Integron-mediated multidrug resistance in extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolated from fecal specimens in Egypt

Elsherif, Rasha; Ismail, Dalia Kadry; Elkholy, Yasmine S; Gohar, Noha; Elnagdy, Sherif M.; ElKraly, Omnia Abdullah

doi:10.1097/01.epx.0000483165.56114.d8

Cited by 7 publications

(8 citation statements)

References 30 publications

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“…In contrary to what reported about the association of Int III with GES gene [49], current results showed that they coexist only in one isolate out of seven Int III positive isolates. Additionally, IMP gene coexisted with Int III in 42.9% of isolates which was unlike the study conducted by Elsherif et al who reported that Int III is of low occurrence with IMP gene [49].…”

Section: Plos Onecontrasting

confidence: 99%

“…Rezai et al reported that Int I was detected in 79.3% while Int II was harbored by 10.3% of isolates [48]. ESBL-producers were significantly associated with Int I (P = 0.033), similar to what reported by Elsherif et al, as Int 1 was associated with CTX-M gene (P = 0.039) [49]. In contrary to what reported about the association of Int III with GES gene [49], current results showed that they coexist only in one isolate out of seven Int III positive isolates.…”

Section: Plos Onesupporting

confidence: 75%

“…ESBL-producers were significantly associated with Int I (P = 0.033), similar to what reported by Elsherif et al ., as Int 1 was associated with CTX-M gene (P = 0.039) [ 49 ]. In contrary to what reported about the association of Int III with GES gene [ 49 ], current results showed that they coexist only in one isolate out of seven Int III positive isolates. Additionally, IMP gene coexisted with Int III in 42.9% of isolates which was unlike the study conducted by Elsherif et al .…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, IMP gene coexisted with Int III in 42.9% of isolates which was unlike the study conducted by Elsherif et al . who reported that Int III is of low occurrence with IMP gene [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

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Characterization of β-lactam resistance in K. pneumoniae associated with ready-to-eat processed meat in Egypt

Abdel-Rhman

2020

PLoS ONE

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K. pneumoniae was known as a nosocomial infection that causes human diseases. It is considered as one of the food-borne pathogens as it causes septicemia and diarrhea in humans. This study aims to characterize K. pneumoniae strains isolated from ready to eat processed meat phenotypically and genetically. Three hundred and fifty ready to eat processed meat (Luncheon-meat) samples were collected. Forty-four (12.6%) K. pneumoniae strains were isolated and bio-typed, where the majority were identified to belong to biotype B1. K1 and K2 serotypes were detected and strains were classified as hypermucoviscous K. pneumoniae (HVKP) and classic K. pneumoniae (CKP) (26 and 18 isolates, respectively). The isolates were resistant to several classes of β-lactam antibiotics, ceftazidim and cefotaxime (95.5%), cefoxitin (93.2%), ertapenem (90.9%) and amoxicillin-clavulanic acid (86.4%). They were classified as extended spectrum β-lactamases (ESBLs), AmpC or carbapenemase-producers phenotypically. Eighteen β-lactamase genes were investigated by PCR. The most prominent genes were SHV (63.6%), TEM (52.2%), CTX-M15 (50%), AMPC (47.7%), CIT-M (45.5%) and VIM (43.2%). Co-detection of β-lactam resistance genes revealed 42 gene profiles. Twenty-four isolates had the complete efflux system (AcrAB-ToƖC). Besides, Integrons (I, II, III) were detected in 20 isolates. Molecular typing by ERIC-PCR showed high genetic diversity between isolates as 34 different patterns were identified. Overall, this study confirmed the hazards posed by the presence of multiple resistance genes in the same isolate and this should not be undervalued. Besides, the horizontal transfer of plasmid harboring resistance genes between isolates in food represents potential health risks for consumers in Egypt and so the control and inhibition plans are necessary.

