Integrative plasma proteomic and microRNA analysis of Jersey cattle in response to high-altitude hypoxia

Kong, Zhiwei; Zhou, Changlin; Li, Bin; Jiao, Jinzhen; Chen, Liang; Ren, Ao; Jie, Hongdong; Tan, Zhiliang

doi:10.3168/jds.2018-15515

Cited by 20 publications

(20 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hypoxia increases fatty acid metabolism, and reduces fat synthesis and storage [6]. Previous researchers have explored how humans, pigs, dogs, and horses adapt to high-altitude hypoxia [7]. Additionally, the proteomics approach was applied to study the adaptive mechanism to hypoxia in human [8], mouse [9], and oriental river prawn [10].…”

Section: Introductionmentioning

confidence: 99%

Multi-Omics Analysis Reveals Up-Regulation of APR Signaling, LXR/RXR and FXR/RXR Activation Pathways in Holstein Dairy Cows Exposed to High-Altitude Hypoxia

Kong

Zhou

Chen

et al. 2019

Animals

Self Cite

View full text Add to dashboard Cite

Changes in the environment such as high-altitude hypoxia (HAH) high-altitude hypoxia can lead to adaptive changes in the blood system of mammals. However, there is limited information about the adaptation of Holstein dairy cows introduced to high-altitude areas. This study used 12 multiparous Holstein dairy cows (600 ± 55 kg, average three years old) exposed to HAH conditions in Nyingchi of Tibet (altitude 3000 m) and HAH-free conditions in Shenyang (altitude 50 m). The miRNA microarray analysis and iTRAQ proteomics approach (accepted as more suitable for accurate and comprehensive prediction of miRNA targets) were applied to explore the differences in the plasma proteomic and miRNA profiles in Holstein dairy cows. A total of 70 differential miRNAs (54 up-regulated, Fold change (FC) FC > 2, and 16 down-regulated, FC < 0.5) and 226 differential proteins (132 up-regulated, FC > 1.2, and 94 down-regulated, FC < 0.8) were found in the HAH-stressed group compared with the HAH-free group. Integrative analysis of proteomic and miRNA profiles demonstrated the biological processes associated with differential proteins were the immune response, complement activation, protein activation, and lipid transport. The integrative analysis of canonical pathways were most prominently associated with the APR signaling (z = 1.604), and LXR/RXR activation (z = 0.365), and FXR/RXR activation (z = 0.446) pathways. The current results indicated that Holstein dairy cows exposed to HAH could adapt to high-altitude hypoxia by up-regulating the APR, activating the LXR/RXR and FXE/RXR pathways.

show abstract

Section: Introductionmentioning

confidence: 99%

Multi-Omics Analysis Reveals Up-Regulation of APR Signaling, LXR/RXR and FXR/RXR Activation Pathways in Holstein Dairy Cows Exposed to High-Altitude Hypoxia

Kong

Zhou

Chen

et al. 2019

Animals

Self Cite

View full text Add to dashboard Cite

show abstract

“…These events are consistent with chronic stress affecting the physical conditions and increasing the vulnerability of animals to infection and disease. Several studies have investigated the adaptation of cattle managed on pasture in high-altitude regions (86,87). Kong et al investigated the miRNA expression profiles of two breeds of cattle, and found that the levels of circulating miR-155 and miR-17-5p were upregulated in Jersey cows (86), whereas miR-let7a-5p, miR-19a, and miR-181a were upregulated in Holstein cows (87).…”

Section: Environmental Stressmentioning

confidence: 99%

“…Several studies have investigated the adaptation of cattle managed on pasture in high-altitude regions (86,87). Kong et al investigated the miRNA expression profiles of two breeds of cattle, and found that the levels of circulating miR-155 and miR-17-5p were upregulated in Jersey cows (86), whereas miR-let7a-5p, miR-19a, and miR-181a were upregulated in Holstein cows (87). In both studies, hypoxia induced the upregulation of miRNAs involved in acute phase response (APR) signaling (86,87), which is known to promote homeostatic mechanisms in response to inflammatory stimuli (88).…”

Section: Environmental Stressmentioning

confidence: 99%

“…Kong et al investigated the miRNA expression profiles of two breeds of cattle, and found that the levels of circulating miR-155 and miR-17-5p were upregulated in Jersey cows (86), whereas miR-let7a-5p, miR-19a, and miR-181a were upregulated in Holstein cows (87). In both studies, hypoxia induced the upregulation of miRNAs involved in acute phase response (APR) signaling (86,87), which is known to promote homeostatic mechanisms in response to inflammatory stimuli (88). These miRNAs likely modulate inflammation-stimulating factors and the immune response, exerting an important role in the bovine resistance to high-altitude hypoxia, and represent potential candidate stressrelated biomarkers.…”

