Aqueous biphasic systems: A robust platform for green extraction of biomolecules

Saha, Nimai Chandra; Sarkar, Baidurya; Sen, Kamalika

doi:10.1016/j.molliq.2022.119882

Cited by 20 publications

(18 citation statements)

References 109 publications

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“…Furthermore, the distribution coefficients and extraction percentages of nicotine are observed to increase with the concentration of organic salts and TLL. Here, it can be argued that from the high values of K and E, all the organic salts provide very large distribution coefficients and extraction percentages as the inorganic salts, 20 indicating that these organic salts as salting-out media appear to perform very well in the extraction of nicotine from water.…”

Section: Phase Diagramsmentioning

confidence: 98%

“…Compared to traditional solvent extraction using environmentally unfriendly organic solvents, − salting-out technology has been found to be one of the promising sustainable treatments of hydrophilic target from its aqueous solution with several remarkable advantages, such as high extraction efficiency, low cost, the easiness of operation, and scale-up. − Lopes et al and Grozdanic et al observed that adding very small quantities of inorganic salts or ionic liquids to nicotine solutions induced a remarkable aqueous two-phase system (ATPS) formation. In our previous work, a systematic examination of the salting-out characteristics for recovering nicotine was obtained using six different inorganic salts including sodium chloride, potassium chloride, sodium bromide, potassium bromide, sodium carbonate, and sodium sulfate, and phase diagrams have been obtained for each ATPS system involved.…”

Section: Introductionmentioning

confidence: 99%

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Performance of Organic Salts as Salting-Out Media on Recovering Nicotine from Water

et al. 2023

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Three degradable organic salts, sodium formate (NaCHO 2 ), sodium succinate (Na 2 C 4 H 4 O 4 ), and sodium citrate (Na 3 C 6 H 5 O 7 ), were evaluated for the extraction of nicotine from aqueous solutions by forming two-phase systems. The detailed phase diagrams, including the binodal curves and tie-lines, were experimentally determined at 298.15 K and atmospheric pressure by the cloud point method. The three empirical equations including Merchuk, Pirdashti, and Hu models gave a perfect correlation of the experimental binodal data, and the reliability of liquid−liquid equilibrium data was evaluated using the Othmer− Tobias and Bancroft equations. It could be found that all adopted organic salts promoted the expansion of two-phase regions and had a high partition yield for nicotine extraction from water. The relative tendency of the anions to form aqueous two-phase systems being C 6 H 5 O 7 3− > C 4 H 4 O 4 2− > CHO 2 − was consistent with their valence order, which could be explained by the Gibbs hydration free-energy theory. Furthermore, the salting-out ability of salt anions was also in accordance with the parameter order (Na 3 C 6 H 5 O 7 > Na 2 C 4 H 4 O 4 > NaCHO 2 ) of the calculated effective excluded volume. Increasing the temperature from 298.15 to 318.15 K and the pH value from 8.0 to 11.0 at 298.15 K was conducive to the two-phase formation of the ternary nicotine + Na 3 C 6 H 5 O 7 + water system to increase the distribution coefficients and recovery percentages of nicotine. Computational thermodynamics of cloud point indicated that the more negative Gibbs free energy with temperature promoted the desolvation of nicotine to migrate preferentially to the top phase. It was concluded that degradable organic salts would be effective for nicotine extraction at an industrial scale.

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Section: Phase Diagramsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Performance of Organic Salts as Salting-Out Media on Recovering Nicotine from Water

et al. 2023

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“…4 One of the biggest challenges for the extraction and purification of biomolecules such as amino acids, enzymes, antioxidants, hormones, antibodies, and vitamins is the preservation of their bioactivity and intrinsic value (biocompatibility), which makes processes with green solvents and mild extractive conditions almost mandatory. Moreover, other relevant issues include the continuous operation and commercial viability of extractions at large scale (industrial feasibility), 5 which is favored by high-value products and cheap raw materials.…”

Section: Introductionmentioning

confidence: 99%

Liquid–Liquid Extraction of Some Biomolecules in the ATPS {Ethyl Lactate (1) + Sodium Potassium Tartrate (2) + Water (3)} at 298.15 K and 0.1 MPa

