Preconditioned human dental pulp stem cells with cerium and yttrium oxide nanoparticles effectively ameliorate diabetic hyperglycemia while combatting hypoxia

Ahmed, Hanaa H.; Aglan, Hadeer A.; Mahmoud, Nadia S.; Aly, Riham Mohamed

doi:10.1016/j.tice.2021.101661

Cited by 5 publications

(10 citation statements)

References 68 publications

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“…In fact, Pdx-1 expression was the highest of all the pancreatic genes studied both in DPSCs and PDLSCs. It was reported that Pdx-1 is an early marker of pancreatic development and beta cells synthesis [ 13 , 42 ]. Nicotinamide was also a common inducing factor in both protocols.…”

Section: Discussionmentioning

confidence: 99%

“…Although, several protocols for derivation of IPCs from various MSCs sources have been previously described, however only a few have reported the use of dental derived stem cells [ 9 – 13 ] Govindasamy et al, [ 11 ] was the first to report the ability of dental pulp stem cells (DPSCs) of deciduous teeth to trans-differentiate into pancreatic cell lineage and proposed that dental stem cells as a rather non-controversial source of stem cells which could be safely employed in autologous stem cell based therapies targeting diabetes. Shivakumar et al, [ 9 ] also recently assessed the differentiation potential of stem cells isolated from three sources of dental tissues into pancreatic beta cells.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of differentiation protocols of dental tissues stem cells to pancreatic β-cells

Aly

Aglan

Eldeen

et al. 2022

BMC Mol and Cell Biol

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Background Despite the recent progress in the differentiation strategies of stem cells into pancreatic beta cell lineage, current protocols are not optimized for different cell types. The purpose of this study is to investigate and compare the ability of stem cells derived from dental pulp (DPSCs) and periodontal ligament (PDLSCs) as two anatomically different dental tissues to differentiate into pancreatic beta cells while assessing the most suitable protocol for each cell type. Methods DPSCs & PDLSCs were isolated and characterized morphologically and phenotypically and then differentiated into pancreatic beta cells using two protocols. Differentiated cells were assessed by qRT-PCR for the expression of pancreatic related markers Foxa-2, Sox-17, PDX-1, Ngn-3, INS and Gcg. Functional assessment of differentiation was performed by quantification of Insulin release via ELISA. Results Protocol 2 implementing Geltrex significantly enhanced the expression levels of all tested genes both in DPSCs & PDLSCs. Both DPSCs & PDLSCs illustrated improved response to increased glucose concentration in comparison to undifferentiated cells. Moreover, DPSCs demonstrated an advanced potency towards pancreatic lineage differentiation over PDLSCs under both protocols. Conclusion In conclusion, the current study reports the promising potential of dental derived stem cells in differentiating into pancreatic lineage through selection of the right protocol.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimization of differentiation protocols of dental tissues stem cells to pancreatic β-cells

Aly

Aglan

Eldeen

et al. 2022

BMC Mol and Cell Biol

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“…The descriptive analysis of included animal studies is presented in Table 1. Seven studies were conducted in Japan [13,[46][47][48][49][50][51][52][53][54]; four in China [9,14,[55][56][57]; three in Egypt [58][59][60][61]; two in India [17,62]; and one in Brazil [63]. Mental state of rats.…”

Section: Characteristics Of Animal Studiesmentioning

confidence: 99%

“…Streptozotocin (STZ) was used to induce T1DM in 14 studies [9,13,17,46,48,[50][51][52]54,58,[60][61][62][63]; T2DM was induced using a high-fat diet in 2 studies [14,55], while in 1 study, T2DM was induced in rats which were given a high-fat diet and then administered with STZ [57]. Spraque Dawley (SD) rats were used in six studies [13,46,52,57,58,61]; Goto-Kakizaki (GK) rats were used in two [14,55]; Wistar rats in two [17,60]; Fischer 344 rats (F344-NJcl-rnu/rnu) in one study [54]; four studies used C57BL/6 mice [9,50,51,63]; and two used Bagg Albino (BALB/c) mice [48,62]. In 1 study, female mice were used [63], whereas 16 studies used male rats or mice.…”

Section: Animal Model Characteristicsmentioning

confidence: 99%

“…SHED were used in 6 studies [14,50,51,55,57,62], and human DPSCs (hDPSCs) were used in five [17,48,54,58,60]. In one study, MSCs from human gingiva (hGMSCs) were used [9], whereas in another, insulin-producing cells (IPCs) derived from human periodontal ligament stem cells (hPDLSCs) and hDPSCs were used [61].…”

Section: Dental Stem Cell Selection In Animal Studiesmentioning

confidence: 99%

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Dental Stem Cell-Based Therapy for Glycemic Control and the Scope of Clinical Translation: A Systematic Review and Meta-Analysis

