Interacción de rotavirus con la proteína disulfuro-isomerasa in vitro y en sistemas celulares

Calderón, Martha Nancy; Guerrero, C.; Domínguez, Yohana; Garzón, Eliana; Barreto, Sandra M; Acosta, Orlando

doi:10.7705/biomedica.v31i1.337

Cited by 4 publications

(6 citation statements)

References 57 publications

(69 reference statements)

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“…However, it is important to clarify that Hsc70 and β3-integrin are not the only receptors for RV. Other molecules are also involved in this RV infection [11,16,25,26]. In this study, it was also shown that probiotic extracts can bind to MA104 cells, possibly via electrostatic interactions between cellular receptors and biding motifs in the extract [27,28].…”

Section: Discussionmentioning

confidence: 61%

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Decreased rotavirus infection of MA104 cells via probiotic extract binding to Hsc70 and ß3 integrin receptors

et al. 2018

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Probiotic bacteria are microorganisms beneficial to human health, useful to improving biological conditions. Thanks to probiotic bacteria the symptoms of viral infections can be alleviated. Different mechanisms whereby probiotic bacteria exert they antiviral effect have been proposed. The aim of this study was to determine whether probiotic bacteria extracts bind to receptors of host cells susceptible of rotavirus (RV) infection. To accomplish this objective, four probiotic bacterial strains of Lactobacillus spp. and Bifidobacterium spp. were tested. Probiotic extracts were obtained after bacterial growth, cell lysis and centrifugation. Obtained probiotic extracts were used in assays to interfere with adhesion and penetration of a RV strain in the mammal cell line MA104. Furthermore, the interaction between probiotic extracts and MA104 cell receptors was evaluated by co-immunoprecipitation assays using anti-ß3-integrins and anti-Hsc70 antibodies. All four probiotic, protein-rich, extracts reduced RV infections in MA104 cells, suggesting a successful antiviral activity mediated by these probiotic extracts. All probiotic extracts significantly exerted thir antiviral activity by interfering with RV adhesion on MA104 cell receptors, with proteins in probiotic extracts competitively interacting with cell surface receptors necessary to RV infection. Co-immunoprecipitation assay results showed that proteins in probiotic extracts were able to bind to ß3-integrinsand Hsc70, which are two cellular receptors required to viral infection. The most significant contribution of this study is an insight into the mechanisms of probiotic antiviral activity, thus expanding current probiotics fundamental knowledge.

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Section: Discussionmentioning

confidence: 61%

“…The mechanism of cell infection of several RV strains has been hypothesized to be mediated by sialic acid residues on the cell membrane, acting as receptors. However, RV infection also requires co-receptors, such as integrins (i.e., α2β1, αvβ3, αxβ2, and α4β1) and thermal shock proteins (i.e., hsc70) [10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Decreased rotavirus infection of MA104 cells via probiotic extract binding to Hsc70 and ß3 integrin receptors

et al. 2018

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“…Here we have shown that the cellular proteins reacting with their respective antibodies fall within the three main types of cell surface proteins required in the entry into the host cell 36 . For instance, integrin β3 is a cell surface molecule that mediates rotavirus attachment to cell 35 , 56 while rotavirus entry needs chaperone activities and redox reactions performed by HSPs/Hsc70 33 , 61 , 62 and PDI 37 , 57 , respectively. In addition, specific inhibition of Hsp90 has been reported to decrease infection by human rotavirus strain KU and simian rotavirus strain SA11 through modulation of cellular signaling proteins 61 .…”

Section: Resultsmentioning

confidence: 99%

“…In this regard, we wanted to study the implication of some cell surface proteins in the infection of Sp2/0-Ag14 cells by rotavirus isolates and the changes in the expression of these cellular proteins induced by the viral infection. Cell surface Hsc70, PDI, and integrin αVβ3 have been shown to interact with rotavirus structural proteins during entry into MA104 or Caco-2 cells (40,56,57). These cellular proteins, in addition to some HSP, have also been associated with cell malignancy (58,59).…”

Section: Discussionmentioning

confidence: 99%

Assessing the oncolytic potential of rotavirus on mouse myeloma cell line Sp2/0-Ag14

