Interaction between human neutrophils and corneocytes: Corneocyte-induced respiratory burst of neutrophils assessed by chemiluminescence

Abstract

In an in vitro study to examine whether corneocytes stimulate neutrophils to cause a respiratory burst, we found that stratum corneum (SC) homogenates obtained from the sole of healthy individuals induced a substantial respiratory burst in human neutrophils when assessed by lucigenin-dependent chemiluminescence (CL) in the presence of the fresh human serum. Electron microscopically, the interaction between corneocytes and neutrophils was shown as distinctive deformation of the neutrophils adhering to the surface of the corneocytes that suggested a specific binding between SC and neutrophils. In contrast, in the heat-inactivated serum-supplemented system, the peak intensity of SC-induced CL was significantly decreased, being only slightly higher than that noted in the SC-free background. To circumvent the time-consuming preparation of the SC homogenates, we measured CL with a sheet of SC obtained by stripping with adhesive cellophane tape. In this case we used plasma because the complement was easily activated by cellophane tape itself when serum was used. To evaluate the influence of the location of horny tissue in the SC on CL in neutrophils, we used SC sheets stripped with cellophane tape from various levels of the SC. However, there was no significant difference in CL responses between SC sheets obtained by 1, 2, 3, 5, 10, or 15 strippings. Our findings suggest that when SC comes in contact with serum, it is opsonized by C3b, and such SC causes a respiratory burst of neutrophils following their specific binding on the surface of corneocytes.

Role of neutrophils in induction of acute inflammation in T‐cell‐mediated immune dermatosis, psoriasis: A neutrophil‐associated inflammation‐boosting loop

“…Furthermore, the existence of PMNs and parakeratosis is also consistent with underlying epidermal hyperplasia, which shows a high mitotic rate (65). Coexistence of PMNs and parakeratosis is in part explained by the tissue-destructive properties of active oxygen intermediates and proteolytic enzymes that are released by activated PMNs attached to the stratum corneum (81,82) that are opsonized by iC3b through the alternative complement activation (83).…”

Point‐of‐care detection of neutrophils in live skin microsamples using chemiluminescence

“…Chemiluminescence is a well established procedure to measure activation of neutrophils as reported in the literature . In these reports, light emission is measured as a function of time in high concentrations of neutrophils (1E+6 cells) .…”

Influence of staphylococcal lipoteichoic acid on the frustrated phagocytosis of neutrophils against opsonized corneocytes

“…When normal SC comes in contact with serum, complement is activated mainly through the alternative pathway to generate cbemotactic peplide C5a anaphylatoxin (4). The attracted neutrophils specificially bind to the SC opsonized by C3b eotnponents in this process to induce a respitatory burst (5,6). Once the thin SC is broken to allow the invasion of tnicroorganistns, it is reasonable to presume that bacterial virulence factors such as protein A and lipoteichoic acid (LTA) modify the intetaction between the opsonized SC and neutrophils.…”