Intensification of the cellular accumulation of amino acids by pyridoxal

Effect of pyridoxal on the active transport of amino acids by sacs of everted intestine of the golden hamster (Mesocricetus auratus)

“…1953), while that of the hamster small intestine is lowered. It is unlikely that this difference is due to a relative deficiency of pyridoxal in the neoplastic cells, as Riggs et al (1953) found that the stimulation of glycine uptake occurred even when neoplastic cells had been previously incubated in a medium containing 0 5 mM-pyridoxal. Also, they found stimulation of active uptake of glycine by pyridoxal when neoplastic cells were grown in mice fed on a pyridoxine-fortified diet.…”

“…The results suggest that the mechanism for active transport of amino acids by the Ehrlich mouse ascites carcinoma cell differs to some extent from that of the normal hamster small intestine. At the concentrations of glycine and pyridoxal used the active transport of glycine by the neoplastic cells is greatly stimulated (Riggs et at. 1953), while that of the hamster small intestine is lowered.…”

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During the investigation of the uptake of amino acids by Ehrlich mouse ascites carcinoma cells, Riggs, Coyne & Christensen (1953) found that the degree to which glycine was concentrated could be much increased by the addition of certain quantities of pyridoxal to the suspending medium. This effect was also obtained, but to a lesser degree, when L-a, y-diaminobutyric acid and L-methionine were used, while the uptake of L-tryptophan was only stimulated when the neoplastic cells had been grown in pyridoxine-deficient mice (Christensen, Riggs & Coyne, 1954).

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Effect of deoxypyridoxine on the intestinal amino acid absorption in the chicken in situ

“…Using mouse Ehrlich ascites tumor cells in vitro, the participation of vitamin B6 or its derivatives in the active transport of amino acids was found by RIGGS et al 15) and CHRISTENSEN et at.2). FRIDHANDLER and QUASTEL5) using the isolated surviving guinea pig intestine, and JACOBS and coworkers7,8) using an in situ perfusion technique in rat intestine, also reported the participation of vitamin B6 in the active amino acid absorption from the intestine.…”

An Antihemolytic Action of Pyridoxal.

“…Evidence obtained with tumor cells from pyridoxine-deficient mice implicates pyridoxal in the normal process of amino acid accumulation by these cells (3,4). A close relationship to the transfer of potassium into cells is also indicated (3).…”