Inter-Laboratory Study to Validate New Rapid Screening Methods for <i><i>Kudoa septempunctata</i></i>

Abstract

Kudoa septempunctata is the causative agent of a food-borne disease associated with the ingestion of raw olive flounder. As the current qRT-PCR method for its detection is timeconsuming, a rapid and simple method is required. Recently, a new real-time loop-mediated isothermal amplification LAMP method and an immunochromatography method, whose sensitivities are intended to be compatible with that designated in the official analytical method 10 5 spores/g olive flounder , have been developed. To validate these new methods, we performed an inter-laboratory study across seven laboratories. Both methods could not detect less than 10 4 spores/g; however, these methods were able to detect more than 10 5 spores/g in olive flounder samples. These results demonstrated that the sensitivities of these methods were compatible with the designated level in the official analytical method. We concluded that these new methods were acceptable as the screening methods for K. septempunctata.

Achieving sustainable and climate‐resilient fisheries requires marine ecosystem forecasts to include fish condition

“…Development of field‐based molecular tools, in the form of rapid diagnostic tests, that offer the same specificity and sensitivity as laboratory‐based diagnostics, would be of use in this regard (Ohnishi et al., 2016). Lateral‐flow immunochromatography, in particular, holds promise for more rapid screening of fish fillets at seafood processing factories.…”

“…Lateral‐flow immunochromatography, in particular, holds promise for more rapid screening of fish fillets at seafood processing factories. Innovations such as real‐time loop‐mediated isothermal amplification methods and immunochromatography can vastly improve the efficiency of collecting biological data on parasitic infection (Ohnishi et al., 2016) and harmful marine biotoxins (McPartlin et al., 2016) in the factory, and results of these tests can be then integrated into marine ecosystem forecasts.…”

In vitroeffect of two commercial anti-coccidial drugs against myxospores ofKudoa septempunctatagenotype ST3 (Myxozoa, Multivalvulida)

“…K. septempunctata has recently been linked to human food poisoning cases in Japan [15]. K. septempunctata spores have been isolated from raw olive flounder within 20 h after the fish was consumed by patients who developed diarrhoea, and the patients recovered completely within 24 h, suggesting that K. septempunctata spores are associated with transient clinical symptoms [10].…”

“…Several studies have reported clinical symptoms in animal models including the mouse [1, 7, 10], and permeability increase in the human intestinal epithelial monolayer [14]. Few reports on detection methods for K. septempunctata are available [10, 15, 16]. Contaminated fish occasionally reach the consumer through raw olive flounder fillets as infection is not tested for prior to consumption.…”