Transcriptome analyses and weighted gene coexpression network analysis reveal key pathways and genes involved in the rapid cold resistance of the Chinese white wax scale insect

Zhang, Hongping; Liu, Wei; An, Jia-Qi; Yang, Pu; Guo, Lihong; Li, Yanqiong; Lv, Jing; Yu, Shaohua

doi:10.1002/arch.21781

Cited by 12 publications

(11 citation statements)

References 38 publications

(55 reference statements)

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“…Eight genes coding UDP-glucuronosyltransferases were determined to be the hub genes in insecticide resistance [ 80 ], which proved essential for the elimination and detoxification of drugs, xenobiotics, and chemical compounds [ 81 , 82 ]. In the study of Ericerus pela , the hub genes associated with cold resistance were identified by using WGCNA; some well-studied cold resistance-related genes, such as heat shock protein, and antifreeze protein, could be rediscovered by this method [ 83 ]. Through these studies, it was confirmed that many of the hub genes obtained by WGCNA analysis were indeed very important genes; the WGCNA method is reliable.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analyses Provide Insights into the Aggressive Behavior toward Conspecific and Heterospecific in Thitarodes xiaojinensis (Lepidoptera: Hepialidae)

Rao

Cao

et al. 2021

Insects

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Aggressive behavior in animals is important for survival and reproduction. It is well studied in adult insects, such as flies, ants, honey bees, and crickets. However, the larvae of Lepidopteran insects are also aggressive, studies of which are still lacking. Here, RNA-seq was used to generate a high-quality database for the aggressive behavior of Thitarodes xiaojinensis toward conspecifics and heterospecifics. Although there was similar aggressive behavior between the conspecific group and heterospecific group, significant differences were identified at the transcriptional level. When there was aggressive behavior toward conspecifics, T. xiaojinensis trended toward higher expression at the respiratory chain, while cuticle development and metabolism may have interfered. On the other hand, when there was aggressive behavior toward H. armigera, genes related to neuron and cuticle development, cellular processes, and its regulated signaling pathways were significantly upregulated, while the genes associated with oxidation-reduction and metabolism were downregulated. Weighted gene co-expression networks analysis (WGCNA) was performed, and two modules with properties correlating to the aggressive behavior of T. xiaojinensis were identified. Several hub genes were predicted and confirmed by qRT-PCR, such as CLTC, MYH, IGF2BP1, and EMC. This study provides a global view and potential key genes for the aggressive behavior of T. xiaojinensis toward conspecifics and heterospecifics. Further investigation of the hub genes would help us to better understand the aggressive behavior of insects.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analyses Provide Insights into the Aggressive Behavior toward Conspecific and Heterospecific in Thitarodes xiaojinensis (Lepidoptera: Hepialidae)

Rao

Cao

et al. 2021

Insects

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“…Most Coccoidea species are agriculture and forestry pests, except for very few species with a high economic value, such as the Chinese white wax scale insect Ericerus pela (Chavannes, 1848) [ 17 ]. Previous work has related to E. pela ’s use in biological research, molecular research, and the popularization of relevant applications [ 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ]. Regarding its breeding history, people initially started to collect white wax as early as approximately the 3rd century AD in China [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…Previous work has related to E. pela ’s use in biological research, molecular research, and the popularization of relevant applications [ 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ]. Regarding its breeding history, people initially started to collect white wax as early as approximately the 3rd century AD in China [ 26 ]. The wax produced by insects is an important commodity that has been widely applied in cosmetics, Chinese traditional medicine, the manufacturing industry (e.g., automotive and furniture manufacture), and the printing industry for more than one thousand years [ 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

The Complete Mitochondrial Genome of the Chinese White Wax Scale Insect, Ericerus pela Chavannes (Hemiptera: Coccidae), with Novel Gene Arrangement and Truncated tRNA Genes

Wei

et al. 2023

Insects

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The Chinese white wax scale insect, Ericerus pela Chavannes (Hemiptera: Coccidae), is one of the scale insects with great economic value and has been dispersed and reared in China for over one thousand years. Its mitochondrial genome provides essential information for the molecular identification and genetic study of this species. We assembled the complete mitochondrial genome of E. pela based on PacBio sequencing and analyzed its genomic features. The genome was 17,766 bp in length with 13 protein-coding genes, 22 tRNAs, and two rRNA genes. The analysis results showed E. pela had significant gene rearrangements involving tRNAs compared with other Coccoidea species. Furthermore, E. pela’s nine tRNAs were identified to have obvious truncated structures. The phylogenetic tree compiled of the species showed a long branch of the Coccoidea lineage, which indicated the high evolutionary rate in this group. Our study revealed the mitochondrial characteristics of E. pela and enriched the mitochondrial genetic information on Coccoidea species. It also determined the occurrence of gene rearrangement for the species in this superfamily.

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“…, 2021; Zhang et al. , 2021a), insecticide resistance (Tian et al. , 2021), aggressive behavior (Rao et al.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the signaling pathways of wing dimorphism regulated by biotic and abiotic stress in the brown planthopper

Chen

et al. 2022

Insect Science

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Wing polymorphism is an evolutionary trait that is widely present in various insects and provides a model system for studying the evolutionary significance of insect dispersal. The brown planthopper (BPH, Nilaparvata lugens) can alter its wing morphs under biotic and abiotic stress. However, whether differential signaling pathways are induced by the 2 types of stress remain largely unknown. Here, we screened a number of candidate genes through weighted gene co-expression network analysis (WGCNA) and found that ornithine decarboxylase (NlODC), a key enzyme in the synthesis of polyamines, was associated with wing differentiation in BPH and mainly responded to abiotic stress stimuli. We analyzed the Kyoto Encyclopedia of Genes and Genomes enrichment pathways of differentially expressed genes under the 2 stresses by transcriptomic comparison, and found that biotic stress mainly influenced insulin-related signaling pathways while abiotic stress mainly influenced hormone-related pathways. Moreover, we found that insulin receptor 1 (NlInR1) may regulate wing differentiation of BPH by responding to both biotic and abiotic stress, but NlInR2 only responded to biotic stress. Similarly, the juvenile hormone epoxide hydrolase associated with juvenile hormone degradation and NlODC may regulate wing differentiation mainly through abiotic stress. A model based on the genes and stresses to modulate the wing dimorphism of BPH was proposed. These findings present a comprehensive molecular mechanism for wing polymorphism in BPH induced by biotic and abiotic stress.

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Transcriptome analyses and weighted gene coexpression network analysis reveal key pathways and genes involved in the rapid cold resistance of the Chinese white wax scale insect

Cited by 12 publications

References 38 publications

Transcriptome Analyses Provide Insights into the Aggressive Behavior toward Conspecific and Heterospecific in Thitarodes xiaojinensis (Lepidoptera: Hepialidae)

Transcriptome Analyses Provide Insights into the Aggressive Behavior toward Conspecific and Heterospecific in Thitarodes xiaojinensis (Lepidoptera: Hepialidae)

The Complete Mitochondrial Genome of the Chinese White Wax Scale Insect, Ericerus pela Chavannes (Hemiptera: Coccidae), with Novel Gene Arrangement and Truncated tRNA Genes

Comparison of the signaling pathways of wing dimorphism regulated by biotic and abiotic stress in the brown planthopper

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