Decreased rotavirus infection of MA104 cells via probiotic extract binding to Hsc70 and ß3 integrin receptors

Abstract

Probiotic bacteria are microorganisms beneficial to human health, useful to improving biological conditions. Thanks to probiotic bacteria the symptoms of viral infections can be alleviated. Different mechanisms whereby probiotic bacteria exert they antiviral effect have been proposed. The aim of this study was to determine whether probiotic bacteria extracts bind to receptors of host cells susceptible of rotavirus (RV) infection. To accomplish this objective, four probiotic bacterial strains of Lactobacillus spp. and Bifidobacterium spp. were tested. Probiotic extracts were obtained after bacterial growth, cell lysis and centrifugation. Obtained probiotic extracts were used in assays to interfere with adhesion and penetration of a RV strain in the mammal cell line MA104. Furthermore, the interaction between probiotic extracts and MA104 cell receptors was evaluated by co-immunoprecipitation assays using anti-ß3-integrins and anti-Hsc70 antibodies. All four probiotic, protein-rich, extracts reduced RV infections in MA104 cells, suggesting a successful antiviral activity mediated by these probiotic extracts. All probiotic extracts significantly exerted thir antiviral activity by interfering with RV adhesion on MA104 cell receptors, with proteins in probiotic extracts competitively interacting with cell surface receptors necessary to RV infection. Co-immunoprecipitation assay results showed that proteins in probiotic extracts were able to bind to ß3-integrinsand Hsc70, which are two cellular receptors required to viral infection. The most significant contribution of this study is an insight into the mechanisms of probiotic antiviral activity, thus expanding current probiotics fundamental knowledge.

Strain-Specific Probiotic Properties of Bifidobacteria and Lactobacilli for the Prevention of Diarrhea Caused by Rotavirus in a Preclinical Model

“…On the other hand, herein the reduction of viral shedding exerted by Lactobacillus is in line with other studies, which found that L. rhamnosus GG was able to reduce RV elimination in gnotobiotic piglets infected with human RV [14] and in children [59]. In vitro studies have reported that Bifidobacteria and Lactobacillus are able to inhibit RV infection [2,37], for example by interfering in the adhesion step. In this regard, it may be possible that glycans or other molecules found on the surface of the bacteria interact with the virus, therefore blocking the infection, similar to oligosaccharides present in breastmilk [70,71] and infant formula [13].…”

“…Probiotics are defined as "live microorganisms that, when administered in adequate amounts, confer a health benefit on the host" [1]. Several commensal microorganisms with probiotic properties are known to reside in the gastrointestinal tract of humans as part of the microbiota [2]. In this context, probiotics are currently being used to prevent illness, improve health, and treat intestinal diseases such as infections triggered by numerous pathogens, including viruses [2][3][4].…”

“…Several commensal microorganisms with probiotic properties are known to reside in the gastrointestinal tract of humans as part of the microbiota [2]. In this context, probiotics are currently being used to prevent illness, improve health, and treat intestinal diseases such as infections triggered by numerous pathogens, including viruses [2][3][4]. In the past decades there has been an increasing interest in the use of probiotics to prevent and ameliorate viral diarrhea [5,6].…”

The Anti-Rotaviral Activity of Low Molecular Weight and Non-Proteinaceous Substance from Bifidobacterium longum BORI Cell Extract

“…reported that protein rich extracts of Bifidobacterium spp. interfered with rotavirus adhesion on MA104 cells by binding b3-integrins and Hsc70 [17]. In addition, Chenoll et al found that proteinaceous substance, an 11-amino acid peptide, from supernatant of B. longum CECT 7210 inhibit the replication of rotavirus [18].…”

Treading a HOSTile path: Mapping the dynamic landscape of host cell–rotavirus interactions to explore novel host-directed curative dimensions

“…and Bifidobacterium spp. with integrin (β 3 ) binding capability blocked RV infectivity at the post-attachment step ( Figure 2 ) [ 61 , 65 , 66 ]. Moreover, the strain-specific anti-RV effects of the flavonoid genistein (but not of its inactive analogue daidzein) at the virus-host cell surface attachment stage has been explained to possibly stem from inhibition of integrin activation though the protein tyrosine kinase inhibition activity of genistein [ 67 ].…”