Hydrogels for the Repair of Articular Cartilage Defects

Spiller, Kara L.; Maher, Suzanne A.; Lowman, Anthony M.

doi:10.1089/ten.teb.2011.0077

Cited by 391 publications

(318 citation statements)

References 249 publications

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“…Importantly, however, the aggregate modulus of the tissue engineered constructs was the same order of magnitude as that of native articular cartilage before deposition of ECM, and these properties were maintained throughout the culture period as the compressive properties are dominated by those of the 3D woven scaffold (50). In contrast, scaffolds composed of collagen, alginate, or fibrin-based hydrogels capable of encapsulating gene delivery vectors possess compressive properties below those of native articular cartilage (52)(53)(54). In this regard, the 3D woven structure provides several highly desirable properties as a scaffold for biomaterial-mediated gene delivery for in situ tissue engineering.…”

Section: Discussionmentioning

confidence: 99%

Scaffold-mediated lentiviral transduction for functional tissue engineering of cartilage

Brunger

Huynh

Guenther

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

112

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The ability to develop tissue constructs with matrix composition and biomechanical properties that promote rapid tissue repair or regeneration remains an enduring challenge in musculoskeletal engineering. Current approaches require extensive cell manipulation ex vivo, using exogenous growth factors to drive tissue-specific differentiation, matrix accumulation, and mechanical properties, thus limiting their potential clinical utility. The ability to induce and maintain differentiation of stem cells in situ could bypass these steps and enhance the success of engineering approaches for tissue regeneration. The goal of this study was to generate a self-contained bioactive scaffold capable of mediating stem cell differentiation and formation of a cartilaginous extracellular matrix (ECM) using a lentivirus-based method. We first showed that poly-L-lysine could immobilize lentivirus to poly(e-caprolactone) films and facilitate human mesenchymal stem cell (hMSC) transduction. We then demonstrated that scaffoldmediated gene delivery of transforming growth factor β3 (TGF-β3), using a 3D woven poly(e-caprolactone) scaffold, induced robust cartilaginous ECM formation by hMSCs. Chondrogenesis induced by scaffold-mediated gene delivery was as effective as traditional differentiation protocols involving medium supplementation with TGF-β3, as assessed by gene expression, biochemical, and biomechanical analyses. Using lentiviral vectors immobilized on a biomechanically functional scaffold, we have developed a system to achieve sustained transgene expression and ECM formation by hMSCs. This method opens new avenues in the development of bioactive implants that circumvent the need for ex vivo tissue generation by enabling the long-term goal of in situ tissue engineering.biomaterials | regenerative medicine | genetic engineering | gene therapy | chondrocyte

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Section: Discussionmentioning

confidence: 99%

Scaffold-mediated lentiviral transduction for functional tissue engineering of cartilage

Brunger

Huynh

Guenther

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

112

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“…[1][2][3][4] Articular cartilage allows for the relative movement of opposing joint surfaces under heavy loads, and cartilage defects are accompanied by persistent pain and functional limitations of the joint, but damaged articular cartilage displays a limited capacity for self-regeneration. 5,6 Current clinical practices for cartilage repair include microfracture, autologous chondrocyte implantation (ACI), and cartilage transplantation.…”

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confidence: 99%

Development and Characterization of Acellular Extracellular Matrix Scaffolds from Porcine Menisci for Use in Cartilage Tissue Engineering

Chen

Jhan

et al. 2015

Tissue Engineering Part C: Methods

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“…They are biomimetic per se since their high water content and diffusive transport properties very closely resemble those of the natural ECM 11 . The majority of hydrogels are also biocompatible, such as the ones based on natural polymers—agarose, alginate, chitosan, collagen, fibrin, gelatin, hyaluronic acid, dextran, silk, and matrigel 12 —as well as synthetic gels based on poly(ethylene glycol) (PEG), poly(vinyl alcohol) (PVA), poly(propylene fumarate), polyacrylic acid (PAA), and poly(hydroxyethyl methacrylate) (PHEMA) 13 .…”

Section: What Are Hydrogels?mentioning

confidence: 99%

Recent advances on gradient hydrogels in biomimetic cartilage tissue engineering

Gadjanski

2017

F1000Res

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Articular cartilage (AC) is a seemingly simple tissue that has only one type of constituting cell and no blood vessels and nerves. In the early days of tissue engineering, cartilage appeared to be an easy and promising target for reconstruction and this was especially motivating because of widespread AC pathologies such as osteoarthritis and frequent sports-induced injuries. However, AC has proven to be anything but simple. Recreating the varying properties of its zonal structure is a challenge that has not yet been fully answered. This caused the shift in tissue engineering strategies toward bioinspired or biomimetic approaches that attempt to mimic and simulate as much as possible the structure and function of the native tissues. Hydrogels, particularly gradient hydrogels, have shown great potential as components of the biomimetic engineering of the cartilaginous tissue.

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Hydrogels for the Repair of Articular Cartilage Defects

Cited by 391 publications

References 249 publications

Scaffold-mediated lentiviral transduction for functional tissue engineering of cartilage

Scaffold-mediated lentiviral transduction for functional tissue engineering of cartilage

Development and Characterization of Acellular Extracellular Matrix Scaffolds from Porcine Menisci for Use in Cartilage Tissue Engineering

Recent advances on gradient hydrogels in biomimetic cartilage tissue engineering

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