Inter-individual and intragenomic variations in the ITS region of Clonorchis sinensis (Trematoda: Opisthorchiidae) from Russia and Vietnam

Tatonova, Yulia V.; Челомина, Г. Н.; Nguyen, Hung Manh

doi:10.1016/j.meegid.2017.10.008

Cited by 15 publications

(9 citation statements)

References 52 publications

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“…Clonorchis sinensis: Parasitologists can diagnose C. sinensis through DNA testing on internal transcribed spacer (ITS: Xiao et al 2013;Tatonova et al 2017), nicotinamide adenine dinucleotide hydrogen dehydrogenase (NAD) (Sun et al 2013;Xiao et al 2013), and cytochrome c oxidase subunits (CO: Sun et al 2013). Molecular tests reveal that C. sinensis is genetically distinct from other trematodes (Fig.…”

Section: Ancient Dna Analysesmentioning

confidence: 99%

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Archaeoparasitology of Korean Mummies

Seo¹,

Hong²,

Reinhard³

et al. 2020

The Handbook of Mummy Studies

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Archaeoparasitology is now a rapidly progressing research field, providing comprehensive context about parasitism of the past and the origins and evolution of each parasite species. As in other continents, historical parasitic infection patterns in East Asia have been successfully reconstructed by archaeoparasitological research. By interdisciplinary collaboration with archaeologists, parasitologists in South Korea also have achieved meaningful academic progress in studies on various specimens uncovered at archaeological sites. Among them, coprolites of Korean mummies of the Joseon Dynasty period (1392-1910 CE) have been the

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Section: Ancient Dna Analysesmentioning

confidence: 99%

“…Molecular tests reveal that C. sinensis is genetically distinct from other trematodes (Fig. 5) (Xiao et al 2013;Tatonova et al 2017;Hong et al 2019c). Archaeoparasitologists also have tried to reveal the paleogenetics of C. sinensis by studies on mummy samples retrieved from archeological sites.…”

Section: Ancient Dna Analysesmentioning

confidence: 99%

Archaeoparasitology of Korean Mummies

Seo¹,

Hong²,

Reinhard³

et al. 2020

The Handbook of Mummy Studies

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“…Although these aspects of the epidemiology of clonorchiasis (transmission and prevalence) are relatively well understood [4,8], there is little comprehensive information on the genetics of C. sinensis and what impact genetic variation within and among populations has on the biology and transmission of C. sinensis infection and the severity of clonorchiasis and/or associated cancer. Published population genetic studies conducted using selected genetic markers, including the internal transcribed spacers (ITS-1 and/or ITS-2) of nuclear ribosomal DNA and the mitochondrial cox1 gene regions, have indicated variable levels of genetic variation and sub-structuring within C. sinensis populations [13][14][15][16][17][18][19][20][21][22]. Other studies have reported draft nuclear genomes of C. sinensis from Korea and China [23,24] and four mitochondrial genomes of individual isolates from Korea, China and the Russian Far East [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Marked mitochondrial genetic variation in individuals and populations of the carcinogenic liver fluke Clonorchis sinensis

et al. 2020

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Clonorchiasis is a neglected tropical disease caused by the Chinese liver fluke, Clonorchis sinensis, and is often associated with a malignant form of bile duct cancer (cholangiocarcinoma). Although some aspects of the epidemiology of clonorchiasis are understood, little is known about the genetics of C. sinensis populations. Here, we conducted a comprehensive genetic exploration of C. sinensis from endemic geographic regions using complete mitochondrial protein gene sets. Genomic DNA samples from C. sinensis individuals (n = 183) collected from cats and dogs in China (provinces of Guangdong, Guangxi, Hunan, Heilongjiang and Jilin) as well as from rats infected with metacercariae from cyprinid fish from the Russian Far East (Primorsky Krai region) were deep sequenced using the BGISEQ-500 platform. Informatic analyses of mitochondrial protein gene data sets revealed marked genetic variation within C. sinensis; significant variation was identified within and among individual worms from distinct geographical locations. No clear affiliation with a particular location or host species was evident, suggesting a high rate of dispersal of the parasite across endemic regions. The present work provides a foundation for future biological, epidemiological and ecological studies using mitochondrial protein gene data sets, which could aid in elucidating associations between particular C. sinensis genotypes/haplotypes and the pathogenesis or severity of clonorchiasis and its complications (including cholangiocarcinoma) in humans.

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“…However, the small number of polymorphisms in the ITS sequence (one locus) does not necessarily mean that there was no genetic differentiation. Analysis of the rDNA region has provided new data that will be useful for future studies addressing the molecular evolution, biology, and ecology of C. sinensis (Tatonova et al, 2017). Indeed, due to selective pressures from the human host, many parasites, such as Giardia species, have low levels of genetic variation.…”

Section: Discussionmentioning

confidence: 99%

“…Nuclear ribosomal DNA (rDNA) is widely used for molecular investigations and genetic analyses (Park, 2007). Ribosomal genes, including 18S, 5.8S and 28S rRNA, are typically organized into tandem repeats separated by two internal transcribed spacers (ITS1 and ITS2) (Prasad et al, 2008; Tatonova et al, 2017). Due to their high interspecific and low intraspecific variability, the ITS1 and ITS2 genes have been used extensively for ecological genetic studies and phylogenetic and evolutionary analyses at various taxonomic levels for different organisms (Dai et al, 2012; Buathong et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification and Phylogenetic Analysis of Nuclear rDNA Sequences of Clonorchis sinensis Isolates From Human Fecal Samples in Heilongjiang Province, China

et al. 2019

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Studying the genetic diversity of parasite is important for understanding their biogeography and molecular epidemiology, as well as for establishing disease prevention and control strategies. Clonorchis sinensis is an important foodborne parasite worldwide. However, despite its epidemiological significance, the genetic diversity of C. sinensis has not been well studied from human in northeastern China. In this study, a total of 342 fecal specimens were collected from residents living in five villages in Heilongjiang Province and analyzed for the presence of C. sinensis by PCR amplification and sequencing of the internal transcribed spacer 1 (ITS1) and ITS2 regions of nuclear ribosomal DNA. 21.64% (74/342) of fecal samples were found to be positive for C. sinensis by PCR. The sequences of the ITS1 region in 34 of the 74 samples (45.95%) matched that of MK179278, Genetic polymorphisms were observed at six nucleotide sites. The ITS2 gene sequence of 37 of the 74 samples (50%) matched that of MK179281. In conclusion, a low degree of genetic diversity between C. sinensis isolates from China and different geographical regions was found at ITS loci. Despite this conservation, sequencing of the rDNA region has provided important data that will be useful for future studies addressing the molecular evolution, biology, medical implications and ecology of C. sinensis.

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Inter-individual and intragenomic variations in the ITS region of Clonorchis sinensis (Trematoda: Opisthorchiidae) from Russia and Vietnam

Cited by 15 publications

References 52 publications

Archaeoparasitology of Korean Mummies

Archaeoparasitology of Korean Mummies

Marked mitochondrial genetic variation in individuals and populations of the carcinogenic liver fluke Clonorchis sinensis

Molecular Identification and Phylogenetic Analysis of Nuclear rDNA Sequences of Clonorchis sinensis Isolates From Human Fecal Samples in Heilongjiang Province, China

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