Intensifying aqueous two-phase extraction by adding decisive excipients for enhancement of stability and solubility of biomolecules

Wessner, Maximilian; Meier, Marc; Bommarius, Bettina; Bommarius, Andreas S.; Brandenbusch, Christoph

doi:10.1016/j.cep.2021.108534

Cited by 4 publications

(2 citation statements)

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“…13 Nowadays, alcohol-based ATPS is more economically viable than IL-based ATPS because of the lower cost of alcohol and the ease of back-extraction of biomolecules from the alcohol-rich phase via evaporation. 14,15 This diversity of combinations allows the application of these systems in the partition and separation of various molecules, such as rutin, 16 caffeine, 17 flavones and sugars, 18 protein, 19 paracetamol, 20 lipase, 21 protease, 22 antibiotics 23 and Immunoglobulin G. 24 ATPSs usually have a neutral or slightly alkaline pH, 25 which is incompatible with a set of molecules that are soluble in aqueous solutions with a basic pH, such as humic acid, ibuprofen and paracetamol, 26 demonstrating the importance of constructing diagrams formed by hydroxides such as lithium (LiOH), sodium (NaOH) and potassium (KOH). Humic acids (HAs) belong to the family of humic substances (HS), produced by secondary reactions from the natural decomposition of plant and animal residues, resulting in a complex structure and relatively high molecular mass.…”

Section: Introductionmentioning

confidence: 99%

“…Nowadays, alcohol‐based ATPS is more economically viable than IL‐based ATPS because of the lower cost of alcohol and the ease of back‐extraction of biomolecules from the alcohol‐rich phase via evaporation 14,15 . This diversity of combinations allows the application of these systems in the partition and separation of various molecules, such as rutin, 16 caffeine, 17 flavones and sugars, 18 protein, 19 paracetamol, 20 lipase, 21 protease, 22 antibiotics 23 and Immunoglobulin G 24 …”

Section: Introductionmentioning

confidence: 99%

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Partition of humic acids using alkaline aqueous two‐phase systems constituted of alcohol and hydroxides

Vieira,

Buarque,

Gomes

et al. 2024

J of Chemical Tech & Biotech

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BACKGROUNDHumic acids (HAs) are macromolecules classified within the group of humic substances that are mainly related to the agricultural and environmental sectors, but they have attracted interest from the medicine, pharmaceutical, and cosmetic industries. However, these latter sectors depend on developing extraction and purification technologies for adequate use. Thus, this paper aims to study the partitioning and recovery of HA using alkaline two‐phase aqueous systems (ATPSs) based on alkali metal‐based hydroxides and alcohols. Initially, the influence of alcohols (methanol, ethanol, 1‐propanol, and 2‐propanol) and hydroxides (NaOH, KOH, and LiOH) in the phase diagrams construction was addressed.RESULTSThe phase formation is correlated to an increase in the hydrophobicity of alcohols and hydroxides, which allows for the enlargement of the biphasic region. In the proposed systems, HA is always partitioned to the hydroxide‐rich phase. Increasing the hydrophobicity of the alcohol favors the migration of HA to the opposite phase, while increasing the hydrophilicity of the hydroxides leads to better partition and recovery values. It was observed that increasing TLL favors the recovery of HA. Furthermore, there was a decrease in alcohol concentration in the hydroxide‐rich phase with an increase in the alkyl chain and TLL, improving the recovery. Finally, the best reached‐recovery value was 98.18 ± 0.41% (KHA = 0.038 ± 0.02) for ATPS formed by 1‐propanol + NaOH with TLL ≈ 55 at 298 K.CONCLUSIONSThe alkaline systems can be a novel route for recovering humic substances from the environment.This article is protected by copyright. All rights reserved.

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