Velho,

Macedo

2023

J. Chem. Eng. Data

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The mounting demand for biomolecules in industry, particularly in regard to antioxidant-rich food supplements and monoclonal antibodies, has been compelling pharmaceutical companies to invest in this field and to redesign the production of active pharmaceutical ingredients (APIs) by implementing the principles of green chemistry and circular economy. For example, by promoting the use of renewable feedstocks, such as biowaste from the food sector, it is possible to simultaneously avoid the footprint of chemical synthesis and reduce the price of raw materials. Aqueous two-phase systems (ATPS), or aqueous biphasic systems (ABS), have been gaining relevance in the extraction of biomolecules such as proteins, vitamins, and lipids, given their low price, low energy demand, large scale-up potential, and considerable biocompatibility. These features make ATPS very powerful primary concentration steps in industrial processes, which become especially eco-friendly when combined with green solvents such as ethyl lactate and salts of organic basis. Among some common biomolecules in biowaste, cyanocobalamin (Cya), epicatechin (Epi), folic acid (Fol), and syringic acid (Syr) possess very interesting properties given their relatively large presence and strong antioxidant potential. Therefore, in this work, these four well-known biomolecules were extracted in the ATPS {ethyl lactate (1) + sodium potassium tartrate (2) + water (3)} at 298.15 K and 0.1 MPa. This eco-friendly ATPS allowed low mass losses (< 3 %) in the liquid−liquid extractions, having attained the maximum partition coefficients (K = 6.5 ± 0.4) and extraction efficiencies (E = 90.7 ± 0.2 %) for cyanocobalamin using the longest tie-line (TLL = 70.73 m%). Conversely, for the same tie-line composition, the smallest performance indicators (K = 1.25 ± 0.03 and E = 69.3 ± 0.2 %) were obtained for folic acid. The partition studies were performed after careful evaluation of the effect of pH on the mean electrical charge of each biomolecule.

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“…Being a water-based biocompatible system, the ABS is used for separation and purifications of biomolecules . A comprehensive review of extraction and separation of biomolecules using polymer-, IL-, and inorganic salt-based ABSs has recently been reported . Not only for biomolecule purifications, extraction and separations of different metal species were also investigated using different ABSs during the past few years.…”

Section: Introductionmentioning

confidence: 99%

“…5 A comprehensive review of extraction and separation of biomolecules using polymer-, IL-, and inorganic salt-based ABSs has recently been reported. 11 Not only for biomolecule purifications, extraction and separations of different metal species were also investigated using different ABSs during the past few years. Speciesdependent extraction of molybdenum was studied using a triblock copolymer-and sodium sulfate-based ABS.…”

Section: Introductionmentioning

confidence: 99%

Carbohydrate-Tailored PPG#400 vs Ionic Liquid-Based Aqueous Biphasic System That Enables Extraction of Polyphenols

Das,

Sen

2023

J. Chem. Eng. Data

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Polypropylene glycol (PPG#400) and an ionic liquid (IL), tris(2-hydroxyethyl)methyl ammonium methylsulfate-based aqueous biphasic system (ABS) has been constructed. The high concentration of the IL as one of the phase-forming components can make the ABS toxic and at the same time expensive. Thus, to decrease the phase-forming concentration of the IL, carbohydrates have been introduced as adjuvants. By tailoring the ABS with the help of carbohydrates, a dramatic decrease in the phase-forming concentration of IL was observed. The required concentration of the IL reduced from 80% (w/w) to ≤20% (w/w) to form an ABS with PPG#400. These observations are justified on the basis of theoretical calculations. The ABS tailored with D-mannitol as an adjuvant was chosen for further applications. The present work reports on the use of the newly designed ABS toward extraction of polyphenols such as rutin and morin. ∼75% of rutin and ∼91% of morin were extracted in the PPG#400-rich phase of ABS.

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Aqueous biphasic systems: A robust platform for green extraction of biomolecules

Cited by 20 publications

References 109 publications

Performance of Organic Salts as Salting-Out Media on Recovering Nicotine from Water

Performance of Organic Salts as Salting-Out Media on Recovering Nicotine from Water

Liquid–Liquid Extraction of Some Biomolecules in the ATPS {Ethyl Lactate (1) + Sodium Potassium Tartrate (2) + Water (3)} at 298.15 K and 0.1 MPa

Carbohydrate-Tailored PPG#400 vs Ionic Liquid-Based Aqueous Biphasic System That Enables Extraction of Polyphenols

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