Carbohydrate-Tailored PPG#400 vs Ionic Liquid-Based Aqueous Biphasic System That Enables Extraction of Polyphenols

Abstract

Polypropylene glycol (PPG#400) and an ionic liquid (IL), tris(2-hydroxyethyl)methyl ammonium methylsulfate-based aqueous biphasic system (ABS) has been constructed. The high concentration of the IL as one of the phase-forming components can make the ABS toxic and at the same time expensive. Thus, to decrease the phase-forming concentration of the IL, carbohydrates have been introduced as adjuvants. By tailoring the ABS with the help of carbohydrates, a dramatic decrease in the phase-forming concentration of IL was observed. The required concentration of the IL reduced from 80% (w/w) to ≤20% (w/w) to form an ABS with PPG#400. These observations are justified on the basis of theoretical calculations. The ABS tailored with D-mannitol as an adjuvant was chosen for further applications. The present work reports on the use of the newly designed ABS toward extraction of polyphenols such as rutin and morin. ∼75% of rutin and ∼91% of morin were extracted in the PPG#400-rich phase of ABS.

Partition of Chlorogenic and Nicotinic Acids Using Eco-Friendly ATPSs Containing Ethyl Lactate at 298.15 K and 0.1 MPa

“…Aqueous two-phase systems (ATPSs) constitute a liquid–liquid extraction technique with low environmental impact which has been greatly applied in the recovery of biomolecules such as proteins, − vitamins, − and antioxidants, − generally providing extraction media with low interfacial tension and toxicity, high biocompatibility, and scale-up potential . Therefore, these systems could provide a promising alternative for the extraction of polyphenols without damaging their bioactivity …”