Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus

Abstract

Characteristic clinical manifestations of Newcastle disease include leukopenia and immunosuppression. Peripheral blood mononuclear cells (PBMCs) are the main targets of Newcastle disease virus (NDV) infection. To survey changes in proteomic expression in chicken PBMCs following NDV infection, PBMC proteins from 30 chickens were separated using two-dimensional electrophoresis (2-DE) and subjected to mass spectrometry analysis. Quantitative intensity analysis showed that the expression of 78 proteins increased more than two-fold. Thirty-five proteins exhibited consistent changes in expression and 13 were identified as unique proteins by matrix assisted laser desorption ionization-time of flight mass spectrometer/mass spectrometer including three that were down-regulated and 10 that were up-regulated. These proteins were sorted into five groups based on function: macromolecular biosynthesis, cytoskeleton organization, metabolism, stress responses, and signal transduction. Furthermore, Western blot analysis confirmed the down-regulation of integrin-linked kinase expression and up-regulation of lamin A production. These data provide insight into the in vivo response of target cells to NDV infection at the molecular level. Additionally, results from this study have helped elucidate the molecular pathogenesis of NDV and may facilitate the development of new antiviral therapies as well as innovative diagnostic methods.

2-Deoxyglucose and Newcastle Disease Virus Synergize to Kill Breast Cancer Cells by Inhibition of Glycolysis Pathway Through Glyceraldehyde3-Phosphate Downregulation

“…Hence, based on the results of this study, we propose that NDV may further interfere with the glycolysis pathway by different mechanisms that amplify the glycolysis inhibition, leading to the starvation of cancer cells. Deng et al (2014) suggested that NDV may reduce the activity of the host's glycolytic pathway by decreasing phosphoglycerate kinase (PGK) expression in the NDV-infected PBMCs. PGK is a glycolytic enzyme that plays an important role in cellular energy production, and it forms part of the glycolytic pathway (Wang et al, 2010).…”

“…When synergized with chemotherapeutic agents, both NDV and 2-DG were found to increase significantly the anti-tumor activity in immunocompetent mice (Al-Shammari et al, 2011; Bénéteau et al, 2012). Moreover, both 2DG and NDV were shown to reduce the activity of the glycolytic pathway (Aft et al, 2002; Deng et al, 2014). Li et al (2015) found that the attenuated measles vaccine virus shifted the cellular metabolism to a high rate of glycolytic adaptation, which was efficiently targeted by dichloroacetate (DCA) (an inhibitor of glycolysis), leading to marked anti-tumor activity both in vitro and in vivo without any toxicity observed in normal cells.…”

Hexokinase inhibition using D-Mannoheptulose enhances oncolytic newcastle disease virus-mediated killing of breast cancer cells

“…NDV induced apoptosis via caspase-dependent and caspase-independent pathways [ 24 , 25 ]. Deng et al [ 26 ] found in a proteomic study that NDV downregulate phosphoglycerate kinase (PGK) expression in the NDV-infected chicken peripheral blood mononuclear cells (PBMCs), PGK is a glycolytic enzyme that participates in the glycolytic pathway, which suggests that NDV may restrict the infected cell glycolytic pathway. Its reported that interfering with cancer cells metabolism through glycolysis inhibition may enhance oncolytic virotherapy activity [ 27 ].…”

Global quantitative proteomic analysis profiles host protein expression in response to Sendai virus infection

“…Given that viruses rely on host proteins to replicate, one of the focuses of antiparamyxovirus research involves identifying host proteins involved in virus infection process. In recent years, to identify host proteins involved in the paramyxovirus infection process and to study paramyxovirus–host interactions systematically, several quantitative proteomic analyses have been performed on cells infected with different paramyxoviruses, including measles virus , Newcastle disease virus , and respiratory syncytial virus .…”