Altered Expressions of miR-1238-3p, miR-494, miR-6069, and miR-139-3p in the Formation of Chronic Brucellosis

Budak, Ferah; Bal, Salih Haldun; Tezcan, Gülçin; Akalın, Halis; Göral, Güher; Oral, Haluk Barbaros

doi:10.1155/2016/4591468

Cited by 22 publications

(30 citation statements)

References 85 publications

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“…Furthermore, we reviewed the literature in order to identify other possible roles or mechanisms in which miR-1226 participates in regulating bone metabolism. Interestingly, we found out that miR-1226 was one of the miRNAs able to differentiate acute from chronic disease cases of Brucellosis, being this miRNA specifically downregulated in chronic cases ( 28 ). In brucellosis disease, a serious loss of bone occurs ( 28 ).…”

Section: Discussionmentioning

confidence: 96%

miR-1226 detection in GCF as potential biomarker of chronic periodontitis: A pilot study

Micó-Martínez¹,

García-Giménez²,

Seco-Cervera³

et al. 2018

Med Oral

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BackgroundThe study and identification of new biomarkers for periodontal disease, such as microRNAs (miRNAs), may give us more information about the location and severity of the disease and will serve as a basis for treatment planning and disease-monitoring. miRNAs are a group of small RNAs which are involved in gene regulation by binding to their messenger RNA target (mRNA). In this pilot study, the procedure for purifying miRNAs from gingival crevicular fluid (GCF) was, for the first time, described. In addition, the concentration of miRNAs in GCF was analyzed and compared between patients with moderate or severe chronic periodontitis (CP) and healthy controls.Material and MethodsGCF samples were collected from single-rooted teeth of patients with moderate or severe CP (n=9) and of healthy individuals (n=9). miRNAs were isolated from GCF using miRNeasy Serum/Plasma kit (Qiagen, CA. USA). Reverse transcription polymerase chain reaction (qRT-PCR) was used to determine the expression of a series of miRNAs candidates that are related to bone metabolism. The significance of differences in miRNA levels between both groups was determined using Mann-Whitney U test.ResultsThe results from this pilot study indicate that miRNAs can be isolated from GCF. Six different miRNAs were analyzed (miR-671, miR-122, miR-1306, miR-27a, miR-223, miR-1226), but only miR-1226 showed statically significant differences between the CP group and healthy controls (p<0.05). This miRNA was downregulated in patients with CP.ConclusionsWithin the limitations of the present study, it may be concluded that miR-1226 can be a promising biomarker for periodontal disease, adding relevant information to common clinical parameters used for diagnosis and prognosis of periodontitis. Key words:Small interfering RNA, biomarkers, periodontal diseases, reverse transcriptase polymerase chain reaction.

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Section: Discussionmentioning

confidence: 96%

miR-1226 detection in GCF as potential biomarker of chronic periodontitis: A pilot study

Micó-Martínez¹,

García-Giménez²,

Seco-Cervera³

et al. 2018

Med Oral

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“…Recent reports have provided evidence that miRNA plays an important role in bacterial infections, including Brucella infection. [8][9][10][11][12][13] As an important innate immune molecule, CD14 is involved in the initiation of the inflammatory response to sepsis. However, there are few reports about the relationship between CD14 and the miRNA expression profile of host cells.…”

Section: Discussionmentioning

confidence: 99%

CD14 gene silencing alters the microRNA expression profile of RAW264.7 cells stimulated by Brucella melitensis infection