Section: Environmental Stressmentioning

confidence: 99%

See 1 more Smart Citation

MicroRNAs as Biomarkers for Animal Health and Welfare in Livestock

et al. 2020

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are small and highly conserved non-coding RNA molecules that orchestrate a wide range of biological processes through the post-transcriptional regulation of gene expression. An intriguing aspect in identifying these molecules as biomarkers is derived from their role in cell-to-cell communication, their active secretion from cells into the extracellular environment, their high stability in body fluids, and their ease of collection. All these features confer on miRNAs the potential to become a non-invasive tool to score animal welfare. There is growing interest in the importance of miRNAs as biomarkers for assessing the welfare of livestock during metabolic, environmental, and management stress, particularly in ruminants, pigs, and poultry. This review provides an overview of the current knowledge regarding the potential use of tissue and/or circulating miRNAs as biomarkers for the assessment of the health and welfare status in these livestock species.

show abstract

“…Guan et al [ 10 ] revealed that differentially expressed (DE) miRNAs in heart and lung between yak and cattle enriched hypoxia-related pathways, including the HIF-1 signalling, insulin signalling, PI3 K-Akt signalling, nucleotide excision repair, cell cycle, apoptosis and fatty acid metabolism. Kong et al [ 11 ] investigated changes in Jersey cattle in response to high-altitude hypoxia (HAH) compared with HAH-free condition. The results indicated that under HAH condition, Jersey cattle regulated inflammatory homeostasis by inhibiting the acute phase response, coagulation system, complement system and promoting liver X receptor/retinoid X receptor (LXR/RXR) activation.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis identified long non-coding RNAs involved in the adaption of yak to high-altitude environments

et al. 2020

View full text Add to dashboard Cite

The mechanisms underlying yak adaptation to high-altitude environments have been investigated using various methods, but no report has focused on long non-coding RNA (lncRNA). In the present study, lncRNAs were screened from the gluteus transcriptomes of yak and their transcriptional levels were compared with those in Sanjiang cattle, Holstein cattle and Tibetan cattle. The potential target genes of the differentially expressed lncRNAs between species/strains were predicted using cis and trans models. Based on cis -regulated target genes, no KEGG pathway was significantly enriched. Based on trans -regulated target genes, 11 KEGG pathways in relation to energy metabolism and three KEGG pathways associated with muscle contraction were significantly enriched. Compared with cattle strains, transcriptional levels of acyl-CoA dehydrogenase, acyl-CoA-binding protein, 3-hydroxyacyl-CoA dehydrogenase were relatively higher and those of glyceraldehyde 3-phosphate dehydrogenase, phosphoglycerate mutase 1, pyruvate kinase and lactate/malate dehydrogenase were relatively lower in yak, suggesting that yaks activated fatty acid oxidation but inhibited glucose oxidation and glycolysis. Besides, NADH dehydrogenase and ATP synthase showed lower transcriptional levels in yak than in cattle, which might protect muscle tissues from deterioration caused by reactive oxygen species (ROS). Compared with cattle strains, the higher transcriptional level of glyoxalase in yak might contribute to dicarbonyl stress resistance. Voltage-dependent calcium channel/calcium release channel showed a lower level in yak than in cattle strains, which could reduce the Ca 2+ influx and subsequently decrease the risk of hypertension. However, levels of EF-hand and myosin were higher in yak than in cattle strains, which might enhance the negative effects of reduced Ca 2+ on muscle contraction. Overall, the present study identified lncRNAs and proposed their potential regulatory functions in yak.

show abstract

Integrative plasma proteomic and microRNA analysis of Jersey cattle in response to high-altitude hypoxia

Cited by 20 publications

References 62 publications

Multi-Omics Analysis Reveals Up-Regulation of APR Signaling, LXR/RXR and FXR/RXR Activation Pathways in Holstein Dairy Cows Exposed to High-Altitude Hypoxia

Multi-Omics Analysis Reveals Up-Regulation of APR Signaling, LXR/RXR and FXR/RXR Activation Pathways in Holstein Dairy Cows Exposed to High-Altitude Hypoxia

MicroRNAs as Biomarkers for Animal Health and Welfare in Livestock

Transcriptome analysis identified long non-coding RNAs involved in the adaption of yak to high-altitude environments

Contact Info

Product

Resources

About