<b>The control of carpel determinacy pathway leads to sex determination</b>
            <b>in cucurbits</b>

Zhang, Siqi; Tan, Feng-Quan; Chung, Ching‐Hui; Slavković, Filip; Devani, Ravi Suresh; Troadec, Christelle; Marcel, Fabien; Morin, Halima; Camps, Céline; Roldán, Maria Victoria Gómez; Benhamed, Moussa; Dogimont, Catherine; Boualem, Adnane; Bendahmane, Abdelhafid

doi:10.1126/science.add4250

Cited by 34 publications

(22 citation statements)

References 49 publications

(41 reference statements)

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“…6A ). Recently, a new study was published that described CRABS CLAW ( CRC ), a transcription factor of the YABBY gene family controlling carpel and nectary development, in addition to flower meristem termination, as a new player in the sex determination model in melon ( Zhang et al , 2022 ). Interestingly, in our RNA-Seq data this gene was overexpressed during the GI-H episode.…”

Section: Discussionmentioning

confidence: 99%

Developmental stages and episode-specific regulatory genes in andromonoecious melon flower development

Pechar,

Sánchez-Pina,

Coronado-Parra

et al. 2023

Annals of Botany

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Background and Aims Given the lack of specific studies on floral development in melon (Cucumis melo L.), we carried out an extensive study involving morphological and transcriptomic analyses to characterize floral development in this species. Methods Using an andromonoecious line, we analysed the development of floral buds in male and hermaphrodite flowers with both light microscopy and scanning electron microscopy. Based on flower lengths, we established a correlation between the developmental stages and four main episodes of floral development and conducted an extensive RNA sequencing analysis of these episodes. Key Results We identified 12 stages of floral development, from the appearance of the floral meristems to anthesis. The main structural differences between male and hermaphrodite flowers appeared between stages 6 and 7; later stages of development leading to the formation of organs and structures in both types of flowers were also described. We analysed the gene expression patterns of the four episodes in flower development to find the genes that were specific to each given episode. Among others, we identified genes that defined the passage from one episode to the next according to the ABCDE model of floral development. Conclusions This work combines a detailed morphological analysis and a comprehensive transcriptomic study to enable characterization of the structural and molecular mechanisms that determine the floral development of an andromonoecious genotype in melon. Taken together, our results provide a first insight into gene regulation networks in melon floral development that are crucial for flowering and pollen formation, highlighting potential targets for genetic manipulation to improve crop yield of melon in the future.

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Section: Discussionmentioning

confidence: 99%

Developmental stages and episode-specific regulatory genes in andromonoecious melon flower development

Pechar,

Sánchez-Pina,

Coronado-Parra

et al. 2023

Annals of Botany

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“…However, ongoing Y‐degeneration [ 48,49 ] may lead to eventual GSFY loss and evolution of X/A‐ratio system controlled by the number of X‐linked WUS genes. Interestingly, WUS is also involved in the sex expression pathway in kiwifruits, [ 16 ] and Cucumis , [ 50 ] implying that this mechanism possibly plays a role in gynoecium suppression of many plant groups with independently evolved dioecy and sex chromosomes. Further analyses of sub‐functionalization of GSFY or SlWUS1 and the effect of their copy numbers will allow one to test whether WUS / CLV3 ratio control the gynoecium suppression.…”

Section: Does the Wus/clv3 Balance Represent A Steppingstone In Evolu...mentioning

confidence: 99%

Evolution of sex‐determination in dioecious plants: From active Y to X/A balance?

Kazama,

Kobayashi,

Filatov

2023

BioEssays

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Sex chromosomes in plants have been known for a century, but only recently have we begun to understand the mechanisms behind sex determination in dioecious plants. Here, we discuss evolution of sex determination, focusing on Silene latifolia, where evolution of separate sexes is consistent with the classic “two mutations” model—a loss of function male sterility mutation and a gain of function gynoecium suppression mutation, which turned an ancestral hermaphroditic population into separate males and females. Interestingly, the gynoecium suppression function in S. latifolia evolved via loss of function in at least two sex‐linked genes and works via gene dosage balance between sex‐linked, and autosomal genes. This system resembles X/A‐ratio‐based sex determination systems in Drosophila and Rumex, and could represent a steppingstone in the evolution of X/A‐ratio‐based sex determination from an active Y system.

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“…8 of a hermaphroditic melon germplasm (Wang et al 2022), and a novel sex determination gene, CmCRC , has been identified on Chr. 10 (Zhang et al, 2022). Thus, the study of the genetic mechanism of melon sex determination has much potential, and novel genes may still be discovered in melon.…”

Section: Introductionmentioning

confidence: 99%

Identification of novel sex determination loci in Japanese weedy melon

Nashiki

Matsuo

Takano

et al. 2023

Preprint

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Sex expression contributes to fruit quality and yield in the Cucurbitaceae. In melon, orchestrated regulation by sex determination genes explains the mechanism of sex expression, resulting in a great variety of sexual morphologies. In this study, we examined the Japanese weedy melon UT1, which does not follow the reported model of sex expression. We conducted QTL analysis using F2 plants for flower sex on the main stem and the lateral branch and mapped a 'femaleness' locus on Chr. 3 (Fem3.1) and a 'type of flower femaleness' (female or bisexual) locus on Chr. 8 (tff8.1). Fem3.1 included the known sex determination gene <mACS11>. Sequence comparison of CmACS11 between parental lines revealed three nonsynonymous SNPs. A CAPS marker developed from one of the SNPs was closely linked to femaleness in two F2 populations with different genetic backgrounds. The femaleness allele was dominant in F1 lines from crosses between UT1 and diverse cultivars and breeding lines. This study suggests that the identity of tff8.1 is CmCPR5, a recently reported bisexual flower control gene. We found that the Japanese weedy melon UT1 does not follow the conventional sex expression model because of the interaction of the loci Fem3.1 and tff8.1 with the previously reported sex determination genes. The results of this study provide new insights into the molecular mechanisms of sex determination in melons and considerations for the application of femaleness in melon breeding.

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The control of carpel determinacy pathway leads to sex determination in cucurbits

Cited by 34 publications

References 49 publications

Developmental stages and episode-specific regulatory genes in andromonoecious melon flower development

Developmental stages and episode-specific regulatory genes in andromonoecious melon flower development

Evolution of sex‐determination in dioecious plants: From active Y to X/A balance?

Identification of novel sex determination loci in Japanese weedy melon

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