Integrative network analysis of rifampin-regulated miRNAs and their functions in human hepatocytes

Li, Jin; Wang, Ying; Wang, Lei; Liang, Hong; Feng, Weixing; Meng, Xianglian; Wang, Cong; Liu, Yunlong

doi:10.3233/bme-151502

Cited by 4 publications

(4 citation statements)

References 35 publications

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“…The patients enrolled in the present study were treated with same antibiotic regimen of doxycycline and rifampicin. In two previous studies, the potential effect of rifampicin on the expression levels of miRNA was demonstrated [ 38 , 39 ]. However, besides those two studies, the effects of antibiotics on miRNA expressions in other types of cells have not been examined.…”

Section: Discussionmentioning

confidence: 99%

The microRNA expression signature of CD4+ T cells in the transition of brucellosis into chronicity

et al. 2018

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Brucellosis is a serious infectious disease that continues to be a significant cause of morbidity worldwide and across all ages. Despite early diagnosis and treatment, 10–30% of patients develop chronic brucellosis. Although there have been recent advances in our knowledge of Brucella virulence factors and hosts’ immune response to the infection, there is a lack of clear data regarding how the infection bypasses the immune system and becomes chronic. The present study investigated immunological factors and their roles in the transition of brucellosis from an acute to a chronic infection in CD4+ T cells. CD4+ T cells sorted from peripheral blood samples of patients with acute or chronic brucellosis and healthy controls using flow cytometry as well as more than 2000 miRNAs were screened using the GeneSpring GX (Agilent) 13.0 miRNA microarray software and were validated using reverse transcription polymerase chain reaction (RT-qPCR). Compared to acute cases, the expression levels of 28 miRNAs were significantly altered in chronic cases. Apart from one miRNA (miR-4649-3p), 27 miRNAs were not expressed in the acute cases (p <0.05, fold change> 2). According to KEGG pathway analysis, these miRNAs are involved in the regulation of target genes that were previously involved in the MAPK signalling pathway, regulation of the actin cytoskeleton, endocytosis, and protein processing in the endoplasmic reticulum. This indicates the potential role of these miRNAs in the development of chronic brucellosis. We suggest that these miRNAs can be used as markers to determine the transition of the disease into chronicity. This is the first study of miRNA expression that analyses human CD4+ T cells to clarify the mechanism of chronicity in brucellosis.

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Section: Discussionmentioning

confidence: 99%

The microRNA expression signature of CD4+ T cells in the transition of brucellosis into chronicity

et al. 2018

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“…In this study, all patients received the same antibiotic regimen including doxycycline and rifampicin. In two studies, it was demonstrated that rifampicin may alter miRNA expression in human hepatocyte cultures [ 38 , 39 ]. Apart from those studies, there are no studies available that display the role of antibiotics with regard to changes in miRNA expression in other cells including PBMCs in vitro or in vivo .…”

Section: Discussionmentioning

confidence: 99%

Altered Expressions of miR-1238-3p, miR-494, miR-6069, and miR-139-3p in the Formation of Chronic Brucellosis

Budak

Bal

Tezcan

et al. 2016

Journal of Immunology Research

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Brucellosis is a zoonotic disease that is still endemic in developing countries. Despite early diagnosis and treatment of patients, chronic infections are seen in 10–30% of patients. In this study, we aimed to investigate the immunological factors that play roles in the transition of brucellosis from acute infection into chronic infection. Here, more than 2000 miRNAs were screened in peripheral blood mononuclear cells (PBMCs) of patients with acute or chronic brucellosis and healthy controls by using miRNA array, and the results of the miRNA array were validated through qRT-PCR. Findings were evaluated using GeneSpring GX (Agilent) 13.0 software and KEGG pathway analysis. Four miRNAs were expressed in the chronic group but were not expressed in acute and control groups. Among these miRNAs, the expression level of miR-1238-3p was increased while miR-494, miR-6069, and miR-139-3p were decreased (p < 0.05, fold change > 2). These miRNAs have the potential to be markers for chronic cases. The differentially expressed miRNAs and their predicted target genes involved in endocytosis, regulation of actin cytoskeleton, MAPK signaling pathway, and cytokine-cytokine receptor interaction and its chemokine signaling pathway indicate their potential roles in chronic brucellosis and its progression. It is the first study of miRNA expression analysis of human PBMC to clarify the mechanism of inveteracy in brucellosis.

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“…In two studies, it was demonstrated that rifampicin may alter miRNA expression in human hepatocyte cultures [44, 45]. Apart from those studies, there are no studies available that display the role of antibiotics with regards to changes in miRNA expression in other cells in vitro or in vivo .…”

Section: Discussionmentioning

confidence: 99%

MicroRNA Expression Patterns of CD8+ T Cells in Acute and Chronic Brucellosis

et al. 2016

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Although our knowledge about Brucella virulence factors and the host response increase rapidly, the mechanisms of immune evasion by the pathogen and causes of chronic disease are still unknown. Here, we aimed to investigate the immunological factors which belong to CD8+ T cells and their roles in the transition of brucellosis from acute to chronic infection. Using miRNA microarray, more than 2000 miRNAs were screened in CD8+ T cells of patients with acute or chronic brucellosis and healthy controls that were sorted from peripheral blood with flow cytometry and validated through qRT-PCR. Findings were evaluated using GeneSpring GX (Agilent) 13.0 software and KEGG pathway analysis. Expression of two miRNAs were determined to display a significant fold change in chronic group when compared with acute or control groups. Both miRNAs (miR-126-5p and miR-4753-3p) were decreased (p <0.05 or fold change > 2). These miRNAs have the potential to be the regulators of CD8+ T cell-related marker genes for chronic brucellosis infections. The differentially expressed miRNAs and their predicted target genes are involved in MAPK signaling pathway, cytokine-cytokine receptor interactions, endocytosis, regulation of actin cytoskeleton, and focal adhesion indicating their potential roles in chronic brucellosis and its progression. It is the first study of miRNA expression analysis of human CD8+ T cells to clarify the mechanism of inveteracy in brucellosis.

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Integrative network analysis of rifampin-regulated miRNAs and their functions in human hepatocytes

Cited by 4 publications

References 35 publications

The microRNA expression signature of CD4+ T cells in the transition of brucellosis into chronicity

The microRNA expression signature of CD4+ T cells in the transition of brucellosis into chronicity

Altered Expressions of miR-1238-3p, miR-494, miR-6069, and miR-139-3p in the Formation of Chronic Brucellosis

MicroRNA Expression Patterns of CD8+ T Cells in Acute and Chronic Brucellosis

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