Integrative Repair of Cartilage with Articular and Nonarticular Chondrocytes

Johnson, Timothy S.; Xu, Jiake; Zaporojan, Victor; Mesa, John; Weinand, Christian; Randolph, Mark A.; Bonassar, Lawrence J.; Winograd, Jonathan M.; Yaremchuk, Michael J.

doi:10.1089/1076327042500445

Cited by 21 publications

(27 citation statements)

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“…208 In another study, the potential of chondrocytes suspended in fibrin gels to integrate with native articular cartilage did not depend on the source of the chondrocytes. 210 However, many of these studies utilized a subcutaneous implantation model, which more closely resembles the auricular environment than the articular environment. More studies are required to determine the effectiveness of the chondrocyte sources in repairing articular cartilage defects.…”

Section: Cell Source and Seeding Densitymentioning

confidence: 99%

Hydrogels for the Repair of Articular Cartilage Defects

Spiller

Maher

Lowman

2011

Tissue Engineering Part B: Reviews

400

312

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Section: Cell Source and Seeding Densitymentioning

confidence: 99%

Hydrogels for the Repair of Articular Cartilage Defects

Spiller

Maher

Lowman

2011

Tissue Engineering Part B: Reviews

400

312

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“…The use of heterotopic chondrocytes as an additional cell source for ACT to improve articular cartilage repair is a promising novel approach (15)(16)(17)(18)(19)(20). To assess possible limitations of this strategy, a detailed characterization of heterotopic chondrocyte biology is necessary.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, Johnson et al (16) indicated that heterotopic chondrocytes adhere on hyaline cartilage slices. These facts altogether encourage the use of heterotopic chondrocytes for articular cartilage repair suggesting some plasticity and possibly transdifferentiation potential of heterotopic chondrocytes, which might be influenced and further guided by the unique local biochemical and biomechanical conditions in vivo.…”

Section: Discussionmentioning

confidence: 99%

“…After prolonged monolayer culture chondrocytes are irreversibly dedifferentiated and no longer suitable for ACT since they do not produce a hyaline-like ECM (9)(10)(11)(12). The use of heterotopic chondrocytes as an additional non-articular cell source might be a promising novel approach in articular cartilage repair and has currently started to attract more and more attention (13)(14)(15)(16)(17)(18)(19)(20)(21). Auricular cartilage contains a considerably higher number of cells and is much more easily accessible as well as being characterized by a lower donor site morbidity, compared with articular cartilage (18).…”

Section: Characterization Of Auricular Chondrocytes and Auricular/artmentioning

confidence: 99%

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Characterization of auricular chondrocytes and auricular/articular chondrocyte co-cultures in terms of an application in articular cartilage repair

Schulze-Tanzil¹

2010

Int J Mol Med

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Abstract. Cartilage injury remains a challenge in orthopedic surgery as articular cartilage only has a limited capacity for intrinsic healing. Autologous chondrocyte transplantation (ACT) is a suitable technique for cartilage repair, but requires articular cartilage biopsies for autologous chondrocyte expansion. The use of heterotopic chondrocytes derived from non-articular cartilage sources such as auricular chondrocytes may be a novel approach for ACT. The aim of the study is to evaluate whether co-cultured articular/auricular chondrocytes exhibit characteristics comparable to articular chondrocytes. Analysis of the proliferation rate, extracellular cartilage matrix (ECM) gene and protein expression (type II and I collagen, elastin, lubricin), ß1-integrins and the chondrogenic transcription factor sox9 in articular/auricular chondrocytes was performed using RTD-PCR, flow cytometry, immunofluorescence microscopy and Western blot analysis. Additionally, three-dimensional (3D) chondrocyte mono-and co-cultures were established. The proliferative activity and elastin gene expression were lower and that of type II collagen and lubricin was higher in articular compared with auricular chondrocytes. The species generally did not influence the chondrocyte characteristics, with the exception of type I collagen and sox9 expression, which was higher in porcine but not in human articular chondrocytes compared with both types of auricular chondrocytes. ß1-integrin gene expression did not differ significantly between the chondrocyte types. The type II collagen gene and protein expression was higher in articular chondrocyte monocultures and was slightly higher in co-cultures compared with monocultured auricular chondrocytes. Both chondrocyte types survived in co-culture. Despite their differing expression profiles, co-cultures revealed some adjustment in the ECM expression of both chondrocyte types.

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“…Fibrin coating on PLLA based scaffold have been found to improve scaffold adhesion properties thereby promoting early tissue regeneration [36]. Fibrin based hydrogel scaffolds have been widely used for tissue engineering applications of cartilage [37,38] and bone tissues [39,40].…”

Section: Fibrinmentioning

confidence: 99%

Naturally derived biomaterials for development of composite bone scaffold: A review

Barua

Deoghare

Deb

et al. 2018

IOP Conf. Ser.: Mater. Sci. Eng.

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E-mail: imon18enator@gmail.comAbstract. Over the last few decades, there has been an increasing demand of tissue engineered bone scaffolds as a substitute material for diseased or damaged bone segments. A variety of synthetic and natural biomaterials have been explored by researchers for development of composite scaffold. Naturally derived biomaterials have the advantages of immunomodulating, anti-toxic and biomimetic properties with the cellular environment in vivo, when compared to synthetic biomaterials. In this paper, different protein and polysaccharide based biomaterials used for developing composite bone scaffolds are reviewed. The properties of composite scaffolds developed from these biomaterials are highlighted. The study also includes different natural materials and biowastes which are used for deriving bioceramics for producing composite bone scaffolds. The overall study gives a brief idea on the importance of protein and polysaccharide based biomaterials and bioceramics for composite bone scaffold development and scope for future work in the field of naturally derived biomaterials.

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Integrative Repair of Cartilage with Articular and Nonarticular Chondrocytes

Cited by 21 publications

References 0 publications

Hydrogels for the Repair of Articular Cartilage Defects

Hydrogels for the Repair of Articular Cartilage Defects

Characterization of auricular chondrocytes and auricular/articular chondrocyte co-cultures in terms of an application in articular cartilage repair

Naturally derived biomaterials for development of composite bone scaffold: A review

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