Integrative genome-wide analysis reveals the role of WIP proteins in inhibition of growth and development

Roldán, Maria Victoria Gómez; Izhaq, Farhaj; Verdenaud, Marion; Eleblu, John Saviour Yaw; Haraghi, Aimen; Sommard, Vivien; Chambrier, Pierre; Latrasse, David; Jégu, Teddy; Benhamed, Moussa; Szécsi, Judit; Bendahmane, Mohammed; Boualem, Adnane

doi:10.1038/s42003-020-0969-2

Cited by 16 publications

(9 citation statements)

References 43 publications

(61 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…WIP1-binding sites were mostly identified in promoter and intergenic regions (data S1); a consensus motif (Fig. 3A) computed from these sequences corresponds to the binding site, as previously characterized by Chromatin Immunoprecipitation sequencing (ChIP-seq) and ampDAPseq for three Arabidopsis WIP proteins ( 16 , 17 ). In particular, we found that the CmCRC promoter harbored the consensus CmWIP1 binding motif and was bound by CmWIP1 but not by its zinc-finger variants G242R and S306F (Fig.…”

Section: Resultssupporting

confidence: 65%

See 1 more Smart Citation

The control of carpel determinacy pathway leads to sex determination in cucurbits

Zhang

Tan

Chung

et al. 2022

Science

Self Cite

View full text Add to dashboard Cite

Male and female unisexual flowers evolved from hermaphroditic ancestors, and control of flower sex is useful for plant breeding. We isolated a female-to-male sex transition mutant in melon and identified the causal gene as the carpel identity gene CRABS CLAW (CRC) . We show that the master regulator of sex determination in cucurbits, the transcription factor WIP1 whose expression orchestrates male flower development, recruits the corepressor TOPLESS to the CRC promoter to suppress its expression through histone deacetylation. Impairing TOPLESS-WIP1 physical interaction leads to CRC expression, carpel determination, and consequently the expression of the stamina inhibitor, the aminocyclopropane-1-carboxylic acid synthase 7 ( CmACS7 ), leading to female flower development. Our findings suggest that sex genes evolved to interfere with flower meristematic function, leading to unisexual flower development.

show abstract

Section: Resultssupporting

confidence: 65%

“…In flowering plants, WIPs are implicated in transmitting tracts, primary root formation, and sex determination ( 22 – 25 ). Based on their sequence homology and complementation experiments, it has been suggested that WIP genes might show conserved functions across species ( 16 ). Our work reveals the mode of action of WIP proteins.…”

Section: Discussionmentioning

confidence: 99%

The control of carpel determinacy pathway leads to sex determination in cucurbits

Zhang

Tan

Chung

et al. 2022

Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…Therefore, as the overexpression of all Arabidopsis WIP s has a similar effect on organ and cell size, cell growth modulation might be a common WIP function, at least for the six Arabidopsis WIP s and Gerbera GbWIP2 . The same negative effect on cell growth was also reported recently by [ 13 ] for the Arabidopsis WIP members WIP1 and WIP2 . In global expression analyses of overexpression lines, the authors found gene categories related to stress and cell growth repression and they observed a reduction in plant size in these lines [ 13 ].…”

Section: Discussionsupporting

confidence: 88%

“…The same negative effect on cell growth was also reported recently by [ 13 ] for the Arabidopsis WIP members WIP1 and WIP2 . In global expression analyses of overexpression lines, the authors found gene categories related to stress and cell growth repression and they observed a reduction in plant size in these lines [ 13 ].…”

Section: Discussionsupporting

confidence: 88%

Expression and Functional Analyses of the WIP Gene Family in Arabidopsis

Díaz-Ramírez

Diaz-Garcia

Magdaleno-Garcia

et al. 2022

Plants

View full text Add to dashboard Cite

The WIP family of transcription factors comprises the A1d subgroup of C2H2 zinc finger proteins. This family has six members in Arabidopsis thaliana and most of the known functions have been described by analyzing single knockout mutants. However, it has been shown that WIP2 and its closest paralogs WIP4 and WIP5 have a redundant and essential function in root meristems. It is likely that these and other WIP genes perform more, still unknown, functions. To obtain hints about these other functions, the expression of the six WIP genes was explored. Moreover, phenotypic ana-lyses of overexpressors and wip mutants revealed functions in modulating organ and cell size, stomatal density, and vasculature development.

show abstract

“…STOP1 (At1g34370) protects plants against acidic soils [ 32 ]. WIP-type AtC2H2-ZFPs, including WIP1 (AT1G34790) and WIP2 (AT3G57670), regulate endothelium differentiation, pollen tube development, and leaf vasculature growth in plants [ 33 , 34 ]. Apart from that, DAZ1 (AT2G17180) and DAZ2 (AT4G35280), which were in clade C-III, are involved in pollen fertility.…”

Section: Resultsmentioning

confidence: 99%

Genome-Wide Analysis of C2H2 Zinc Finger Gene Family and Its Response to Cold and Drought Stress in Sorghum [Sorghum bicolor (L.) Moench]

Cui

Chen

Liu

et al. 2022

IJMS

View full text Add to dashboard Cite

C2H2 zinc finger protein (C2H2-ZFP) is one of the most important transcription factor families in higher plants. In this study, a total of 145 C2H2-ZFPs was identified in Sorghum bicolor and randomly distributed on 10 chromosomes. Based on the phylogenetic tree, these zinc finger gene family members were divided into 11 clades, and the gene structure and motif composition of SbC2H2-ZFPs in the same clade were similar. SbC2H2-ZFP members located in the same clade contained similar intron/exon and motif patterns. Thirty-three tandem duplicated SbC2H2-ZFPs and 24 pairs of segmental duplicated genes were identified. Moreover, synteny analysis showed that sorghum had more collinear regions with monocotyledonous plants such as maize and rice than did dicotyledons such as soybean and Arabidopsis. Furthermore, we used quantitative RT-PCR (qRT-PCR) to analyze the expression of C2H2-ZFPs in different organs and demonstrated that the genes responded to cold and drought. For example, Sobic.008G088842 might be activated by cold but is inhibited in drought in the stems and leaves. This work not only revealed an important expanded C2H2-ZFP gene family in Sorghum bicolor but also provides a research basis for determining the role of C2H2-ZFPs in sorghum development and abiotic stress resistance.

show abstract

Integrative genome-wide analysis reveals the role of WIP proteins in inhibition of growth and development

Cited by 16 publications

References 43 publications

The control of carpel determinacy pathway leads to sex determination in cucurbits

The control of carpel determinacy pathway leads to sex determination in cucurbits

Expression and Functional Analyses of the WIP Gene Family in Arabidopsis

Genome-Wide Analysis of C2H2 Zinc Finger Gene Family and Its Response to Cold and Drought Stress in Sorghum [Sorghum bicolor (L.) Moench]

Contact Info

Product

Resources

About