Tonsekar,

Jiang

et al. 2024

IJTM

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Background: The tooth is a repository of stem cells, garnering interest in recent years for its therapeutic potential. The aim of this systematic review and meta-analysis was to test the hypothesis that dental stem cell administration can reduce blood glucose and ameliorate polyneuropathy in diabetes mellitus. The scope of clinical translation was also assessed. Methods: PubMed, Cochrane, Ovid, Web of Science, and Scopus databases were searched for animal studies that were published in or before July 2023. A search was conducted in OpenGrey for unpublished manuscripts. Subgroup analyses were performed to identify potential sources of heterogeneity among studies. The risk for publication bias was assessed by funnel plot, regression, and rank correlation tests. Internal validity, external validity, and translation potential were determined using the SYRCLE (Systematic Review Center for Laboratory Animal Experimentation) risk of bias tool and comparative analysis. Results: Out of 5031 initial records identified, 17 animal studies were included in the review. There was a significant decrease in blood glucose in diabetes-induced animals following DSC administration compared to that observed with saline or vehicle (SMD: −3.905; 95% CI: −5.633 to −2.177; p = 0.0004). The improvement in sensory nerve conduction velocity (SMD: 4.4952; 95% CI: 0.5959 to 8.3945; p = 0.035) and capillary-muscle ratio (SMD: 2.4027; 95% CI: 0.8923 to 3.9132; p = 0.0095) was significant. However, motor nerve conduction velocity (SMD: 3.1001; 95% CI: −1.4558 to 7.6559; p = 0.119) and intra-epidermal nerve fiber ratio (SMD: 1.8802; 95% CI: −0.4809 to 4.2413; p = 0.0915) did not increase significantly. Regression (p < 0.0001) and rank correlation (p = 0.0018) tests indicated the presence of funnel plot asymmetry. Due to disparate number of studies in subgroups, the analyses could not reliably explain the sources of heterogeneity. Interpretation: The direction of the data indicates that DSCs can provide good glycemic control in diabetic animals. However, methodological and reporting quality of preclinical studies, heterogeneity, risk of publication bias, and species differences may hamper translation to humans. Appropriate dose, mode of administration, and preparation must be ascertained for safe and effective use in humans. Longer-duration studies that reflect disease complexity and help predict treatment outcomes in clinical settings are warranted. This review is registered in PROSEPRO (number CRD42023423423).

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Combinatorial intervention with dental pulp stem cells and sulfasalazine in a rat model of ulcerative colitis

Aly,

Abohashem,

Ahmed

et al. 2024

Inflammopharmacol

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Background Ulcerative colitis is an inflammatory bowel disease (IBD) that involves inflammation of the colon lining and rectum. Although a definitive cure for IBD has not been identified, various therapeutic approaches have been proposed to mitigate the symptomatic presentation of this disease, primarily focusing on reducing inflammation. The aim of the present study was to evaluate the therapeutic potential of combining dental pulp stem cells (DPSCs) with sulfasalazine in an acetic acid-induced ulcerative colitis rat model and to assess the impact of this combination on the suppression of inflammatory cytokines and the regulation of oxidative stress in vivo. Methods Ulcerative colitis was induced in rats through transrectal administration of 3% acetic acid. The therapeutic effect of combining DPSCs and sulfasalazine on UC was evaluated by measuring the colonic weight/length ratio and edema markers; performing histopathological investigations of colon tissue; performing immunohistochemical staining for NF-κB-P65 and IL-1β; and evaluating oxidative stress and antioxidant indices via ELISA. Moreover, the proinflammatory markers NF-κB-P65, TNF-α and TLR-4 were assessed in colon tissue via ELISA. Furthermore, qRT‒PCR was used to estimate the expression levels of the TLR-4, NF-κB-P65, and MYD88 genes in colon tissue. Results The investigated macroscopic and microscopic signs of inflammation were markedly improved after the combined administration of sulfasalazine and DPSCs, where a noticeable improvement in histological structure, with an intact mucosal epithelium and mild inflammatory infiltration in the mucosa and submucosa, with slight hemorrhage. The administration of either DPSCs or sulfasalazine, either individually or in combination, significantly reduced ROS levels and significantly increased XOD activity. The immunohistochemical results demonstrated that the combined administration of DPSCs and sulfasalazine attenuated NFκB-p65 and IL-1β expression. Finally, the combined administration of DPSCs and sulfasalazine significantly downregulated MyD88, NF-κB and TLR4 gene expression. Conclusions Cotreatment with DPSCs and sulfasalazine had synergistic effects on ulcerative colitis, and these effects were relieved.

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Preconditioned human dental pulp stem cells with cerium and yttrium oxide nanoparticles effectively ameliorate diabetic hyperglycemia while combatting hypoxia

Cited by 5 publications

References 68 publications

Optimization of differentiation protocols of dental tissues stem cells to pancreatic β-cells

Optimization of differentiation protocols of dental tissues stem cells to pancreatic β-cells

Dental Stem Cell-Based Therapy for Glycemic Control and the Scope of Clinical Translation: A Systematic Review and Meta-Analysis

Combinatorial intervention with dental pulp stem cells and sulfasalazine in a rat model of ulcerative colitis

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