Essential oil (EO) production is the main objective of Damask rose cultivation but its stability at different environments has not been well documented. In order to this, 35 landraces of Damask rose were studied at 7 locations in Iran during 2007-8. The analysis of variance revealed significant differences (p≤0.01) in EO among landraces (G), locations (L), Years (Y/L) and for landrace×location (GL) interaction. A large portion of EO variation was attributed to environment (Location-year) and landrace×environment (GE) effects. The GL interaction was mainly crossover and mainly accounted for by non-linear (unpredictable) component. The total mean of EO was 286ppm and Kashan with 681 ppm and the landraces of SM2, SM1, AK1 and BA1 with 547, 442, 440 and 427 ppm respectively produced the highest oil among studied locations and landraces. According to the results, most of landraces that originated from warm temperate and arid regions produced higher EO than those from cool, semi-arid and humid regions. There was high consistency of stable genotypes with two stability models of Eberhart & Russell 1 and Shukla's 2 stability variance (σi 2) and according to them the landraces of CM1, QM1, KZ1, KS1 and HO1 were general stable with dynamic stability concept. In addition, we recommend the landrace of CM1 as moderate essential oil with general stability and adaptability in different environments and locations, furthermore, SM1 and BA1 can be recommended as superior essential oil with specific adaptability for warm and arid of the southern part (Khuzestan, etc.) and SM2 and AK1 for temperate and cool areas of the northern half (Isfahan, Khorasan etc.) in Iran.