Phase intensity nanoscope (PINE) opens long-time investigation windows of living matter

Cui, Guangjie; Liu, Yunbo; Zu, Di; Zhao, Xintao; Zhang, Zhenyu; Kim, Do Young; Senaratne, Pramith; Fox, Aaron P.; Sept, David; Park, Younggeun; Lee, Somin Eunice

doi:10.1038/s41467-023-39624-w

Cited by 6 publications

(2 citation statements)

References 72 publications

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“…Here, we overcome these challenges by interferometric phase intensity nanoscopy (iPINE). By integrating a phase-intensity multilayer thin film, herein referred to as PI, with interferometric scattering, nanometer objects below the diffraction limit can be resolved. While coherent imaging superimposes amplitude and phase of electromagnetic fields, phase-intensity modulation in iPINE enables one to separate the intensity and phase such that nanometer objects are distinguished at different phases and adjacent targets can be then resolved.…”

Section: Introductionmentioning

confidence: 99%

Interferometric Phase Intensity Nanoscopy (iPINE), Revealing Nanostructural Features, Resolves Proximal Nanometer Objects below the Diffraction Limit: Implications in Long-Time Super-Resolution of Biological Dynamics

Cui,

Kim,

et al. 2024

ACS Appl. Nano Mater.

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The ability to spatially and temporally map nanoscale environments in situ over extended time scales would be transformative for biology, biomedicine, and bioengineering. All nanometer objects, from nanoparticles down to single proteins, scatter light. Interferometric scattering stands as a powerful tool, offering ultrasensitivity and resolution vital for visualizing nanoscale entities. Interferometric scattering from an individual nanoparticle down to an individual protein has been detected; however, resolving adjacent nanometer objects with interferometric scattering has not yet been demonstrated. In this work, we present interferometric phase intensity nanoscopy (iPINE) to resolve adjacent nanometer objects with interferometric scattering. We demonstrate that multiphase and sensitivity of iPINE reveal ellipse Airy patterns correlated with nanostructural features. We show that eliminating background fluctuation by employing circularly polarized illumination in iPINE is essential to separate proximal nanometer objects below the diffraction limit. We envision iPINE for resolving a variety of adjacent nanometer objects from nanoparticles down to proximal proteins in situ over extended time periods for a wide range of applications in biology, biomedicine, and bioengineering. We expect iPINE to be especially important in applications of biological dynamics that require extended observation times.

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Section: Introductionmentioning

confidence: 99%

Interferometric Phase Intensity Nanoscopy (iPINE), Revealing Nanostructural Features, Resolves Proximal Nanometer Objects below the Diffraction Limit: Implications in Long-Time Super-Resolution of Biological Dynamics

Cui,

Kim,

et al. 2024

ACS Appl. Nano Mater.

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“…In a similar context, QPI’s compatibility with AI has opened new avenues towards improved specificity in image classification 21 . As a result, QPI has been widely utilized in exploring the structural and metabolic properties of single, living cells 10 – 15 , with recent applications extending to super-resolved imaging 22 .…”

Section: Introductionmentioning

confidence: 99%

Quantitative phase imaging by gradient retardance optical microscopy

Zhang,

Sarollahi,

Luckhart

et al. 2024

Sci Rep

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Quantitative phase imaging (QPI) has become a vital tool in bioimaging, offering precise measurements of wavefront distortion and, thus, of key cellular metabolism metrics, such as dry mass and density. However, only a few QPI applications have been demonstrated in optically thick specimens, where scattering increases background and reduces contrast. Building upon the concept of structured illumination interferometry, we introduce Gradient Retardance Optical Microscopy (GROM) for QPI of both thin and thick samples. GROM transforms any standard Differential Interference Contrast (DIC) microscope into a QPI platform by incorporating a liquid crystal retarder into the illumination path, enabling independent phase-shifting of the DIC microscope's sheared beams. GROM greatly simplifies related configurations, reduces costs, and eradicates energy losses in parallel imaging modalities, such as fluorescence. We successfully tested GROM on a diverse range of specimens, from microbes and red blood cells to optically thick (~ 300 μm) plant roots without fixation or clearing.

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Sub-10nm nanoscopic imaging reveals sub-10nm cellular architectures

Cui,

Kim,

et al. 2024

Plasmonics in Biology and Medicine XXI

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Phase intensity nanoscope (PINE) is a new super-resolution method to further improve the resolution of existing techniques. PINE utilizes an integrated phase-intensity device to modulate phase differences between electric field components to distinguish nanoprobes within a diffraction-limited region. This phase-intensity separation enables continuous imaging without photobleaching. PINE achieved sub-10nm resolution of cellular structures through precise localization of populations of randomly distributed nanorod probes. The distribution of localized nanorods forms patterns of underlying structures. By defining features from probe distribution patterns and minimizing the distances of each probe to its feature projection, PINE extracts sub-10nm structural information. PINE will pave the way for new sub-10nm longterm investigations of previously unexplored material, chemical and biological dynamics.

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Phase intensity nanoscope (PINE) opens long-time investigation windows of living matter

Cited by 6 publications

References 72 publications

Interferometric Phase Intensity Nanoscopy (iPINE), Revealing Nanostructural Features, Resolves Proximal Nanometer Objects below the Diffraction Limit: Implications in Long-Time Super-Resolution of Biological Dynamics

Interferometric Phase Intensity Nanoscopy (iPINE), Revealing Nanostructural Features, Resolves Proximal Nanometer Objects below the Diffraction Limit: Implications in Long-Time Super-Resolution of Biological Dynamics

Quantitative phase imaging by gradient retardance optical microscopy

Sub-10nm nanoscopic imaging reveals sub-10nm cellular architectures

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