Integrative protocols for an in vitro generation of pancreatic progenitors from human dental pulp stem cells

Tailored generation of insulin producing cells from canine mesenchymal stem cells derived from bone marrow and adipose tissue

“…Our reports on the generation of IPCs from two sources of human dental tissue-derived MSCs, hDPSCs and human periodontal ligament stem cells (hPDLSCs), also showed the superior pancreatic differentiation potential of hDPSCs beyond hPDLSCs 21 , 40 . Recent publication from our team regarding bone tissue engineering also showed the distinct osteogenic differentiation potential by cBM-MSCs and canine dental pulp stem cells (cDPSCs) in vitro.…”

“…According to the analysis of the differentiated cells derived from the induction protocol, a set of characterization protocol was designed based on the characteristics of the IPCs, including colony morphology and size, pancreatic mRNA marker expression, mature pancreatic protein marker immunocytochemistry staining, and functional analysis based on glucose-stimulated C-peptide secretion (GSCS) analysis. Due to the IPC colony structure, enzymatic digestion to get a single cell population for flow cytometry analysis may cause the damage to the colony structure and particular cell components 21 , 40 .…”

“…It has been shown that the overexpression of PDX1 by lentiviral vector into mouse MSCs could enhance IPC generation by triggering the morphological change from adherent spindle fibroblast-like cells toward the ball-like cell colonies 38 , 83 . For cBM-MSCs, we found that a 3D culture condition was required to form the IPC colony, which was considered as the native pancreatic islet morphology 40 , 68 , 70 , 84 . Thus, cBM-MSC-derived IPCs were able to obtain from the integrating protocol of genetic and microenvironment manipulation.…”

Tailor-made generation of insulin-producing cells from canine mesenchymal stem cells derived from bone marrow and adipose tissue

“…In term of IPC formation in vitro, various protocols employing either microenvironmental manipulation or genetic manipulation have been reported 11,[32][33][34][35][36] . The strategies used in these studies usually relied on origin and pluripotency/multipotency of the cells [37][38][39][40][41] .…”

“…It has been shown that the overexpression of PDX1 by lentiviral vector into mouse MSCs could enhance IPC generation by triggering the morphological change from adherent spindle fibroblast-like cells toward the ball-like cell colonies 33,76 . For cBM-MSCs, we found that 3D culture condition was required to form the IPC colony which was considered as the native pancreatic islet morphology 35,63,65,77 . Thus, cBM-MSC-derived IPCs were able to obtain from the integrating protocol of genetic and microenvironmental manipulation.…”