Transcriptome Analysis Identifies Genes Associated with Chlorogenic Acid Biosynthesis during Apple Fruit Development

Wang, Hao; Zheng, Xianbo; Zhan, Wenduo; Guo, Yingqing; Chen, Ming; Bai, Tuanhui; Jiao, Jian; Song, Chunhui; Song, Shangwei; Wang, Miaomiao

doi:10.3390/horticulturae9020217

Cited by 4 publications

(3 citation statements)

References 34 publications

(46 reference statements)

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“…(Cui et al 2022). As one of the major phenolic compounds, chlorogenic acid is abundant in various fruits and has a variety of antioxidant properties (Wang et al 2023).In this paper, most phenolic acids, including chlorogenic acid methyl ester, methyl 4hydroxybenzoate, coniferaldehyde, diethyl coniferin and moracin Y, were expressed at high levels in MG and at low levels in MR and MP (Fig. S3), and 68 phenolic acids were assigned to Cluster 6 and Cluster 7, representing metabolites with reduced levels (Fig.…”

Section: Flavonoids Of Mulberry Fruit At Different Developmental Stagesmentioning

confidence: 83%

“…S3), and 68 phenolic acids were assigned to Cluster 6 and Cluster 7, representing metabolites with reduced levels (Fig. 5), The reduction in these phenolic acid levels during fruit development and ripening is probably caused by enlarged fruit volume, hydrolysis of polyphenolic compounds, or reduced synthesis capacity (Wang et al 2023), which may help improve the taste avor of mulberry fruit.…”

Section: Flavonoids Of Mulberry Fruit At Different Developmental Stagesmentioning

confidence: 99%

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Analysis of Quality Changes in Mulberry Fruit at Three Developmental Stages Using Targeted Metabolomics Based on LC‒MS

Jiang

Cheng

et al. 2023

Tropical Plant Biol.

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Mulberry fruits are popular in many countries because of their good taste, high nutritional value and medicinal properties. Studying the metabolic pro le during fruit development can provide a better understanding of the changes in fruit quality. In this study, a widely targeted metabolomic approach using liquid chromatography-mass spectrometry (LC-MS) was used to investigate the widespread metabolic changes during fruit development and ripening in mulberry fruits and combined with multivariate statistical methods to screen for signi cant changed metabolites (SCMs) at different developmental stages. A total of 981 metabolites in 12 categories were detected in mulberry fruit at three developmental stages (i.e., the fruit expansion stage in which mulberry fruits are green (MG), the fruit color change stage in which they are red (MR) and the fruit ripening stage in which they are purple (MP)). There were signi cant changes in the metabolic pro le at the color change and ripening stages compared to the fruit expansion stage. Flavonoids increased mainly during fruit ripening and accumulated signi cantly during the mature stage. Large amounts of phenolic acids, lipids, and organic acids accumulated signi cantly during fruit expansion stage and decreased signi cantly during ripening. However, sugars accumulate signi cantly during ripening and are associated with the sweetness of mulberry fruit. Assessment of the levels of different sugar and acid components during fruit development revealed that fructose and glucose were the main sugars, and citric and malic acids were the main organic acids. Meanwhile, the sugar-acid ratio increased signi cantly, which in turn improved the avor quality of mulberry fruit. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the SCMs were mainly enriched in galactose metabolism, biosynthesis of secondary metabolites and avonoid biosynthesis metabolic pathways. The reliability of the metabolomics data was demonstrated by quantitative real-time PCR(qRT-PCR) validation of six genes encoding key enzymes. The results of this study provide insights into the rational utilization of mulberry fruit resources and the e cient development of functional fruit products.

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Section: Flavonoids Of Mulberry Fruit At Different Developmental Stagesmentioning

confidence: 83%

Section: Flavonoids Of Mulberry Fruit At Different Developmental Stagesmentioning

confidence: 99%

Analysis of Quality Changes in Mulberry Fruit at Three Developmental Stages Using Targeted Metabolomics Based on LC‒MS

Jiang

Cheng

et al. 2023

Tropical Plant Biol.