Guerrero

Guzmán

et al. 2020

biomedica

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Introduction: Cancer is the second leading cause of death in the United States, surpassed only by cardiovascular disease. However, cancer has now overtaken cardiovascular disease as the main cause of death in 12 countries in Western Europe. The burden of cancer is posing a major challenge to health care systems worldwide and demanding improvements in methods for cancer prevention, diagnosis, and treatment. Alternative and complementary strategies for orthodox surgery, radiotherapy, and chemotherapy need to be developed.Objective: To determine the oncolytic potential of tumor cell-adapted rotavirus in terms of their ability to infect and lysate murine myeloma Sp2/0-Ag14 cells.Materials and methods: We inoculated rotaviruses Wt1-5, WWM, TRUYO, ECwt-O, and WTEW in Sp2/0-Ag14 cells and we examined their infectious effects by immunocytochemistry, immunofluorescence, flow cytometry, and DNA fragmentation assays.Results: Rotavirus infection involved the participation of some heat shock proteins, of protein disulfide isomerase (PDI), and integrin β3. We detected the accumulation of viral antigens within the virus-inoculated cells and in the culture medium in all the rotavirus isolates examined. The rotavirus-induced cell death mechanism in Sp2/0-Ag14 cells involved changes in cell membrane permeability, chromatin condensation, and DNA fragmentation, which were compatible with cytotoxicity and apoptosis.Conclusions: The ability of the rotavirus isolates Wt1-5, WWM, TRUYO, ECwt-O, and WTEW to infect and cause cell death of Sp2/0-Ag14 cells through mechanisms that are compatible with virus-induced apoptosis makes them potential candidates as oncolytic agents.

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“…The entry of viruses into target cells shows a specific cell tropism involving the presence of receptor molecules on the cell surface. Entry of neuraminidase-sensitive rotaviruses into MA104 cells seems to be a multistep event that incorporates attachment to sialylated glycan followed by interactions with other cell surface molecules such as heat shock cognate protein 70 (Hsc70) [50][51][52], protein disulfide isomerase (PDI) [53][54][55] and integrins αVβ3 [56,57], α2β1 [58,59] and αIVβ1 [60,61]. Although rotavirus belongs to the same family of reovirus, both viruses show significant differences in cell tropism, receptors used for entry, particle structure, genomic segments, replication mechanisms, and clinical manifestations.…”

Section: Introductionmentioning

confidence: 99%

Induction of Cell Death in the Human Acute Lymphoblastic Leukemia Cell Line Reh by Infection with Rotavirus Isolate Wt1-5

Guerrero

Acosta

2020

Biomedicines

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Cancer is a major health problem that poses a great challenge to health care systems worldwide. Tools for cancer treatment have rapidly advanced in recent years, resulting in therapeutic strategies which are alternative and complementary to conventional treatment. To identify the cell surface receptors used by a tumor cell-adapted rotavirus and the cell death markers induced by its infection, we use Wt1-5, a rotavirus isolate recently adapted to tumor cells, to infect the human acute lymphoblastic leukemia cell line, Reh. The expression of cell surface receptors used by Wt1-5 was determined using flow cytometry and an antibody blocking assay to test for their implication in virus infection. Viral antigens and cell death markers induced by rotavirus infection were followed by flow cytometric analysis. The present study showed that rotavirus Wt1-5 was able to use cell surface proteins such as heat shock proteins (HSPs) 90, 70, 60 and 40, Hsc70, PDI and integrin β3. Rotavirus Wt1-5 induced cytotoxic effects including changes in cell membrane permeability, alteration of mitochondrial membrane potential, DNA fragmentation and activation of cell death signaling. Wt1-5 deserves to be further studied as a candidate oncolytic agent due to its ability to induce apoptosis in lymphoblastic leukemia-derived cells.

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Interacción de rotavirus con la proteína disulfuro-isomerasa in vitro y en sistemas celulares

Cited by 4 publications

References 57 publications

Decreased rotavirus infection of MA104 cells via probiotic extract binding to Hsc70 and ß3 integrin receptors

Decreased rotavirus infection of MA104 cells via probiotic extract binding to Hsc70 and ß3 integrin receptors

Assessing the oncolytic potential of rotavirus on mouse myeloma cell line Sp2/0-Ag14

Induction of Cell Death in the Human Acute Lymphoblastic Leukemia Cell Line Reh by Infection with Rotavirus Isolate Wt1-5

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