Intensive Poultry Farming Practices Influence Antibiotic Resistance Profiles in Pseudomonas aeruginosa Inhabiting Nearby Soils

Kousar, Shaheena; Rehman, Nikhar; Javed, Asghar; Hussain, Aamir; Naeem, Muhammad; Masood, Samina; Ali, Hafiz Arslan; Manzoor, Amina; Khan, Aleem Ahmed; Akrem, Ahmed; Iqbal, Fatima; Zulfiqar, Aqsa; Jamshaid, Muhammad; Waqas, Muhammad; Waseem, Asma; Saeed, Muhammad Qamar

doi:10.2147/idr.s324055

Cited by 12 publications

(12 citation statements)

References 17 publications

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“…Concerning the antimicrobial susceptibility testing, the retrieved P. aeruginosa strains were highly resistant to several antimicrobial classes including aminoglycosides, tetracycline, sulfonamides, penicillin, cephalosporins, macrolides, and β-Lactam-β-lactamase-inhibitor combination. These findings nearly agreed with those recorded by Kousar 40 and Mohamed. 41 On the other hand, norfloxacin and colistin-sulfate exhibited optimistic antimicrobial activity toward the tested P. aeruginosa strains recovered from broiler chicken.…”

supporting

confidence: 92%

oprL Gene Sequencing, Resistance Patterns, Virulence Genes, Quorum Sensing and Antibiotic Resistance Genes of XDR Pseudomonas aeruginosa Isolated from Broiler Chickens

Algammal¹,

Eidaroos²,

Alfifi³

et al. 2023

IDR

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Background: Pseudomonas aeruginosa is incriminated in septicemia, significant economic losses in the poultry production sector, and severe respiratory infections in humans. This study aimed to investigate the occurrence, oprL sequencing, antimicrobial resistance patterns, virulence-determinant, Quorum sensing, and antibiotic resistance genes of P. aeruginosa retrieved from broiler chickens. Methods: Two hundred samples were collected from 120 broiler chickens from broiler farms at Ismailia Governorate, Egypt. Consequently, the bacteriological examination was conducted and the obtained P. aeruginosa strains were tested for oprL gene sequencing, antibiogram, and PCR screening of virulence, Quorum sensing, and antibiotic resistance genes. Results: The overall prevalence of P. aeruginosa in the examined birds was 28.3%. The oprL gene sequence analysis underlined that the tested strain expressed a notable genetic identity with various P. aeruginosa strains isolated from different geographical areas in the USA, India, China, Chile, and Ghana. PCR evidenced that the obtained P. aeruginosa strains, carrying virulence-related genes: oprL, toxA, aprA, phzM, and exoS in a prevalence of 100%, 100%, 42.5%, 33.3%, and 25.9%, respectively. Moreover, the recovered P. aeruginosa strains possessed the Quorum sensing genes: lasI, lasR, rhlI, and rhlR in a prevalence of 85.2%, 85.2%, 81.5%, and 81.5%, respectively. Furthermore, 40.7% of the isolated P. aeruginosa were XDR to seven antimicrobial classes, possessing sul1, bla TEM , tetA, bla CTX-M , bla OXA-1 , and aadA1 genes. Conclusion:As we can tell, this is the first report emphasizing the evolution of XDR P. aeruginosa strains from broiler chicken in Egypt, which is supposed to be a serious threat to public health. The emerging XDR P. aeruginosa in poultry frequently harbored the oprL, toxA, and aprA virulence genes, the lasI, lasR, rhlI, and rhlR Quorum sensing genes, and the sul1, bla TEM , tetA, bla CTXM , bla OXA-1 , and aadA1 resistance genes.

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confidence: 92%

oprL Gene Sequencing, Resistance Patterns, Virulence Genes, Quorum Sensing and Antibiotic Resistance Genes of XDR Pseudomonas aeruginosa Isolated from Broiler Chickens

Algammal¹,

Eidaroos²,

Alfifi³

et al. 2023

IDR

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“…Globally, the use of antibiotics in both layer and broiler production systems has been reported [ 6 , 44 , 45 ]. Most antibiotics used in poultry production are also used in humans and share the same drug classification, mechanisms of action, and side-effects [ 46 ].…”

