Interaction between antifreeze protein and ice crystal facet evaluated by ice-channel electrophoretic measurements of threshold electric field strength

Inagawa, Arinori; Uehara, Nobuo; Okada, Tetsuo

doi:10.1016/j.aca.2020.03.025

Cited by 6 publications

(5 citation statements)

References 47 publications

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“…AFPs can prevent the growth of ice crystals and can decrease the freezing point of the solution. Because of their THA, AFPs are the most important factor in the antifreeze process of cells and organisms (Inagawa et al, 2020; Tsuda et al, 2020; Ye et al, 2020). We identified one AFP with a THA of 0.97°C in a previous study (Yu et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analyses and weighted gene coexpression network analysis reveal key pathways and genes involved in the rapid cold resistance of the Chinese white wax scale insect

Zhang

Liu

et al. 2021

Arch Insect Biochem Physiol

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The Chinese white wax scale insect, Ericerus pela, is an important resource insect in China. The rapid response of E. pela to decreasing temperatures plays key roles in the population distribution. In this study, we analyzed the gene expression of E. pela treated with low temperature using transcriptome analyses and weighted gene coexpression network analysis (WGCNA). The results showed that the cold resistance of E. pela involved changes in the expression of many genes. The genes were mainly involved in alcohol formation activity, lipid metabolism, membrane and structure, and oxidoreductase activity. According to the WGCNA results, some pathways related to cold resistance were found in the genes in the modules, such as cytoskeleton proteins, cytoskeleton protein pathway, biosynthesis of unsaturated fatty acids, glycerophospholipid metabolism, ether lipid metabolism, and thermogenesis. Some of the hub genes were nonspecific lipid‐transfer proteins, DnaJ homolog subfamily C member 13, paramyosin, tropomodulin, and tubulin beta chain. In particular, the hub genes of the tan module included the heat shock protein (hsp) 10, hsp 60, hsp 70, and hsp 90 genes. Thirty‐five antifreeze protein (afp) genes were identified according to the annotation results. Three afp genes were further identified among the hub genes. Six of these genes were selected for heterogeneous protein expression. One of them was expressed successfully. The thermal hysteresis activity (THA) analyses showed that the THA was 1.73°C. These results showed that the cytoskeleton, lipid metabolism, thermogenesis, HSPs and AFPs may play important roles in the cold resistance of E. pela.

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Section: Discussionmentioning

confidence: 99%

Transcriptome analyses and weighted gene coexpression network analysis reveal key pathways and genes involved in the rapid cold resistance of the Chinese white wax scale insect

Zhang

Liu

et al. 2021

Arch Insect Biochem Physiol

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“…We have shown in our previous studies that the FCS channel can be used to evaluate chemical phenomena. ,, Because the FCS exists in natural ice, it can be considered a unique chemical field for biological and atmospheric sciences. Some chemical reactions can be evaluated based on the surface reactions of the microspheres and their aggregation.…”

Section: Discussionmentioning

confidence: 99%

“…The through-channel of the FCS in frozen aqueous sucrose was introduced into a capillary as a septum for separating micro- and nanoparticles . The size tunability of the FCS was used to control the chemical interaction between prism planes of ice and antifreeze proteins for evaluating the interaction force. , Although we have shown the potential of the FCS as a separation field, only size-selective recognition, which is the observation of the migration behavior, was proven. This is because the direction of the FCS was disordered, preventing the migrating target from being collected at the desired outlet.…”

Section: Introductionmentioning

confidence: 99%

“…In our previous study, we experimentally discovered that seeding the ice crystals to the aqueous sucrose solutions at higher freezing temperature can grow large ice crystals, forming ca. 1 mm long FCS channels by the Mullins-Sekerka instability. , In these studies, the volume of the freezing matrix is relatively small. This leads to the efficient heat conduction and homogeneous temperature distribution in the sample.…”

Section: Introductionmentioning

confidence: 99%

“…1 mm long FCS channels by the Mullins-Sekerka instability. 39,46 In these studies, the volume of the freezing matrix is relatively small. This leads to the efficient heat conduction and homogeneous temperature distribution in the sample.…”

Section: ■ Introductionmentioning

confidence: 99%

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2023

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Self Cite

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A frozen aqueous solution was integrated into a microfluidic device as a size-tunable separation field for the size-selective separation of micro/ nanospheres. The width of the ice grain boundaries formed in frozen aqueous solutions could be altered by controlling the operating temperature. A freezing chamber was placed adjacent to the microfluidic channel. A sample-dispersing aqueous sucrose solution was injected into the chamber and frozen, allowing the freeze-concentrated solution (FCS) to run vertically to the microfluidic channel, where the eluting solution flows. The operating temperature can be used to control the physical interaction between the ice wall and micro/nanospheres, enabling sizeselective migration. The eluted micro/nanospheres in the microchannel were passed through the eluting solution collected from the outlet. We achieved size-selective separation and collection of microspheres and nanospheres. We separated the exosomes and yeast cells to demonstrate their applicability in bioseparation. The present method is suitable not only for size-selective separation but also for evaluating the biological expression of extracellular vesicles under cryogenic conditions.

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Interaction between antifreeze protein and ice crystal facet evaluated by ice-channel electrophoretic measurements of threshold electric field strength

Cited by 6 publications

References 47 publications

Transcriptome analyses and weighted gene coexpression network analysis reveal key pathways and genes involved in the rapid cold resistance of the Chinese white wax scale insect

Transcriptome analyses and weighted gene coexpression network analysis reveal key pathways and genes involved in the rapid cold resistance of the Chinese white wax scale insect

Incorporation of a Morphologically Controlled Ice Grain Boundary into a Microfluidic Device for Size-Selective Separation of Micro/Nanospheres

Absorption-based colorimetric detection of nickel(II) ion by phase separation of thermoresponsive magnetic nanoparticles under microflow

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