Integrative analysis of the metabolome and transcriptome reveals the molecular mechanism of chlorogenic acid synthesis in peach fruit

Su, Zhiyuan; Jia, Haiqiang; Sun, Meng; Cai, Zeyu; Shen, Zhijun; Zhao, Bintao; Li, Jiyao; Ma, Ruijuan; Yu, Mingliang; Yan, Juan

doi:10.3389/fnut.2022.961626

Cited by 11 publications

(5 citation statements)

References 45 publications

(61 reference statements)

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“…As one of the main phenolic compounds, CGA is abundant in various fruits, including apples, pears, peaches, and strawberries [1,4,6,25]. In this study, we found that the CGA content in both apple cultivars decreased gradually during the development, which was consistent with the previous report on apple fruits [1].…”

Section: Discussionsupporting

confidence: 92%

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Transcriptome Analysis Identifies Genes Associated with Chlorogenic Acid Biosynthesis during Apple Fruit Development

et al. 2023

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As a predominant phenolic compound in apple fruits, chlorogenic acid (CGA) benefits human health due to its various antioxidant properties. However, little has been known regarding the molecular mechanism underlying the CGA accumulation in apple fruits. In this study, we measured the CGA content and relative enzymes’ activities during fruit development in two different flesh-colored cultivars ‘Huashuo’ and ‘Red Love’. The CGA content in both cultivars decreased sharply from 30 days after full bloom (DAFB) to 60 DAFB. Notably, the CGA content in fruit flesh was relatively higher than that in the peel. Further, the activities of C3H and HCT enzymes downstream of the CGA biosynthesis showed the similar changing trend as CGA content. Based on the transcriptome data of ‘Huashuo’ fruit at 30 DAFB and 60 DAFB, 23 differentially expressed CGA synthesis-related genes were screened. Gene expression analyses further showed that MdHCT1/2/4/5/6 and MdC3H1/2/3 were positively correlated to the variation of CGA content in two cultivars. These findings establish a theoretical foundation for further mechanism study on CGA biosynthesis and provide guidance for nutrient improvement in apple breeding programs.

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Section: Discussionsupporting

confidence: 92%

“…Relative studies have shown that HCT or C3H genes are key genes in CGA biosynthesis in fruits. For instance, two PpCYP45098A genes and four PpHCT genes showed the same expression patterns as the CGA accumulation in peach fruit [25]. In strawberries, the blue light was able to co-upregulate CGA biosynthesis and FvHCT gene expression [4].…”

Section: Discussionmentioning

confidence: 93%

Transcriptome Analysis Identifies Genes Associated with Chlorogenic Acid Biosynthesis during Apple Fruit Development

et al. 2023

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“…Transcription factors (TFs) are essential in regulating gene expression in plants. TFs can bind to various specific DNA elements upstream of the target gene and activate or inhibit the transcriptional activity of the target gene from regulating its spatial-specific expression [ 1 ]. In addition to hormone-related transcription factors (Figs.…”

Section: Resultsmentioning

confidence: 99%

“…Peach [ Prunus persica (L.) Batsch] is generally classified as a climacteric fruit, popular among consumers because of its beautiful appearance, delicate flesh aromatic flavor, and high nutritional value [ 1 ]. Fruit expansion stage of peach is the key stage of fruit yield and quality formation, which is a complex and genetically programmed process that results in a whole set of physiological, biochemical, and structural changes in the color, flavor, aroma, texture, and nutritional value [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Exogenous auxin regulates the growth and development of peach fruit at the expansion stage by mediating multiple-hormone signaling

Zhang,

Su,

Luo

et al. 2023

BMC Plant Biol

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Background Fruit expansion stage is crucial to fruit yield and quality formation, and auxin plays a significant role by mediating multi-hormone signals during fruit expansion. However, till now, it is still unclear of the molecular regulatory network during auxin-mediated peach fruit expansion. Results Here, exogenous NAA application markedly increased IAA content and drastically decreased ABA content at the fruit expansion stage. Correspondingly, NAA mainly induced the auxin biosynthesis gene (1 PpYUCCA) and early auxin-responsive genes (7PpIAA, 3 PpGH3, and 14 PpSAUR); while NAA down-regulated ABA biosynthesis genes (2 PpNCED, 1 PpABA3, and 1 PpAAO3). In addition, many DEGs involved in other plant hormone biosynthesis and signal transduction were significantly enriched after NAA treatment, including 7 JA, 7 CTK, 6 ETH, and 3 GA. Furthermore, we also found that NAA treatment down-regulated most of genes involved in the growth and development of peach fruit, including the cell wall metabolism-related genes (PpEG), sucrose metabolism-related genes (PpSPS), phenylalanine metabolism-related genes (PpPAL, Pp4CL, and PpHCT), and transcription factors (PpNAC, PpMADS-box, PpDof, PpSBP, and PpHB). Conclusion Overall, NAA treatment at the fruit expansion stage could inhibit some metabolism processes involved in the related genes in the growth and development of peach fruit by regulating multiple-hormone signaling networks. These results help reveal the short-term regulatory mechanism of auxin at the fruit expansion stage and provide new insights into the multi-hormone cascade regulatory network of fruit growth and development.

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“…Grapes and peaches are two economically important and widely cultivated fruit crops in the world [30,31]. Callhana [32] found that the expression levels of ACO genes in peaches varied among different tissue organs, mature fruits, and injured tissues.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Potential Action Mode Divergences of Homologous ACO1 Genes during the Organ Development and Ripening Process between Non-Climacteric Grape and Climacteric Peach

Luo,

Zhao,

et al. 2024

IJMS

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Ethylene is one crucial phytohormone modulating plants’ organ development and ripening process, especially in fruits, but its action modes and discrepancies in non-climacteric grape and climacteric peach in these processes remain elusive. This work is focused on the action mode divergences of ethylene during the modulation of the organ development and ripening process in climacteric/non-climacteric plants. We characterized the key enzyme genes in the ethylene synthesis pathway, VvACO1 and PpACO1, and uncovered that their sequence structures are highly conserved, although their promoters exhibit important divergences in the numbers and types of the cis-elements responsive to hormones, implying various responses to hormone signals. Subsequently, we found the two have similar expression modes in vegetative organ development but inverse patterns in reproductive ones, especially in fruits. Then, VvACO1 and PpACO1 were further validated in promoting fruit ripening functions through their transient over-expression/RNAi-expression in tomatoes, of which the former possesses a weaker role than the latter in the fruit ripening process. Our findings illuminated the divergence in the action patterns and function traits of the key VvACO1/PpACO1 genes in the tissue development of climacteric/non-climacteric plants, and they have implications for further gaining insight into the interaction mechanism of ethylene signaling during the modulation of the organ development and ripening process in climacteric/non-climacteric plants.

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Integrative analysis of the metabolome and transcriptome reveals the molecular mechanism of chlorogenic acid synthesis in peach fruit

Cited by 11 publications

References 45 publications

Transcriptome Analysis Identifies Genes Associated with Chlorogenic Acid Biosynthesis during Apple Fruit Development

Transcriptome Analysis Identifies Genes Associated with Chlorogenic Acid Biosynthesis during Apple Fruit Development

Exogenous auxin regulates the growth and development of peach fruit at the expansion stage by mediating multiple-hormone signaling

Characterization and Potential Action Mode Divergences of Homologous ACO1 Genes during the Organ Development and Ripening Process between Non-Climacteric Grape and Climacteric Peach

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