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Section: Plos Onecontrasting

confidence: 99%

Section: Plos Onesupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Characterization of β-lactam resistance in K. pneumoniae associated with ready-to-eat processed meat in Egypt

Abdel-Rhman

2020

PLoS ONE

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“…Enteropathogenic E. coli (EPEC), diffuse-adhering E. coli (DAEC) and enteroaggregative E. coli (EAEC) are associated with infantile diarrhea in Egyptian children where 220 enteroadherent E. coli were identified from 729 diarrheic children [ 64 ]. In another study, E. coli was isolated from 52.1% hospitalized patients and 60.4% from outpatient clinics with high degrees of resistance to ciprofloxacin and co-trimoxazole [ 65 ]. Also, there is increasing incidence of carbapenemase- and extended-spectrum β-lactamase-producing Enterobacteriaceae in dairy farms and hospital-acquired infections.…”

Section: Bacterial Zoonosismentioning

confidence: 99%

A Comprehensive Review of Common Bacterial, Parasitic and Viral Zoonoses at the Human-Animal Interface in Egypt

2017

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Egypt has a unique geographical location connecting the three old-world continents Africa, Asia and Europe. It is the country with the highest population density in the Middle East, Northern Africa and the Mediterranean basin. This review summarizes the prevalence, reservoirs, sources of human infection and control regimes of common bacterial, parasitic and viral zoonoses in animals and humans in Egypt. There is a gap of knowledge conerning the epidemiology of zoonotic diseases at the human-animal interface in different localities in Egypt. Some zoonotic agents are “exotic” for Egypt (e.g., MERS-CoV and Crimean-Congo hemorrhagic fever virus), others are endemic (e.g., Brucellosis, Schistosomiasis and Avian influenza). Transboundary transmission of emerging pathogens from and to Egypt occurred via different routes, mainly importation/exportation of apparently healthy animals or migratory birds. Control of the infectious agents and multidrug resistant bacteria in the veterinary sector is on the frontline for infection control in humans. The implementation of control programs significantly decreased the prevalence of some zoonoses, such as schistosomiasis and fascioliasis, in some localities within the country. Sustainable awareness, education and training targeting groups at high risk (veterinarians, farmers, abattoir workers, nurses, etc.) are important to lessen the burden of zoonotic diseases among Egyptians. There is an urgent need for collaborative surveillance and intervention plans for the control of these diseases in Egypt.

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Childhood diarrhoeal diseases in developing countries

Ugboko

Nwinyi

Oranusi

et al. 2020

Heliyon

137

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Diarrhoeal diseases collectively constitute a serious public health challenge globally, especially as the leading cause of death in children (after respiratory diseases). Childhood diarrhoea affecting children under the age of five accounts for approximately 63% of the global burden. Accurate and timely detection of the aetiology of these diseases is very crucial; but conventional methods, apart from being laborious and time-consuming, often fail to identify difficult-to-culture pathogens. The aetiological agent of an average of up to 40% of cases of diarrhoea cannot be identified. This review gives an overview of the recent trends in the epidemiology and treatment of diarrhoea and aims at highlighting the potentials of metagenomics technique as a diagnostic method for enteric infections.

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Integron-mediated multidrug resistance in extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolated from fecal specimens in Egypt

Abstract: ESBL-producing E. coli and K. pneumoniae showed a marked degree of antibiotic resistance in both hospitalized and nonhospitalized study groups. The high prevalence of class 1 and 2 integrons among isolates of both groups has a serious impact on the spread of antibiotic resistance.

Cited by 7 publications

References 30 publications

Characterization of β-lactam resistance in K. pneumoniae associated with ready-to-eat processed meat in Egypt

Characterization of β-lactam resistance in K. pneumoniae associated with ready-to-eat processed meat in Egypt

A Comprehensive Review of Common Bacterial, Parasitic and Viral Zoonoses at the Human-Animal Interface in Egypt

Childhood diarrhoeal diseases in developing countries

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