Integrin-Linked Kinase Plays a Key Role in Coxsackievirus Replication

Lowenstein, Charles J.

doi:10.1161/01.res.0000239428.01434.e2

Cited by 3 publications

(2 citation statements)

References 10 publications

(13 reference statements)

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“…One such example is the observed increase of genes in the ILK signaling canonical pathway from 25 to 30. HCV induction of the ILK signaling canonical pathway (reported here for the first time) is likely to trigger actin rearrangement 33. Such cytoskeleton regulation is postulated to be important for HCV replication,34, 35 perhaps allowing viral movement to the tight junctions in a similar manner to Coxsackievirus 36, 37.…”

Section: Discussionmentioning

confidence: 69%

Transcriptome sequencing, microarray, and proteomic analyses reveal cellular and metabolic impact of hepatitis C virus infection in vitro

Woodhouse¹,

Narayan

Latham³

et al. 2010

Hepatology

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Hepatitis C virus (HCV) is a major cause of liver disease but the full impact of HCV infection on the hepatocyte is poorly understood. RNA sequencing (RNA-Seq) is a novel method to analyze the full transcriptional activity of a cell or tissue, thus allowing new insight into the impact of HCV infection. We conducted the first full-genome RNA-Seq analysis in a host cell to analyze infected and noninfected cells, and compared this to microarray and proteomic analyses. The combined power of the triple approach revealed that HCV infection affects a number of previously unreported canonical pathways and biological functions, including pregnane X receptor/retinoic acid receptor activation as a potential host antiviral response, and integrin-linked kinase signaling as an entry factor. This approach also identified several mechanisms implicated in HCV pathogenesis, including an increase in reactive oxygen species. HCV infection had a broad effect on cellular metabolism, leading to increases in cellular cholesterol and free fatty acid levels, associated with a profound and specific decrease in cellular glucose levels. Conclusion: RNA-Seq technology, especially when combined with established methods, demonstrated that HCV infection has potentially wide-ranging effects on cellular gene and protein expression. This in vitro study indicates a substantial metabolic impact of HCV infection and highlights new mechanisms of virus–host interaction which may be highly relevant to pathogenesis in vivo. (Hepatology 2010;52:443–453)

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Section: Discussionmentioning

confidence: 69%

Transcriptome sequencing, microarray, and proteomic analyses reveal cellular and metabolic impact of hepatitis C virus infection in vitro

Woodhouse¹,

Narayan

Latham³

et al. 2010

Hepatology

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“…This protein is also a unique adaptor that connects cell adhesion molecules to intracellular signaling pathway factors [ 10 ]. Although no research proving whether ILK affects the replication of NDV has been performed, cumulative evidence suggests that the inhibition of ILK expression prevents Akt activation that in turn suppresses coxsackievirus B3 (CVB3) replication [ 6 , 20 ]. Apoptosis is an innate immune response of cells to viral infection [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus

Deng

Yin

et al. 2014

J Vet Sci

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Characteristic clinical manifestations of Newcastle disease include leukopenia and immunosuppression. Peripheral blood mononuclear cells (PBMCs) are the main targets of Newcastle disease virus (NDV) infection. To survey changes in proteomic expression in chicken PBMCs following NDV infection, PBMC proteins from 30 chickens were separated using two-dimensional electrophoresis (2-DE) and subjected to mass spectrometry analysis. Quantitative intensity analysis showed that the expression of 78 proteins increased more than two-fold. Thirty-five proteins exhibited consistent changes in expression and 13 were identified as unique proteins by matrix assisted laser desorption ionization-time of flight mass spectrometer/mass spectrometer including three that were down-regulated and 10 that were up-regulated. These proteins were sorted into five groups based on function: macromolecular biosynthesis, cytoskeleton organization, metabolism, stress responses, and signal transduction. Furthermore, Western blot analysis confirmed the down-regulation of integrin-linked kinase expression and up-regulation of lamin A production. These data provide insight into the in vivo response of target cells to NDV infection at the molecular level. Additionally, results from this study have helped elucidate the molecular pathogenesis of NDV and may facilitate the development of new antiviral therapies as well as innovative diagnostic methods.

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Tyrosine kinase/phosphatase inhibitors decrease dengue virus production in HepG2 cells

Limjindaporn

Panaampon

Malakar

et al. 2017

Biochemical and Biophysical Research Communications

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Integrin-Linked Kinase Plays a Key Role in Coxsackievirus Replication

Cited by 3 publications

References 10 publications

Transcriptome sequencing, microarray, and proteomic analyses reveal cellular and metabolic impact of hepatitis C virus infection in vitro

Transcriptome sequencing, microarray, and proteomic analyses reveal cellular and metabolic impact of hepatitis C virus infection in vitro

Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus

Tyrosine kinase/phosphatase inhibitors decrease dengue virus production in HepG2 cells

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