comparison of performance characteristics of various immunoassays has not been performed 198, 206, 207. Moreover, these commercially available tests are not affordable for developing countries.…”
Section: Enterohemorrhagic (Shiga Toxin-producing) E Coli (Ehec/stec)mentioning
confidence: 99%
“…Moreover, these commercially available tests are not affordable for developing countries. Thus, to outline this, previous works have established different formats of immunoassays, employing either a mixture of rabbit anti-Stx1 and anti-Stx2 sera by indirect ELISA or polyclonal and monoclonal antibodies in a capture ELISA assay for the detection of STEC 207, 208, 209. The standardized methods are reproducible, fast, easy to perform, showing high sensitivity in detecting Stx by capture ELISA, even in low-producing isolates.…”
Section: Enterohemorrhagic (Shiga Toxin-producing) E Coli (Ehec/stec)mentioning
confidence: 99%
“…Monoclonal antibodies (MAbs) have been shown to reduce the cytotoxicity of Stx1 and Stx2 in vitro (381), while Urtoxazumab, a humanized MAb against Stx2, was shown to be safe in a phase 1 study (382). Similarly, phase 1 clinical trials of chimeric monoclonal Stx1-and Stx2-neutralizing antibodies were also shown to be generally well tolerated in injected volunteers (383,384).…”
Section: Clinical Considerationsmentioning
confidence: 99%
“…2006) e para a toxina Stx de STEC (MENDES-LEDESMA et al, 2008;ROCHA et al, 2012 O sistema mais comumente utilizado para a expressão de proteínas heterólogas é o que utiliza E. coli como célula hospedeira (LEWIN, 1994(LEWIN, , 1997STRYER, 1988;ZAHA et al, 1996). Este sistema é amplamente difundido deviso à facilidade e baixo custo de se cultivar esta bactéria, e pela reprodutibilidade e abundância de proteínas que a mesma produz.…”
Section: Purificação Do Anticorpo Recombinante Psmt3 Scfv-intimina Pounclassified