Intelligent Fusion Imaging Photonics for Real-Time Lighting Obstructions

Do, Hyeonsu; Yoon, Colin; Liu, Yunbo; Zhao, Xintao; Gregg, John M.; Da, Ancheng; Park, Younggeun; Lee, Somin Eunice

doi:10.3390/s23010323

Cited by 3 publications

(1 citation statement)

References 35 publications

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“…The size of the elliptical Airy pattern would be limited by the diffraction limit; however, we anticipate that the ellipticity and the intensity of the elliptical Airy pattern would vary due to the dependence between the scattered field amplitude and the scattering cross-section of the target. As a model candidate of an anisotropic − nanostructured target to validate the fundamental reason that we can see the ellipse Airy pattern, we simulated the scattered electric field pattern of gold nanorods (Figure a). Here, we acquired the nanostructural information from the ellipse Airy pattern by phase-intensity and phase-ellipticity.…”

Section: Resultsmentioning

confidence: 99%

Interferometric Phase Intensity Nanoscopy (iPINE), Revealing Nanostructural Features, Resolves Proximal Nanometer Objects below the Diffraction Limit: Implications in Long-Time Super-Resolution of Biological Dynamics

Cui,

Kim,

et al. 2024

ACS Appl. Nano Mater.

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The ability to spatially and temporally map nanoscale environments in situ over extended time scales would be transformative for biology, biomedicine, and bioengineering. All nanometer objects, from nanoparticles down to single proteins, scatter light. Interferometric scattering stands as a powerful tool, offering ultrasensitivity and resolution vital for visualizing nanoscale entities. Interferometric scattering from an individual nanoparticle down to an individual protein has been detected; however, resolving adjacent nanometer objects with interferometric scattering has not yet been demonstrated. In this work, we present interferometric phase intensity nanoscopy (iPINE) to resolve adjacent nanometer objects with interferometric scattering. We demonstrate that multiphase and sensitivity of iPINE reveal ellipse Airy patterns correlated with nanostructural features. We show that eliminating background fluctuation by employing circularly polarized illumination in iPINE is essential to separate proximal nanometer objects below the diffraction limit. We envision iPINE for resolving a variety of adjacent nanometer objects from nanoparticles down to proximal proteins in situ over extended time periods for a wide range of applications in biology, biomedicine, and bioengineering. We expect iPINE to be especially important in applications of biological dynamics that require extended observation times.

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Section: Resultsmentioning

confidence: 99%

Interferometric Phase Intensity Nanoscopy (iPINE), Revealing Nanostructural Features, Resolves Proximal Nanometer Objects below the Diffraction Limit: Implications in Long-Time Super-Resolution of Biological Dynamics

Cui,

Kim,

et al. 2024

ACS Appl. Nano Mater.

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Phase intensity nanoscope (PINE) opens long-time investigation windows of living matter

Cui

Liu

et al. 2023

Nat Commun

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Fundamental to all living organisms and living soft matter are emergent processes in which the reorganization of individual constituents at the nanoscale drives group-level movements and shape changes at the macroscale over time. However, light-induced degradation of fluorophores, photobleaching, is a significant problem in extended bioimaging in life science. Here, we report opening a long-time investigation window by nonbleaching phase intensity nanoscope: PINE. We accomplish phase-intensity separation such that nanoprobe distributions are distinguished by an integrated phase-intensity multilayer thin film (polyvinyl alcohol/liquid crystal). We overcame a physical limit to resolve sub-10 nm cellular architectures, and achieve the first dynamic imaging of nanoscopic reorganization over 250 h using PINE. We discover nanoscopic rearrangements synchronized with the emergence of group-level movements and shape changes at the macroscale according to a set of interaction rules with importance in cellular and soft matter reorganization, self-organization, and pattern formation.

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Optical Penetration of Shape-Controlled Metallic Nanosensors across Membrane Barriers

Chu

Krach

et al. 2023

Sensors

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Precise nanostructure geometry that enables the optical biomolecular delivery of nanosensors to the living intracellular environment is highly desirable for precision biological and clinical therapies. However, the optical delivery through membrane barriers utilizing nanosensors remains difficult due to a lack of design guidelines to avoid inherent conflict between optical force and photothermal heat generation in metallic nanosensors during the process. Here, we present a numerical study reporting significantly enhanced optical penetration of nanosensors by engineering nanostructure geometry with minimized photothermal heating generation for penetrating across membrane barriers. We show that by varying the nanosensor geometry, penetration depths can be maximized while heat generated during the penetration process can be minimized. We demonstrate the effect of lateral stress induced by an angularly rotating nanosensor on a membrane barrier by theoretical analysis. Furthermore, we show that by varying the nanosensor geometry, maximized local stress fields at the nanoparticle–membrane interface enhanced the optical penetration process by four-fold. Owing to the high efficiency and stability, we anticipate that precise optical penetration of nanosensors to specific intracellular locations will be beneficial for biological and therapeutic applications.

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Intelligent Fusion Imaging Photonics for Real-Time Lighting Obstructions

Cited by 3 publications

References 35 publications

Interferometric Phase Intensity Nanoscopy (iPINE), Revealing Nanostructural Features, Resolves Proximal Nanometer Objects below the Diffraction Limit: Implications in Long-Time Super-Resolution of Biological Dynamics

Interferometric Phase Intensity Nanoscopy (iPINE), Revealing Nanostructural Features, Resolves Proximal Nanometer Objects below the Diffraction Limit: Implications in Long-Time Super-Resolution of Biological Dynamics

Phase intensity nanoscope (PINE) opens long-time investigation windows of living matter

Optical Penetration of Shape-Controlled Metallic Nanosensors across Membrane Barriers

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