Integrative proteomics-metabolomics strategy reveals the mechanism of hepatotoxicity induced by Fructus Psoraleae

Duan, Jingyi; Dong, Wenying; Xie, Lijuan; Fan, Simiao; Xu, Yujuan; Li, Yubo

doi:10.1016/j.jprot.2020.103767

Cited by 28 publications

(15 citation statements)

References 61 publications

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“…It is also necessary to explore the protein composition and the distribution of BSFL for clarifying the high-value protein resource. Currently, proteomics is the dominant method for studying insect proteins, 32 while such research is still inadequate for BSFL.…”

Section: ■ Introductionmentioning

confidence: 99%

Proteomic Study Reveals Major Pathways Regulating the Development of Black Soldier Fly

Wan

et al. 2021

J. Proteome Res.

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Nowadays, biodegrading organic waste, as a solution to confront environmental challenges, has attracted wide attention. A dipteran insect, black soldier fly (BSF), exhibits outstanding capability to convert organic waste into proteins and lipid resources, and thus, much interest has been shown in it. However, information of fundamental biology of BSF is still limited besides its recycling efficiency. In this work, we present a complete proteomic database of BSF at all instars (before prepupa). We further formulated the pathways corresponding to BSF development and built a relationship with the current genetic database. To achieve this, we investigated the proteomics of BSF during different periods. We identified 5036 proteins, and among them, 3905 proteins were annotated in the protein function database. illustrated three pathways related to major physiological processes including the insulin signaling pathway for feeding and growth, fatty acid biosynthesis pathway for fatty acid using, and toll/immune deficiency pathway for immune behavior. The proteins in these three pathways were matched with a published genetic database, and this reference library could be used for future BSF genetic engineering. In conclusion, this work provided a comprehensive protein library of BSF and expands the basic knowledge of BSF for future research.

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Section: ■ Introductionmentioning

confidence: 99%

Proteomic Study Reveals Major Pathways Regulating the Development of Black Soldier Fly

Wan

et al. 2021

J. Proteome Res.

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“…As the adjuvant in HDT, Psoraleae Fructus has the function of tonifying kidney, strengthening yang , warming spleen and so on ( Xia et al, 2016 ). In recent years, more and more attention has been paid to hepatotoxicity induced by Psoraleae Fructus and its related preparations ( Cheung et al, 2009 , Duan et al, 2020 , Li et al, 2019a ). Ps and IP were demonstrated to be culprit, which individually caused liver injury at the dose of 40 and 80 mg/kg for rats by suppression of bile acid excretion in the liver and accumulation of toxin in the hepatocytes ( Wang et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Identification of Q-Markers from Hedan Tablet by employing “spider-web” mode and taking compounds’ hepatotoxicity into account

Wang

Ding

Feng

et al. 2022

Chinese Herbal Medicines

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“…In the past ten years, the research on the mechanism of reducing toxicity after pf processing has increased. However, studies mostly focus on chemical composition changes [ 21 , 22 ], and there is a lack of research on its metabolic differences in vivo. Therefore, we explored the potential mechanism of salt processing to reduce PF toxicity from the perspective of metabolism in vivo through animal serum metabolomics technology.…”

Section: Discussionmentioning

confidence: 99%

UPLC-MS/MS-Based Rat Serum Metabolomics Reveals the Detoxification Mechanism of Psoraleae Fructus during Salt Processing

Wang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Psoraleae Fructus (PF) is a botanical medicine widely used in Asian countries, of which salt products have higher safety and efficacy. However, the biological mechanism of the detoxification of salt-processing Psoraleae Fructus (SPF) has not yet been revealed. In this study, UPLC-MS/MS technology was used to explore the metabolic differences between SPF and PF in normal rats and reveal the mechanism of salt processing. The histopathological results of rat liver and kidney showed that the degree of liver and kidney injure in the SPF group was less than that in the PF group. The results of metabolomics showed that the detoxification mechanism of PF by salt processing might be related to glycerophospholipid metabolism, phenylalanine, tyrosine, and tryptophan biosynthesis, arginine and proline metabolism, phenylalanine metabolism, and linoleic acid metabolism. PF-induced inflammation could be reduced by regulating the expression of metabolites to achieve the purpose of salt processing and detoxification. It included reducing the production of metabolites such as 1-acyl-sn-glycero-3-phosphocholine, sn-glycero-3-phosphocholine, tyrosine, arginine, linoleic acid, arachidonic acid, and phenylacetylglycine/hippuric acid ratio and upregulating the expression of metabolites such as creatine.

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Integrative proteomics-metabolomics strategy reveals the mechanism of hepatotoxicity induced by Fructus Psoraleae

Cited by 28 publications

References 61 publications

Proteomic Study Reveals Major Pathways Regulating the Development of Black Soldier Fly

Proteomic Study Reveals Major Pathways Regulating the Development of Black Soldier Fly

Identification of Q-Markers from Hedan Tablet by employing “spider-web” mode and taking compounds’ hepatotoxicity into account

UPLC-MS/MS-Based Rat Serum Metabolomics Reveals the Detoxification Mechanism of Psoraleae Fructus during Salt Processing

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