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“…Initially, phenylalanine ammonia-lyase (PAL) deaminates phenylalanine to form cinnamic acid. Subsequently, five known pathways exist for CGA biosynthesis ( Figure S1 ) [ 1 , 2 , 3 , 19 , 20 ]. The first pathway involves the formation of p -coumaroyl CoA from cinnamic acid by cinnamic acid 4-hydroxylase (C4H) and 4-coumarate-CoA ligase (4CL), followed by the consecutive catalysis of CYP98A (C3’H) and hydroxycinnamoyl transferase/hydroxycinnamoyl-CoA, quinate transferase (HCT/HQT), to produce caffeoyl quinic acid (CQA).…”

Section: Introductionmentioning

confidence: 99%

Uncovering the Role of Hydroxycinnamoyl Transferase in Boosting Chlorogenic Acid Accumulation in Carthamus tinctorius Cells under Methyl Jasmonate Elicitation

Liu,

Zhu,

Mohsin

et al. 2024

IJMS

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Chlorogenic acids (CGAs) are bioactive compounds widely used in the food, pharmaceutical, and cosmetic industries. Carthamus tinctorius is an important economic crop, and its suspension cells are rich in CGAs. However, little is known about the biosynthesis and regulation of CGAs in Carthamus tinctorius cells. This study first elucidated the regulatory mechanism of CGA biosynthesis in methyl jasmonate (MeJA)-treated Carthamus tinctorius cells and the role of the MeJA-responsive hydroxycinnamoyl transferase (HCT) gene in enhancing their CGA accumulation. Firstly, temporal changes in intracellular metabolites showed that MeJA increased the intracellular CGA content up to 1.61-fold to 100.23 mg·g−1. Meanwhile, 31 primary metabolites showed significant differences, with 6 precursors related to increasing CGA biosynthesis. Secondly, the transcriptome data revealed 3637 new genes previously unannotated in the Carthamus tinctorius genome and 3653 differentially expressed genes. The genes involved in the plant signaling pathway and the biosynthesis of CGAs and their precursors showed a general up-regulation, especially the HCT gene family, which ultimately promoted CGA biosynthesis. Thirdly, the expression of a newly annotated and MeJA-responsive HCT gene (CtHCT, CtNewGene_3476) was demonstrated to be positively correlated with CGA accumulation in the cells, and transient overexpression of CtHCT enhanced CGA accumulation in tobacco. Finally, in vitro catalysis kinetics and molecular docking simulations revealed the ability and mechanism of the CtHCT protein to bind to various substrates and catalyze the formation of four hydroxycinnamic esters, including CGAs. These findings strengthened our understanding of the regulatory mechanism of CGA biosynthesis, thereby providing theoretical support for the efficient production of CGAs.

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Integrated metabolomic and transcriptomic analysis reveals biosynthesis mechanism of flavone and caffeoylquinic acid in chrysanthemum

Lu,

Yan,

Zhang

et al. 2024

BMC Genomics

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Transcriptome Analysis Identifies Genes Associated with Chlorogenic Acid Biosynthesis during Apple Fruit Development

Cited by 4 publications

References 34 publications

Analysis of Quality Changes in Mulberry Fruit at Three Developmental Stages Using Targeted Metabolomics Based on LC‒MS

Analysis of Quality Changes in Mulberry Fruit at Three Developmental Stages Using Targeted Metabolomics Based on LC‒MS

Uncovering the Role of Hydroxycinnamoyl Transferase in Boosting Chlorogenic Acid Accumulation in Carthamus tinctorius Cells under Methyl Jasmonate Elicitation

Integrated metabolomic and transcriptomic analysis reveals biosynthesis mechanism of flavone and caffeoylquinic acid in chrysanthemum

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