Intensified crude glycerol conversion to butanol by immobilized Clostridium pasteurianum

Krasňan, Vladimír; Plž, Michal; Marr, Andrew C.; Markošová, Kristína; Rosenberg, Michal; Rebroš, Martin

doi:10.1016/j.bej.2018.03.005

Cited by 24 publications

(9 citation statements)

References 30 publications

(45 reference statements)

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“…Their uniform shape and size are tailored for high mass transport and easy separation from the reaction medium, all of which makes them interesting for continuous operation in flow reactors (Krasňan et al 2016 ). But LentiKats® production is time consuming and requires dedicated equipment such as the LentiPrinter® (Rebroš et al 2013 ; Krasňan et al 2018 ). Therefore, for now only the PAM succeeded in meeting our set aim of a straightforward encapsulation process.…”

Section: Discussionmentioning

confidence: 99%

Continuous process technology for glucoside production from sucrose using a whole cell-derived solid catalyst of sucrose phosphorylase

Kruschitz

Peinsipp

Pfeiffer

et al. 2021

Appl Microbiol Biotechnol

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Advanced biotransformation processes typically involve the upstream processing part performed continuously and interlinked tightly with the product isolation. Key in their development is a catalyst that is highly active, operationally robust, conveniently produced, and recyclable. A promising strategy to obtain such catalyst is to encapsulate enzymes as permeabilized whole cells in porous polymer materials. Here, we show immobilization of the sucrose phosphorylase from Bifidobacterium adolescentis (P134Q-variant) by encapsulating the corresponding E. coli cells into polyacrylamide. Applying the solid catalyst, we demonstrate continuous production of the commercial extremolyte 2-α-d-glucosyl-glycerol (2-GG) from sucrose and glycerol. The solid catalyst exhibited similar activity (≥70%) as the cell-free extract (~800 U g−1 cell wet weight) and showed excellent in-operando stability (40 °C) over 6 weeks in a packed-bed reactor. Systematic study of immobilization parameters related to catalyst activity led to the identification of cell loading and catalyst particle size as important factors of process optimization. Using glycerol in excess (1.8 M), we analyzed sucrose conversion dependent on space velocity (0.075–0.750 h−1) and revealed conditions for full conversion of up to 900 mM sucrose. The maximum 2-GG space-time yield reached was 45 g L−1 h−1 for a product concentration of 120 g L−1. Collectively, our study establishes a step-economic route towards a practical whole cell-derived solid catalyst of sucrose phosphorylase, enabling continuous production of glucosides from sucrose. This strengthens the current biomanufacturing of 2-GG, but also has significant replication potential for other sucrose-derived glucosides, promoting their industrial scale production using sucrose phosphorylase. Key points • Cells of sucrose phosphorylase fixed in polyacrylamide were highly active and stable. • Solid catalyst was integrated with continuous flow to reach high process efficiency. • Generic process technology to efficiently produce glucosides from sucrose is shown. Graphical abstract

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Section: Discussionmentioning

confidence: 99%

Continuous process technology for glucoside production from sucrose using a whole cell-derived solid catalyst of sucrose phosphorylase

Kruschitz

Peinsipp

Pfeiffer

et al. 2021

Appl Microbiol Biotechnol

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“…Butanol production from glycerol was studied in polyvinyl alcohol particles entrapped by Clostridium pasteurianum . The glycerol of different impurities from biodiesel production achieved butanol productivity of 2.90 g/L/h and 1.76 g/L/h [ 123 ]. Clostridium sp.…”

Section: Glycerol Into Value-added Productsmentioning

confidence: 99%

Glycerol waste to value added products and its potential applications

Chilakamarry

Sakinah

Zularisam

et al. 2021

Syst Microbiol and Biomanuf

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Graphic abstract The rapid industrial and economic development runs on fossil fuel and other energy sources. Limited oil reserves, environmental issues, and high transportation costs lead towards carbon unbiased renewable and sustainable fuel. Compared to other carbon-based fuels, biodiesel is attracted worldwide as a biofuel for the reduction of global dependence on fossil fuels and the greenhouse effect. During biodiesel production, approximately 10% of glycerol is formed in the transesterification process in a biodiesel plant. The ditching of crude glycerol is important as it contains salt, free fatty acids, and methanol that cause contamination of soil and creates environmental challenges for researchers. However, the excessive cost of crude glycerol refining and market capacity encourage the biodiesel industries for developing a new idea for utilising and produced extra sources of income and treat biodiesel waste. This review focuses on the significance of crude glycerol in the value-added utilisation and conversion to bioethanol by a fermentation process and describes the opportunities of glycerol in various applications.

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“…The crude glycerol produced as side stream during biodiesel production contains mainly glycerol (77–90%) followed by water (5.3–14.2%), salts (4.2–5.5% NaCl or 0.8–6.6% K 2 SO 4 ), ash (0.9–6.3%), residual methanol, mono-, di-, and tri-glycerides, free fatty acids, and other impurities depending on the raw material . The transition of the conventional biodiesel industry into a sustainable biorefinery could be based on crude glycerol valorization for the production of bio-based chemicals and polymers, such as microbial oil, 1,3-propanediol, poly (3-hydroxybutyrate), butanol, 2,3-butanediol (BDO), and succinic acid. − …”

Section: Introductionmentioning

confidence: 99%

Bioprocess Development for 2,3-Butanediol Production from Crude Glycerol and Conceptual Process Design for Aqueous Conversion into Methyl Ethyl Ketone

Maina

Dheskali²,

Papapostolou

et al. 2021

ACS Sustainable Chem. Eng.

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The transition to the circular economy era necessitates the sustainable production of chemicals using crude renewable resources via integrated biological and chemical conversion routes. Within this concept, this study combined experimental fermentation development with conceptual process design and technoeconomic evaluation to demonstrate the feasibility of bio-based methyl ethyl ketone (MEK) production from crude glycerol derived from industrial biodiesel production using soybean oil alone or mixed with tallow fat. 2,3-Butanediol (BDO) production via fermentation of crude glycerol was initially developed using a Klebsiella michiganensis strain leading to 76.1 g/L BDO concentration with a productivity of 1.38 g/(L h) at a volumetric mass transfer coefficient of 77 h–1. Direct aqueous BDO conversion into MEK followed by purification via pressure swing distillation was subsequently evaluated via conceptual process design. The bio-based MEK minimum selling price (MSP) is close to the 11 year average market price of petroleum-derived MEK ($1.8/kg) when the crude glycerol cost is up to $0.1/kg at annual MEK production capacities higher than ca. 20 000 t. Monte Carlo simulations showed ca. 30% probability of obtaining an MSP lower than $1.8/kg at a crude glycerol cost of $0.1/kg. This study demonstrated the feasibility of cost-competitive bio-based MEK production as an industrial solvent and an intermediate chemical.

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Intensified crude glycerol conversion to butanol by immobilized Clostridium pasteurianum

Cited by 24 publications

References 30 publications

Continuous process technology for glucoside production from sucrose using a whole cell-derived solid catalyst of sucrose phosphorylase

Continuous process technology for glucoside production from sucrose using a whole cell-derived solid catalyst of sucrose phosphorylase

Glycerol waste to value added products and its potential applications

Bioprocess Development for 2,3-Butanediol Production from Crude Glycerol and Conceptual Process Design for Aqueous Conversion into Methyl Ethyl Ketone

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