Integrin expression in oral hairy leukoplakia and normal tongue epithelium

Abstract

OBJECTIVE: To describe the expression of integrins in the epithelium of oral hairy leukoplakia (HL) and compare to that of normal lateral tongue epithelium. MATERIALS AND METHODS: Immunohistochemistry to identify integrins (α2, a α5, α6, αv, β1) was performed, using a standard biotin‐streptavidin‐peroxidase technique on five clinically and histologically confirmed frozen biopsy specimens of HL and five normal lateral tongue control tissues. RESULTS: Expression of integrins α2, α3, α6, αv, β1 was seen both in HL epithelium and in normal control tissue.α5 expression was not seen in HL or in control tissue epithelium.α2 and α3 were expressed mainly in the basal and suprabasal layers; α6 expression was most intense on the basal surface of the basal cells, αv was expressed in the basal and suprabasal layers with more expression seen in the higher differentiated cell layers than the other integrins.β1 expression was seen in the basal and suprabasal layers only. No apparent difference between HL and normal oral mucosa was noted in the staining pattern of the various integrins. CONCLUSION: Integrins α2, α3, α6, αv, β1 are expressed in HL and the expression pattern is not different from that of normal oral mucosa.α5 is not expressed in HL or in normal oral epithelium.

Lateral periodontal cyst: a clinicopathological study of 23 cases and an immunohistochemical analysis of its characteristic epithelial plaques in the lining

“…No histopathogenesis of the characteristic epithelial plaques in the LPC lining has been discussed in the literature, although their presence in LPC has been widely referred to (2)(3)(4)(5)(6)(7)(8)(9)(10)(11). In the present observation, we demonstrated for the first time that the uppermost cells of the epithelial plaque had some basal cell characteristics based on their immunohistochemical positivities for CK19 and integrin β1, which were usually found in the basal cells of the oral squamous epithelia (15)(16)(17)(18). This finding might be important for speculating how epithelial plaque is generated.…”

Nm23‐H1 promotes adhesion of CAL 27 cells in vitro

“…Integrins α1β1, α2β1, α3β1, α6β1, α9β1, and α6β4 are collagen‐binding integrins in healthy epithelia, of those only α1β1 and α2β1 bind collagen directly due to a presence of I‐domain in the alpha subunit. αvβ1, α5β1, αvβ5, and αvβ6 are oral epithelia, fibronectin‐binding integrins 27–29. When examining cell adhesion, we have found that nm23‐H1‐overexpressing cells tend to be different from the control cells only in the early adhesion process (90 min), while 24 h after seeding the examined differences are negligible.…”