Intensified extraction of ionized natural products by ion pair centrifugal partition extraction

Stepwise Elution of a Three‐phase Solvent System in Centrifugal Partition Extraction: A New Strategy for the Fractionation and Phytochemical Screening of a Crude Bark Extract

“…In our operating conditions, the initial stationary phase retention was 68% when working at 20 mL/min after loading 5 g of the crude bark extract of A. leiocarpus . This value was certainly good with respect to the maximum (76%) that can be obtained with this instrument (the interconnecting ducts involved in the transport of the mobile phase between partition cells represent 24% of the total CPE column volume) (Hamzaoui et al ., ).…”

“…These particular features make it possible to pump the mobile phase at higher flow rates, ranging from 10 to 50 mL/min, while maintaining a good hydrodynamic stability of the liquid phases inside the column. Until now, separation procedures using CPE have been investigated only for the purification of ionic compounds when combined to the ion‐exchange displacement mode (Hamzaoui et al ., ). Two recent articles dedicated to the purification of glycyrrhizin from licorice root and of sinalbin from white mustard seeds have demonstrated that the introduction of chemical exchange sites in the liquid stationary phase results in the recovery of highly pure ionic compounds (> 95%) (Hamzaoui et al ., , ).…”

“…Until now, separation procedures using CPE have been investigated only for the purification of ionic compounds when combined to the ion‐exchange displacement mode (Hamzaoui et al ., ). Two recent articles dedicated to the purification of glycyrrhizin from licorice root and of sinalbin from white mustard seeds have demonstrated that the introduction of chemical exchange sites in the liquid stationary phase results in the recovery of highly pure ionic compounds (> 95%) (Hamzaoui et al ., , ). In other development modes, such as elution, it is possible that the small number of partition cells in the CPE rotor could decrease the number of unit transfers and thus drastically reduce the process efficiency and thus the separation resolution.…”

Concentration and selective fractionation of an antihypertensive peptide from an alfalfa white proteins hydrolysate by mixed ion-exchange centrifugal partition chromatography

“…Nevertheless, the weak point of this protocol is the run duration, which affects the process productivity. This problem should be overcome by transposing the MIXCPC process on a support-free liquid-liquid device with less partition cells, such as the recently developed centrifugal partition extractor FCPE ® [24,25]. The purity of the fraction of interest could be increased, if necessary, by the subsequent use of a technique having an orthogonal selectivity criterion such as preparative reversed phase HPLC.…”

Intensified Separation of Steviol Glycosides from a Crude Aqueous Extract of Stevia rebaudiana Leaves Using Centrifugal Partition Chromatography

“…In this study, the FCPE300 ® column capacity was 303 mL (instead of 250 mL for the CPC experiments), comprising 231 twin-cells of 1 mL each (instead of 0.1 mL). It was demonstrated previously that the FCPE300 ® instrument is of great interest for the large-scale purification of ionized compounds when the ion exchange mode is used [33,35]. The objective here was to investigate if this instrument can also be efficient for the separation of structurally close molecular structures when working in the elution mode.…”