Inter simple sequence repeat markers to assess genetic diversity of the desert date (Balanites aegyptiaca Del.) for Sahelian ecosystem restoration

Abdelaziz, Selouka Mint; Medraoui, Leila; Alami, M.; Pakhrou, Ouafae; Makkaoui, Meryem; Boukhary, Ali Ould Mohamed Salem; Filali‐Maltouf, Abdelkarim

doi:10.1038/s41598-020-71835-9

Cited by 24 publications

(12 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ISSR primers were designed by Kalendar et al [24], and in this study ISSR primers for P. sylvestris were used [25]. DNA amplification was conducted using a slightly modified protocol [25,26]. A total of 20 primers were tested initially, and five polymorphic primers producing clearly identifiable and repeatable bands were selected for further analyses (Table 1).…”

Section: Methodsmentioning

confidence: 99%

Genetic Structure, Differentiation and Originality of Pinus sylvestris L. Populations in the East of the East European Plain

Vasilyeva

Chertov

Nechaeva

et al. 2021

Forests

View full text Add to dashboard Cite

In order to carry out activities aimed at conservation and rational use of forest resources; it is necessary to study the main forest-forming plant species in detail. Scots pine (Pinus sylvestris L., Pinaceae) is mainly found in the boreal forests of Eurasia and is not so often encountered in the east of the East European Plain. The aim of the study was to study the genetic diversity, structure and differentiation of Scots pine populations in the east of the East European Plain. We studied ten populations of P. sylvestris using the Inter Simple Sequence Repeats (ISSR)-based DNA polymorphism detection method. Natural populations are demonstrated by relatively high rates of genetic diversity (He = 0.167; ne = 1.279; I = 0.253). At the same time, there is a tendency for a decrease in the genetic diversity of the studied populations of P. sylvestris from west to east. Analysis of the genetic structure shows that the studied populations are highly differentiated (GST = 0.439), the intrapopulation component accounts for about 56% of the genetic diversity. Using various algorithms for determining the spatial genetic structure, it is found that the studied populations form two groups of populations in accordance with geographic location. With the help of a genetic originality coefficient, populations with specific and typical gene pools are identified. They are recommended as sources of genetic diversity and reserves for the conservation of genetic resources of the species.

show abstract

Section: Methodsmentioning

confidence: 99%

Genetic Structure, Differentiation and Originality of Pinus sylvestris L. Populations in the East of the East European Plain

Vasilyeva

Chertov

Nechaeva

et al. 2021

Forests

View full text Add to dashboard Cite

show abstract

“…B. aegyptiaca has been the subject of various classic studies because of its importance as a source of food and medicines. In traditional medicine, the fruit of this plant is used to treat diabetes, asthma, epilepsy, and malaria [ 5 ]. The seeds of B. aegyptiaca fruit have a high concentration of oils (46.0–54.7%), especially unsaturated fatty acids (up to 75% of total fatty acids), and proteins (26.1–34.3%).…”

Section: Introductionmentioning

confidence: 99%

Complete chloroplast genome of the desert date (Balanites aegyptiaca (L.) Del. comparative analysis, and phylogenetic relationships among the members of Zygophyllaceae

et al. 2022

View full text Add to dashboard Cite

Background Balanites aegyptiaca (L.) Delile, commonly known as desert date, is a thorny evergreen tree belonging to the family Zygophyllaceae and subfamily Tribuloideae that is widespread in arid and semiarid regions. This plant is an important source of food and medicines and plays an important role in conservation strategies for restoring degraded desert ecosystems. Results In the present study, we sequenced the complete plastome of B. aegyptiaca. The chloroplast genome was 155,800 bp, with a typical four-region structure: a large single copy (LSC) region of 86,562 bp, a small single copy (SSC) region of 18,102 bp, and inverted repeat regions (IRa and IRb) of 25,568 bp each. The GC content was 35.5%. The chloroplast genome of B. aegyptiaca contains 107 genes, 75 of which coding proteins, 28 coding tRNA, and 4 coding rRNA. We did not observe a large loss in plastid genes or a reduction in the genome size in B. aegyptiaca, as found previously in some species belonging to the family Zygophyllaceae. However, we noticed a divergence in the location of certain genes at the IR-LSC and IR-SSC boundaries and loss of ndh genes relative to other species. Furthermore, the phylogenetic tree constructed from the complete chloroplast genome data broadly supported the taxonomic classification of B. aegyptiaca as belonging to the Zygophyllaceae family. The plastome of B. aegyptiaca was found to be rich in single sequence repeats (SSRs), with a total of 240 SSRs. Conclusions The genomic data available from this study could be useful for developing molecular markers to evaluate population structure, investigate genetic variation, and improve production programs for B. aegyptiaca. Furthermore, the current data will support future investigation of the evolution of the family Zygophyllaceae.

show abstract

“…Analysis of molecular variance and population differentiation (FST) were conducted to determine whether the populations were genetically different from each other (GenAlEx 6.5b2). The dissimilarity matrix using the Jaccard index was generated using binary data [33]. The microsatellite data was converted into binary using GenAlEx 6.51b2.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Spider Plant (Cleome gynandra) Accessions Using SSR Markers

et al. 2021

View full text Add to dashboard Cite

Spider plant (Cleome gynandra L.) is an indigenous leafy vegetable that is a rich source of minerals, proteins, vitamins, flavonoids, antioxidants and many other compounds. Morphological characterization of the germplasm for optimal genetic exploitation is limited by environmental factors. The objective of this study was to determine the degree of genetic diversity between exotic and local spider plant accessions using molecular markers. The spider plant germplasm consisted of 18 local and exotic accessions. Seven polymorphic simple sequence repeat (SSR) markers were used in the study. The SSR marker combinations revealed a total of 46 alleles at 7 loci, with an average of 6.57 alleles per locus. An average polymorphic information index of 0.69 was recorded. According to Nei’s genetic distance analysis, low population differentiation was observed between the local and exotic spider plant accessions, which was indicated by a low fixation index value of 0.024 and a high Nm (10.20). Analysis of molecular variance showed no significant (p < 0.327) variation among and within the local and exotic accessions. Furthermore, low population differentiation was recorded when accessions were grouped based on their stem colour, with an Fst value of 0.007. In both the hierarchical clustering using an unweighted pair group method with arithmetic mean (UPGMA) and principal coordinate analysis (PCoA), the local accession ‘ML-3-KK’ was grouped alone, suggesting that this accession was different from the rest of the accessions and could be useful in selection programmes for spider plant genetic improvement. Both the UPGMA and PCoA were similar in clustering the eighteen spider plant accessions into four groups. The local spider plant accessions were more genetically diverse (with sixteen rare alleles) when compared to the exotic accessions (with only three rare alleles). The rare alleles provided useful information on the genetic variability in certain loci and identification of accessions that could be used as parental lines in spider plant breeding programmes.

show abstract

Inter simple sequence repeat markers to assess genetic diversity of the desert date (Balanites aegyptiaca Del.) for Sahelian ecosystem restoration

Cited by 24 publications

References 30 publications

Genetic Structure, Differentiation and Originality of Pinus sylvestris L. Populations in the East of the East European Plain

Genetic Structure, Differentiation and Originality of Pinus sylvestris L. Populations in the East of the East European Plain

Complete chloroplast genome of the desert date (Balanites aegyptiaca (L.) Del. comparative analysis, and phylogenetic relationships among the members of Zygophyllaceae

Molecular Characterization of Spider Plant (Cleome gynandra) Accessions Using SSR Markers

Contact Info

Product

Resources

About