Hui

Hao

et al. 2017

Innate Immun

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Innate recognition of Brucella spp. is a key step in the activation of inflammation. CD14 binds PAMPs and is involved in LPS-induced pro-inﬂammatory cytokine release. Previously we showed that knock down of CD14 in RAW264.7 macrophages disrupted Brucella-host interactions. However, its effect on the macrophage microRNA (miRNA) expression profile, especially after stimulation by Brucella infection, is still unclear. To identify miRNAs involved in the macrophage response to Brucella infection, we performed miRNA expression profiling of CD14 knock-down RAW264.7 (224.3) macrophages infected with Brucella melitensis, and demonstrated, for the first time, that CD14 knock down significantly up-regulated the expression of mmu-miR-199a-3p and mmu-miR-183-5p in these conditions. These miRNAs have a well-characterized association with the target genes involved in immune response, inflammatory response, innate immune response, apoptosis processes, anti-apoptosis, cytokine production and cytokine-mediated signaling pathways. Among the 104 inflammation-related candidate target genes of mmu-miR-199a-3p and mmu-miR-183-5p in the 224.3 B. melitensis group cells, the expression of the Cbl-b, a potential target of mmu-miR-199a-3p, was confirmed to be down-regulated using qRT-PCR and Western blot analysis. Our findings suggest that CD14 functions in the Brucella-host interaction may be through altered miRNA expression, and regulation of Cbl-b proteins.

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“…Most of these studies are focused on circulating miRNAs in acute and chronic forms of Hepatitis or acute and chronic phases of Hepatitis C [40–42]. In our recent study, we have shown that reduced expression of miR-139-3p, miR-6069 and miR-494 and induced expression of miR-1238-3p were significantly associated with chronic brucellosis [43]. …”

Section: Discussionmentioning

confidence: 99%

“…In particular, a total of 25 target genes participate in regulating apoptosis and autophagy, indicating that these miRNAs may play important regulatory roles in the Brucella -host interactions [46]. In our previous study, more than 2000 miRNAs were screened in peripheral blood mononuclear cells of patients with acute or chronic brucellosis and we determined that while the expression level of miR-1238-3p was increased, miR-494, miR-6069 and miR-139-3p were decreased in the chronic group in comparison to the acute infection group [43]. In this current study, the regulatory role of 2000 miRNAs were evaluated in human CD8+ T cells.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA Expression Patterns of CD8+ T Cells in Acute and Chronic Brucellosis

et al. 2016

Self Cite

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Although our knowledge about Brucella virulence factors and the host response increase rapidly, the mechanisms of immune evasion by the pathogen and causes of chronic disease are still unknown. Here, we aimed to investigate the immunological factors which belong to CD8+ T cells and their roles in the transition of brucellosis from acute to chronic infection. Using miRNA microarray, more than 2000 miRNAs were screened in CD8+ T cells of patients with acute or chronic brucellosis and healthy controls that were sorted from peripheral blood with flow cytometry and validated through qRT-PCR. Findings were evaluated using GeneSpring GX (Agilent) 13.0 software and KEGG pathway analysis. Expression of two miRNAs were determined to display a significant fold change in chronic group when compared with acute or control groups. Both miRNAs (miR-126-5p and miR-4753-3p) were decreased (p <0.05 or fold change > 2). These miRNAs have the potential to be the regulators of CD8+ T cell-related marker genes for chronic brucellosis infections. The differentially expressed miRNAs and their predicted target genes are involved in MAPK signaling pathway, cytokine-cytokine receptor interactions, endocytosis, regulation of actin cytoskeleton, and focal adhesion indicating their potential roles in chronic brucellosis and its progression. It is the first study of miRNA expression analysis of human CD8+ T cells to clarify the mechanism of inveteracy in brucellosis.

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Altered Expressions of miR-1238-3p, miR-494, miR-6069, and miR-139-3p in the Formation of Chronic Brucellosis

Cited by 22 publications

References 85 publications

miR-1226 detection in GCF as potential biomarker of chronic periodontitis: A pilot study

miR-1226 detection in GCF as potential biomarker of chronic periodontitis: A pilot study

CD14 gene silencing alters the microRNA expression profile of RAW264.7 cells stimulated by Brucella melitensis infection

MicroRNA Expression Patterns of CD8+ T Cells in Acute and Chronic Brucellosis

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