Section: Introductionmentioning

confidence: 99%

“…Some antibiotics currently used include tetracycline, amoxicillin, erythromycin, gentamicin, doxycycline, sulfadimidine, and sulfamethoxazole/ trimethoprim and are usually accessed without prescriptions [ 47 ]. Unfortunately, many poultry microorganisms have developed resistance to these antibiotics [ 3 , 45 , 48 ]. Similar information has been reported among African countries [ 3 , 29 , 48 , 49 , 50 ] and this generally calls for more prudent use of antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Knowledge, Attitudes, and Practices of Community Pharmacy Professionals on Poultry Antibiotic Dispensing, Use, and Bacterial Antimicrobial Resistance in Zambia: Implications on Antibiotic Stewardship and WHO AWaRe Classification of Antibiotics

et al. 2022

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Globally, the inappropriate dispensing and use of antibiotics in animals has contributed to the development of bacterial antimicrobial resistance (AMR). In Zambia, there is insufficient information among community pharmacy professionals on antibiotic use (ABU) and AMR in food-producing animals. This study assessed community pharmacy professionals’ knowledge, attitudes, and practices regarding poultry antibiotic dispensing, use, and bacterial AMR in the Lusaka district of Zambia. A cross-sectional study was conducted among 178 community pharmacy professionals between February and April 2022 using a semi-structured questionnaire. Data were analyzed using Stata version 17. Of the total participants (n = 178), 51.1% (n = 91) were pharmacists. The most dispensed antibiotic was oxytetracycline, a Watch antibiotic, mainly without prescriptions. Good knowledge of ABU and AMR was associated with work experience for more than one year (p = 0.016), while good practices were associated with male gender (p = 0.039) and work experience of more than one year (p = 0.011). The study found moderate knowledge, positive attitudes, and moderate practices of pharmacy professionals on poultry ABU and AMR. There was high dispensing of poultry antibiotics without prescriptions, which calls for strict implementation of antimicrobial stewardship and surveillance programs in poultry production in Zambia to reduce AMR.

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“…This growing trend is changing animal production practices, and local farms are promptly being replaced by market-oriented, large-scale, intensive livestock systems [1]. To meet the global population's food requirements, antibiotics have become an essential component in animal husbandry, being extensively used not only for therapeutic aims but also for preventing disease as prophylactic drugs and as growth promoters [2,3]. Despite the benefits of treating animal diseases using antibiotics, their excessive use has generated a higher selective pressure that has contributed to the current antibiotic resistance emergence.…”

Section: Introductionmentioning

confidence: 99%

Potential Applications of Essential Oils for Environmental Sanitization and Antimicrobial Treatment of Intensive Livestock Infections

et al. 2022

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The extensive use of antibiotics has contributed to the current antibiotic resistance crisis. Livestock infections of Salmonella spp, Clostridium spp. and E. coli antimicrobial-resistant bacteria represent a public threat to human and animal health. To reduce the incidence of these zoonoses, essential oils (EOs) could be effective antibiotic alternatives. This study aims at identifying EOs safe for use, effective both in complementary therapy and in the environmental sanitization of intensive farming. Natural products were chemo-characterized by gas chromatography. Three S. Typhimurium, three C. perfringens and four E. coli strains isolated from poultry and swine farms were used to assess the antimicrobial properties of nine EOs and a modified GR-OLI (mGR-OLI). The toxicity of the most effective ones (Cinnamomum zeylanicum, Cz; Origanum vulgare, Ov) was also evaluated on porcine spermatozoa and Galleria mellonella larvae. Cz, Ov and mGR-OLI showed the strongest antimicrobial activity; their volatile components were also able to significantly inhibit the growth of tested strains. In vitro, Ov toxicity was slightly lower than Cz, while it showed no toxicity on G. mellonella larvae. In conclusion, the study confirms the importance of evaluating natural products to consolidate the idea of safe EO applications in reducing and preventing intensive livestock infections.

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Intensive Poultry Farming Practices Influence Antibiotic Resistance Profiles in Pseudomonas aeruginosa Inhabiting Nearby Soils

Cited by 12 publications

References 17 publications

oprL Gene Sequencing, Resistance Patterns, Virulence Genes, Quorum Sensing and Antibiotic Resistance Genes of XDR Pseudomonas aeruginosa Isolated from Broiler Chickens

oprL Gene Sequencing, Resistance Patterns, Virulence Genes, Quorum Sensing and Antibiotic Resistance Genes of XDR Pseudomonas aeruginosa Isolated from Broiler Chickens

Knowledge, Attitudes, and Practices of Community Pharmacy Professionals on Poultry Antibiotic Dispensing, Use, and Bacterial Antimicrobial Resistance in Zambia: Implications on Antibiotic Stewardship and WHO AWaRe Classification of Antibiotics

Potential Applications of Essential Oils for Environmental Sanitization and Antimicrobial Treatment of Intensive Livestock Infections

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