Inter‐animal variation and its influence on the overall precision of morphometric estimates based on nested sampling designs

Gupta, Madhu; Mayhew, Terry M.; Bedi, K. S.; Sharma, Ashutosh; White, F. H.

doi:10.1111/j.1365-2818.1983.tb04241.x

Cited by 64 publications

(37 citation statements)

References 15 publications

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“…The high variability in the measurements of the organ morphometry in the present study was in line with the conclusion of a prior study that biological variation between individuals is likely to be the major factor influencing the overall precision and efficiency of nested sampling schemes for morphometric analysis of thin sections [21]. Therefore, it was recommended that it is important to examine more animals to reduce the variance of the group mean [21].…”

Section: Discussionsupporting

confidence: 75%

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Morphometry of Organs in Sheep Fed a Diet Containing Tannins and Polyphenols

Mahgoub¹,

Inwa

Kadim³

et al. 2017

SQU Journal for Science

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ABSTRACT:The aim of this study was to use morphometry to investigate the essential organs of sheep fed with nonconventional diets. Twelve male Omani sheep were fed with one of two types of roughages supplemented with a commercial concentrate for 63 days and then slaughtered. The volumes of their kidneys, testes, and livers were measured. Random samples from the organs were fixed in formalin and embedded in paraffin. Random five micrometer sections from each block were stained with hematoxylin and eosin. Images from four random fields were taken from each slide using a digital camera attached to the microscope and then loaded onto a stereology software installed on a personal computer. Morphometric measurements of body organs were taken. Animals fed diets containing tannins had lower final total body weight and lower weights and volumes of their livers, testes and kidneys. There was high variability in the results of intestinal parts measurements, but there was a general trend of the treated animals having higher duodenal and ilial height, villus height, crypt depth and epithelial thickness. The only significant diet effect was higher liver l and lower Vfrac cytoplasm in the control animals than in those treated. The only significant treatment effect on the kidney measurements was a higher tube length/density in treated animals. There were no treatment effects on the testes measurements. The current study indicates that sheep may safely utilize diets containing the levels of polyphenols and tannins used here with no drastic effects on the digestive system and vital organs.

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Section: Discussionsupporting

confidence: 75%

“…Therefore, it was recommended that it is important to examine more animals to reduce the variance of the group mean [21].…”

Section: Discussionmentioning

confidence: 99%

Morphometry of Organs in Sheep Fed a Diet Containing Tannins and Polyphenols

Mahgoub¹,

Inwa

Kadim³

et al. 2017

SQU Journal for Science

View full text Add to dashboard Cite

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“…Sampling is efficient when adequate precision is achieved with reasonable effort and cost. Stereological methods that yield maximum accuracy and efficiency with reasonable precision should be selected (38)(39)(40).…”

Section: Stereologic Methodsmentioning

confidence: 99%

An Official Research Policy Statement of the American Thoracic Society/European Respiratory Society: Standards for Quantitative Assessment of Lung Structure

Hsia¹,

Hyde²,

Ochs³

et al. 2010

Am J Respir Crit Care Med

772

695

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“…This phenomenon has been elegantly discussed in a paper by Gundersen and Osterby [18] Do More Less Well! and by Gupta et al [19].…”

Section: Discussionmentioning

confidence: 99%

Measuring the Volume Density of the Glomerular Mesangium

Rich

et al. 1988

Nephron

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Volume density (V_v) is a morphometric parameter used to detect pathological changes within renal tissue. To establish the most efficient technique, V_v of the mesangium in renal biopsies was studied. First, a digitizing tablet was used to trace mesangial and total glomerular areas, subsequently used to calculate V_v of the mesangium. Secondly, a point counting technique was tested using either a 16-point or an 81-point grid; the number of grid points falling on the mesangium and glomerulus was used to calculate mesangial V_v. The digitizing tablet, the 81-point grid and the 16-point grid all gave equivalent V_v for the 15 biopsies. The 81-point grid and the 16-point grid were, respectively, 1.9 and 3.2-fold faster than the digitizing tablet. Since all techniques gave comparable results, the 16-point grid was the most efficient.

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Inter‐animal variation and its influence on the overall precision of morphometric estimates based on nested sampling designs

Cited by 64 publications

References 15 publications

Morphometry of Organs in Sheep Fed a Diet Containing Tannins and Polyphenols

Morphometry of Organs in Sheep Fed a Diet Containing Tannins and Polyphenols

An Official Research Policy Statement of the American Thoracic Society/European Respiratory Society: Standards for Quantitative Assessment of Lung Structure

Measuring the Volume Density of the Glomerular Mesangium

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